Name: Amplite® Fluorimetric Beta-Galactosidase Assay Kit *Red Fluorescence*
Brand: AAT Bioquest
SKU: 12603
Price: 355 USD
Availability: InStock

Amplite® Fluorimetric Beta-Galactosidase Assay Kit

Red Fluorescence

Amplite® Fluorimetric Beta-Galactosidase Assay Kit *Red Fluorescence* is a fluorimetric assay kit for detection of beta-galactosidase activity in cells and ELISA-type assay systems.

Detection Principle: Uses a red fluorogenic galactosidase substrate.
Signal Advantage: Distinguishes LacZ+ from LacZ− cells.
Detection Chemistry: Uses resorufin β-galactoside as the substrate.
Instrument Compatibility: Compatible with microplate readers, fluorescence microscopes, and flow cytometers.
FeatureApplications: Supports LacZ expression monitoring and galactosidase-conjugate detection.

&beta;-galactosidase dose response was measured with Amplite&trade; Fluorimetric beta-Galactosidase Assay Kit in a Costar solid black 96-well plate using Gemini fluorescence microplate reader (Molecular Devices).

Protocol

SDS

Catalog	Size	Price	Quantity
12603	200 Tests	Price

Overview

E. coli beta-galactosidase is a 464 kD tetramer. Each unit of beta-galactosidase consists of five domains, the third of which is the active site. It is an essential enzyme in cells. Deficiencies of this enzyme can result in galactosialidosis or Morquio B syndrome. In E. coli, beta-galactosidase is produced by the activation of LacZ operon. Detection of LacZ expression has become routine to the point of detection of as few as 5 copies of beta-galactosidase per cell. This kit uses a red fluorogenic galactosidase substrate that can sensitively distinguish LacZ+ from LacZ- cells. The non-fluorescent substate generates a strongly fluorescent product upon reaction with galactosidase. It can be used either for detecting galactosidase conjugates in ELISA type assay systems or for monitoring LacZ gene expression in cells. Amplite® Fluorimetric Beta-Galactosidase Assay Kit comes with all the essential components with an optimized assay protocol. It can be used with a fluorescence microplate reader, a fluorescence microscope, or a flow cytometer. It might also be used for screening galactosidase inhibitors or inducers.

Citations

View all 2 citations: Citation Explorer

IL-10-induced senescence of HSCs enhances the activation and cytotoxicity of NK cells via NKG2D pathway to attenuate liver fibrosis in vivo and vitro

Authors:

Chen, Yizhen and Chen, Yunxin and Guo, Qilan and Huang, Yixuan and Chen, Fenglin and Huang, Yuehong

BZLF1 Attenuates Transmission of Inflammatory Paracrine Senescence in Epstein-Barr Virus-Infected Cells by Downregulating Tumor Necrosis Factor Alpha

Authors:

Long, Xubing and Li, Yuqing and Yang, Mengtian and Huang, Lu and Gong, Weijie and Kuang, Ersheng

Journal:

Journal of Virology (2016): 7880--7893

References

View all 23 references: Citation Explorer

Microfluidic sequential injection analysis in a short capillary

Authors:

Du WB, Fang Q, Fang ZL.

Journal:

Anal Chem (2006): 6404

Cellular logic with orthogonal ribosomes

Authors:

Rackham O, Chin JW.

Journal:

J Am Chem Soc (2005): 17584

New transport peptides broaden the horizon of applications for peptidic pharmaceuticals

Authors:

Langedijk JP, Olijhoek T, Schut D, Autar R, Meloen RH.

Journal:

Mol Divers (2004): 101

Integration of on-column immobilized enzyme reactor in microchip electrophoresis

Authors:

Park SS, Cho SI, Kim MS, Kim YK, Kim BG.

Journal:

Electrophoresis (2003): 200

Model for focal demyelination of the spinal dorsal columns of transgenic MBP-LacZ mice by phototargeted ablation of oligodendrocytes

Authors:

V, undefined and erluit JL, Bourque JA, Peterson AC, Tetzlaff W.

Journal:

J Neurosci Res (2000): 28

Spectral properties

Absorbance (nm)	570
Extinction coefficient (cm ^-1 M ^-1)	65000 ¹
Excitation (nm)	571
Emission (nm)	584
Quantum yield	0.75 ¹

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Fluorescence microplate reader
Excitation	540 nm
Emission	590 nm
Cutoff	570 nm
Recommended plate	Solid black

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Page updated on October 8, 2026

Component A: Resorufin β-Galactoside	1 vial
Component B: Reaction Buffer	1 bottle (20 mL)
Component C: Stop Buffer	1 vial (10 mL)
Component D: Lysis Buffer	1 vial (10 mL)
Component E: DMSO	1 vial (100 µL)
Component F: β-Mercaptoethanol	1 vial (100 µL)