Amplite® Fluorimetric Hydrogen Peroxide Assay Kit *Near Infrared Fluorescence*

Amplite® Fluorimetric Hydrogen Peroxide Assay Kit

Near Infrared Fluorescence

Amplite® Fluorimetric Hydrogen Peroxide Assay Kit *Near Infrared Fluorescence* is a fluorimetric assay kit for quantification of hydrogen peroxide in solutions and cell extracts.

Detection Principle: Uses an HRP-coupled reaction to detect H₂O₂.
Signal Advantage: Near-infrared fluorescence minimizes biological sample autofluorescence.
Workflow: Mix-and-read format compatible with HTS liquid handling.
Instrument Compatibility: Fluorescence detection at Ex/Em 640/680 nm.

Hydrogen Peroxide dose response was measured in a solid black 96-well plate with Amplite® Fluorimetric Hydrogen Peroxide Assay Kit.

Protocol

SDS

Catalog	Size	Price	Quantity
11502	500 Tests	Price

Overview

Hydrogen peroxide (H2O2) is a reactive oxygen metabolic by-product that serves as a key regulator for a number of oxidative stress-related states. It is involved in a number of biological events that have been linked to asthma, atherosclerosis, diabetic vasculopathy, osteoporosis, a number of neurodegenerative diseases and Down's syndrome. Perhaps the most intriguing aspect of H2O2 biology is the recent report that antibodies have the capacity to convert molecular oxygen into hydrogen peroxide to contribute to the normal recognition and destruction processes of the immune system. Measurement of this reactive species will help to determine how oxidative stress modulates varied intracellular pathways. This Amplite® Hydrogen Peroxide Assay Kit uses our unique Amplite® IR peroxidase substrate to quantify hydrogen peroxide in solutions and cell extracts. Amplite® IR generates the fluorescence that is pH-independent from pH 4 to 10. Thus it is superior alternative to ADHP (Amplex Red™) for the detections that require low pH where ADHP (Amplex Red™) has reduced fluorescence. In addition, Amplite® IR generates a product that has maximum absorption of 647 nm with maximum emission at 670 nm. This near infrared absorption and fluorescence minimize the assay background that is often caused by the autofluorescence of biological samples that rarely absorb light beyond 600 nm. It can also be used to detect a variety of oxidase activities through enzyme-coupled reactions. The kit is an optimized 'mix and read' assay that is compatible with HTS liquid handling instruments.

Citations

View all 12 citations: Citation Explorer

A Dual-Functional PEG-Tyrosine Hydrogel with Photothermal Effect and Antioxidant Capacity for Cancer Therapy and Tissue Regeneration

Authors:

Zhu, Haitao and Shen, Yunfan and Wu, Zhebin and Yi, Yide and Chen, Yuanfei and Xu, Wenjin and Wang, Xin and Tsai, Hsiang-I and Wang, Dongqing and Liao, Xiang and others,

Journal:

Regenerative Biomaterials (2026): rbag034

An Oxygen Supply Strategy for Sonodynamic Therapy in Tuberculous Granuloma Lesions Using a Catalase-Loaded Nanoplatform

Authors:

Hu, Can and Qiu, Yan and Guo, Jiajun and Cao, Yuchao and Li, Dairong and Du, Yonghong

Journal:

International Journal of Nanomedicine (2023): 6257--6274

Redox-Active Polyphenol Nanoparticles Deprive Endogenous Glutathione of Electrons for ROS Generation and Tumor Chemodynamic Therapy

Authors:

Wang, Yifei and Wang, Jia and Jiao, Yunke and Chen, Kangli and Chen, Tianhao and Wu, Xin-Ping and Jiang, Xingwu and Bu, Wenbo and Liu, Changsheng and Qu, Xue

Journal:

Acta Biomaterialia (2023)

Pleiotropic Microenvironment Remodeling Micelles for Cerebral Ischemia-Reperfusion Injury Therapy by Inhibiting Neuronal Ferroptosis and Glial Overactivation

Authors:

Li, Chao and Wu, Yuxing and Chen, Qinjun and Luo, Yifan and Liu, Peixin and Zhou, Zheng and Zhao, Zhenhao and Zhang, Tongyu and Su, Boyu and Sun, Tao and others,

Journal:

ACS nano (2023)

Macrophage metabolism reprogramming EGCG-Cu coordination capsules delivered in polyzwitterionic hydrogel for burn wound healing and regeneration

Authors:

Li, Qinghua and Song, Huijuan and Li, Shuangyang and Hu, Pengbo and Zhang, Chuangnian and Zhang, Ju and Feng, Zujian and Kong, Deling and Wang, Weiwei and Huang, Pingsheng

Journal:

Bioactive Materials (2023): 251--264

References

View all 152 references: Citation Explorer

Simple and rapid determination of hydrogen peroxide using phosphine-based fluorescent reagents with sodium tungstate dihydrate

Authors:

Onoda M, Uchiyama T, Mawatari K, Kaneko K, Nakagomi K.

Journal:

Anal Sci (2006): 815

Effect of antisense oligonucleotide against Smac/DIABLO on inhibition of hydrogen peroxide induced myocardial apoptosis of neonatal rats

Authors:

Liang PF, Huang XY, Long JH, Xiao MZ, Yang XH, Zhang PH.

Journal:

Zhonghua Shao Shang Za Zhi (2006): 175

Cardioprotective role of endogenous hydrogen peroxide during ischemia-reperfusion injury in canine coronary microcirculation in vivo

Authors:

Yada T, Shimokawa H, Hiramatsu O, Haruna Y, Morita Y, Kashihara N, Shinozaki Y, Mori H, Goto M, Ogasawara Y, Kajiya F.

Journal:

Am J Physiol Heart Circ Physiol (2006): H1138

Specific aquaporins facilitate the diffusion of hydrogen peroxide across membranes

Authors:

Bienert GP, Moller AL, Kristiansen KA, Schulz A, Moller IM, Schjoerring JK, Jahn TP.

Journal:

J Biol Chem. (2006)

Enzymatic oxidation of dipyridamole in homogeneous and micellar solutions in the horseradish peroxidase-hydrogen peroxide system

Authors:

Almeida LE, Imasato H, Tabak M.

Journal:

Biochim Biophys Acta (2006): 216

Spectral properties

Excitation (nm)	648
Emission (nm)	668

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Fluorescence microplate reader
Excitation	640 nm
Emission	680 nm
Cutoff	665 nm
Recommended plate	Solid black

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Fax
Email	sales@aatbio.com
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Custom size	Inquire
Technical support	Contact us
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Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on October 7, 2026

Component A: Amplite® IR Peroxidase Substrate	1 vial
Component B: H2O2	1 vial (3% stabilized solution, 200 µL)
Component C: Assay Buffer	1 bottle (100 mL)
Component D: Horseradish Peroxidase	1 vial (20 units)
Component E: DMSO	1 vial (0.5 mL)