Name: Amplite® Fluorimetric Alanine Aminotransferase Assay Kit
Brand: AAT Bioquest
SKU: 13802
Price: 355 USD
Availability: InStock

Amplite® Fluorimetric Alanine Aminotransferase Assay Kit

Amplite® Fluorimetric Alanine Aminotransferase Assay Kit is a fluorimetric assay kit for quantification of alanine aminotransferase activity in biological samples.

Detection Principle: Measures glutamate generated by the ALT-catalyzed reaction.
Sensitivity: Detects ALT activity down to 4 mU/mL.
Reaction Chemistry: Uses alanine and α-ketoglutarate as ALT substrates.
Instrument Compatibility: Fluorescence detection at Ex 540/Em 590 nm.
Applications: Suitable for ALT measurement across a variety of biological samples.

ALT dose response was measured with Amplite® Fluorimetric Alanine Aminotransferase Assay Kit in a 96-well black plate using a Gemini fluorescence microplate reader (Molecular Devices).

Protocol

SDS

Catalog	Size	Price	Quantity
13802	200 Tests	Price

Overview

Alanine aminotransferase (ALT), also called serum glutamate pyruvic transaminase (GPT), is a member of transferase family. It catalyzes the reversible transfer of an alpha-amino group between alanine and glutamate, and is an important enzyme in amino acid metabolism. ALT is found mainly in liver and small amount in heart, muscle, and kidneys. In healthy subjects, serum ALT levels are low. However, when cells are damaged, such as acute and chronic hepatitis, obstructive jaundice, carcinoma of liver, myocardial infarction, ALT may leak into the blood stream and the ALT levels are significantly elevated. Therefore, determination of serum ALT level has great clinical and diagnostic significance. Amplite® Fluorimetric Alanine Aminotransferase Assay Kit provides a quick and sensitive method for the measurement of ALT in various biological samples. ALT catalyzes the reaction of alanine and α-ketoglutarate to pyruvate and glutamate. The product glutamate is measured by the generation of a red fluorescent product through an enzyme coupled reaction cycle. The signal can be read by a fluorescence microplate reader. With the Amplite® Fluorimetric Alanine Aminotransferase Assay Kit as little as 4 mU/mL ALT was detected in a 100 µL reaction volume. The assay is robust, and can be readily adapted for a wide variety of applications.

Citations

View all 2 citations: Citation Explorer

Reparative Efficacy of Liposome-Encapsulated Oleanolic Acid against Liver Inflammation Induced by Fine Ambient Particulate Matter and Alcohol in Mice

Authors:

Wei, Ching-Ting and Wang, Yu-Wen and Wu, Yu-Chiuan and Lin, Li-Wei and Chen, Chia-Chi and Chen, Chun-Yin and Kuo, Shyh-Ming

Journal:

Pharmaceutics (2022): 1108

The receptor for advanced glycation endproducts (RAGE) contributes to severe inflammatory liver injury in mice

Authors:

Weinhage, Toni and Wirth, Timo and Sch{\"u}tz, Paula and Becker, Philipp and Lueken, Aloys and Skryabin, Boris V and Wittkowski, Helmut and Foell, Dirk

Journal:

Frontiers in immunology (2020): 1157

References

View all 226 references: Citation Explorer

Pegylated interferon alpha-2b plus ribavirin for Japanese chronic hepatitis C patients with normal alanine aminotransferase

Authors:

Kainuma M, Furusyo N, Azuma K, Kajiwara E, Takahashi K, Nomura H, Tanabe Y, Satoh T, Maruyama T, Nakamuta M, Kotoh K, Shimoda S, Hayashi J.

Journal:

Hepatol Res (2012): 33

Coexistence of non-alcoholic fatty liver disease with elevated alanine aminotransferase is associated with insulin resistance in young Han males

Authors:

Wang R, Lu Q, Feng J, Yin F, Qin C, Liu B, Liu Y, Liu X.

Journal:

Endocrine (2012): 70

Levels of Alanine Aminotransferase Confound Use of Transient Elastography to Diagnose Fibrosis in Patients with Chronic HCV Infection

Authors:

Tapper EB, Cohen EB, Patel K, Bacon B, Gordon S, Lawitz E, Nelson D, Nasser IA, Challies T, Afdhal N.

Journal:

Clin Gastroenterol Hepatol. (2012)

Gender differences in healthy ranges for serum alanine aminotransferase levels in adolescence

Authors:

Poustchi H, George J, Esmaili S, Esna-Ashari F, Ardalan G, Sepanlou SG, Alavian SM.

Journal:

PLoS One (2011): e21178

Updated thresholds for alanine aminotransferase do not exclude significant histological disease in chronic hepatitis C

Authors:

Sanai FM, Helmy A, Dale C, Al-Ashgar H, Abdo AA, Katada K, AlMana H, Saadeh M, Al-Hussaini H, AlQuaiz M, Hashem A, AlSwat K, Bzeizi KI, Marotta PJ.

Journal:

Liver Int (2011): 1039

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Fluorescence microplate reader
Excitation	540 nm
Emission	590 nm
Cutoff	570 nm
Recommended plate	Solid black

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Page updated on October 6, 2026

Component A: ALT Enzyme Mix	1 bottle (lyophilized powder)
Component B: ALT Assay Buffer	bottle (10 mL)
Component C: NAD	1 vial
Component D: ALT Positive Control	1 vial (10 U)