Transferases
Transferases are a class of enzymes that catalyze the transfer of specific functional groups (e.g. a methyl or glycosyl group) from a donor molecule to an acceptor molecule. They are involved in a myriad of vital cellular processes including the transfer of thiol esters, metabolism of tryptophan and the regulation of pyruvate dehydrogenase (PDH). Transferases are classified primarily based on the type of biochemical group transferred, there are 10 categories in all comprising over 400 unique enzymes.
Common classifications include:
- Methyltransferases and formyltransferase - enzymes that transfer single-carbon groups methyl (-CH3) and formyl (-CHO) groups
- Transketolase and transaldolase - enzymes that transfer ketone or aldehyde group
- Oxi aminotransferase or transaminase - enzymes that transfer nitrogenous groups
- Sulfotransferase - enzymes that transfer sulfur-containing groups
Table of Contents
- Alanine Aminotransferase
- 1.1 Amplite® Fluorimetric Alanine Aminotransferase Assay
- 1.2 Amplite® Colorimetric Alanine Aminotransferase Assay
- Aspartate Aminotransferase
- 2.1 Amplite® Fluorimetric Aspartate Aminotransferase Assay
- 2.2 Amplite® Colorimetric Aspartate Aminotransferase Assay
- HDAC Activity Assay
- Product Ordering Information
Alanine Aminotransferase
ALT dose response was measured with Amplite® Fluorimetric Alanine Aminotransferase Assay Kit (Cat No. 13802) in a 96-well black plate using a Gemini fluorescence microplate reader.
Amplite® Fluorimetric Alanine Aminotransferase Assay
ALT dose response was measured with Amplite® Colorimetric Alanine Aminotransferase Assay Kit (Cat No. 13803) in a 96-well white, clear-bottom plate using a SpectraMax microplate reader (Molecular Devices).
Amplite® Colorimetric Alanine Aminotransferase Assay
The product glutamate can also be measured using Amplite® Colorimetric Alanine Aminotransferase Assay Kit (Cat No. 13803) by the generation of a blue color product through an enzyme coupled reaction cycle. The signal can be read using an absorbance microplate reader at ~575 nm or by the absorbance ratio of A570 nm to A610 nm to increase assay sensitivity. With Amplite® Colorimetric Alanine Aminotransferase Assay Kit, as little as 10 mU/mL ALT was detected in a 100 µL reaction volume.
Aspartate Aminotransferase
AST dose response was measured with Amplite® Fluorimetric Aspartate Aminotransferase Assay Kit (Cat No. 13800) in a 96-well black plate using a Gemini fluorescence microplate reader.
Amplite® Fluorimetric Aspartate Aminotransferase Assay
AST dose response was measured with Amplite® Colorimetric Aspartate Aminotransferase Assay Kit (Cat No. 13801) in a 96-well white, clear-bottom plate using a SpectraMax microplate reader (Molecular Devices).
Amplite® Colorimetric Aspartate Aminotransferase Assay
The product glutamate can also be measured using Amplite® Colorimetric Aspartate Aminotransferase Assay Kit (Cat No. 13801) by the generation of a blue color product through an enzyme coupled reaction cycle. The signal can be read using an absorbance microplate reader at ~575 nm or by the absorbance ratio of A570 nm to A610 nm to increase assay sensitivity. With Amplite® Colorimetric Aspartate Aminotransferase Assay Kit, as little as 3 mU/mL AST was detected in a 100 µL reaction volume.
HDAC Activity Assay
HDAC activity in HeLa nuclear extract was measured with Amplite® Fluorimetric HDAC Activity Assay Kit (blue) was compared with Vendor X (red) and Vendor Y (green), both of which use Ac-RGK(Ac)-R110 peptide substrate. The signal/background ratio of the HDAC activity measured with Amplite® Fluorimetric HDAC Activity Assay Kit is more than 10 times higher than those of Vendors X and Y.
Amplite® Fluorimetric HDAC Activity Assay
Amplite® Fluorimetric HDAC Activity Assay Kit (Cat No. 13601) provides a quick, convenient, and sensitive method for the detection of HDAC activity. This kit uses our non-peptide HDAC Green™ substrate that is much more sensitive than conventional peptide based HDAC substrates such as Ac-RGK(Ac)-R110, Ac-RGK(Ac)-AMC and Ac-RGK(Ac)-AFC. In addition, HDAC Green™ substrate is also much more resistant to protease hydrolysis than other commercial peptide-based HDAC substrates. Our kit can be used for measuring HDAC activity in cell lysates or HDAC inhibitor screening with cell extracts or purified enzymes. The long wavelength emission of the HDAC Green™ substrate makes the assay less interfered from compounds and cell components. HDAC activity is monitored with excitation at 490 nm and emission at 525 nm.
Product Ordering Information
Table 1. Ordering Info for Transferase Assays Products
Cat# ▲ ▼ | Product Name ▲ ▼ | Unit Size ▲ ▼ | Price ▲ ▼ |
13601 | Amplite® Fluorimetric HDAC Activity Assay Kit *Green Fluorescence* | 200 Tests | 205 |
13800 | Amplite® Fluorimetric Aspartate Aminotransferase (AST) Assay Kit | 200 Tests | 310 |
13801 | Amplite® Colorimetric Aspartate Aminotransferase (AST) Assay Kit | 200 Tests | 310 |
13802 | Amplite® Fluorimetric Alanine Aminotransferase Assay Kit | 200 Tests | 310 |
13803 | Amplite® Colorimetric Alanine Aminotransferase Assay Kit *Blue Color* | 200 Tests | 310 |