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Amplite® Fluorimetric Beta-Galactosidase Assay Kit
Red Fluorescence
E. coli beta-galactosidase is a 464 kD tetramer. Each unit of beta-galactosidase consists of five domains, the third of which is the active site. It is an essential enzyme in cells. Deficiencies of this enzyme can result in galactosialidosis or Morquio B syndrome. In E. coli, beta-galactosidase is produced by the activation of LacZ operon. Detection of LacZ expression has become routine to the point of detection of as few as 5 copies of beta-galactosidase per cell. This kit uses a red fluorogenic galactosidase substrate that can sensitively distinguish LacZ+ from LacZ- cells. The non-fluorescent substate generates a strongly fluorescent product upon reaction with galactosidase. It can be used either for detecting galactosidase conjugates in ELISA type assay systems or for monitoring LacZ gene expression in cells. Amplite® Fluorimetric Beta-Galactosidase Assay Kit comes with all the essential components with an optimized assay protocol. It can be used with a fluorescence microplate reader, a fluorescence microscope, or a flow cytometer. It might also be used for screening galactosidase inhibitors or inducers.
β-galactosidase dose response was measured with Amplite™ Fluorimetric beta-Galactosidase Assay Kit in a Costar solid black 96-well plate using Gemini fluorescence microplate reader (Molecular Devices). 
β-galactosidase dose response was measured with Amplite™ Fluorimetric beta-Galactosidase Assay Kit in a Costar solid black 96-well plate using Gemini fluorescence microplate reader (Molecular Devices). 
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
12603200 Tests
Price
 
Spectral properties
Absorbance (nm)570
Extinction coefficient (cm -1 M -1)
65000
1
Excitation (nm)571
Emission (nm)584
Quantum yield
0.75
1
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12352200
Instrument settings
Fluorescence microplate reader
Excitation540 nm
Emission590 nm
Cutoff570 nm
Recommended plateSolid black
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Page updated on September 25, 2025