Amplite® Fluorimetric Melanin Assay Kit

Amplite® Fluorimetric Melanin Assay Kit is a fluorimetric assay kit for quantification of melanin content in cells and other samples.

Detection Principle: Uses a substrate that generates a fluorescent product upon reaction with melanin.
Signal Response: Fluorescence intensity is proportional to the amount of melanin in the sample.
Workflow: Incubates at room temperature for 30–60 minutes.
Instrument Compatibility: Fluorescence detection at Ex/Em 470/550 nm with a microplate.

Melanin dose response was measured with Amplite® Fluorimetric Melanin Assay Kit in a 96-well black plate using a Gemini microplate reader (Molecular Devices). Equal volume of melanin standards and Signal Enhancer were added and incubated for 45 minutes at room temperature. The signal was acquired at Ex/Em = 470/550 nm (cut off at 515 nm).

Protocol

SDS

COA

Catalog	Size	Price	Quantity
11310	100 Tests	Price

Overview

Melanins have very diverse roles and functions in various organisms. Since melanins are an important biomarker, the accurate and sensitive determination of melanins has become a critical task for biomedical research and diagnostic applications. To address this unmet need, we have developed a robust fluorescence-based melanin assay. Amplite® Fluorimetric Melanin Assay Kit uses a substrate that generates a fluorescent product upon reaction with melanins. Its fluorescence intensity is proportional to the amount of melanins in a sample. Amplite® Fluorimetric Melanin Assay Kit provides a simple and effective method to measure melanin content in cells and other samples. The plate-based assay format is designed to use with a fluorescent microplate reader.

Citations

View all 11 citations: Citation Explorer

The anaerobic fungus Neocallimastix californiae shifts metabolism and produces melanin in response to lignin-derived aromatic compounds

Authors:

Lankiewicz, Thomas S and Amer, Bashar and Baidoo, Edward EK and Leggieri, Patrick A and O’Malley, Michelle A

Journal:

Biotechnology for Biofuels and Bioproducts (2025): 96

Nasal Cavity Administration of Melanin-Concentrating Hormone Improves Memory Impairment in Memory-Impaired and Alzheimer's Disease Mouse Models

Authors:

Oh, S. T., Liu, Q. F., Jeong, H. J., Lee, S., Samidurai, M., Jo, J., Pak, S. C., Park, H. J., Kim, J., Jeon, S.

Journal:

Mol Neurobiol (2019): ersion="1.0" encoding="UTF-8" ?>11310.enlEndN

The Melanin-concentrating Hormone System in Human, Rodent and Avian Brain

Authors:

Bencze, J., Pocsai, K., Murnyak, B., Gergely, P. A., Juhasz, B., Szilvassy, Z., Hortobagyi, T.

Journal:

Open Med (Wars) (2018): 264-269

Novel Neuroprotective Effects of Melanin-Concentrating Hormone in Parkinson's Disease

Authors:

Park, J. Y., Kim, S. N., Yoo, J., Jang, J., Lee, A., Oh, J. Y., Kim, H., Oh, S. T., Park, S. U., Kim, J., Park, H. J., Jeon, S.

Journal:

Mol Neurobiol (2017): 7706-7721

Melanin or a Melanin-Like Substance Interacts with the N-Terminal Portion of Prion Protein and Inhibits Abnormal Prion Protein Formation in Prion-Infected Cells

Authors:

Hamanaka, T., Nishizawa, K., Sakasegawa, Y., Oguma, A., Teruya, K., Kurahashi, H., Hara, H., Sakaguchi, S., Doh-Ura, K.

Journal:

J Virol (2017): se name="11310.enl" path="C:\Users\aatbi\Drop

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Fluorescence microplate reader
Excitation	470 nm
Emission	550 nm
Cutoff	515 nm
Recommended plate	Solid black

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Page updated on October 8, 2026

Component A: Melanin Standard	1 vial
Component B: Assay Buffer	1 bottle (20 mL)
Component C: Signal Enhancer	1 bottle (5 mL)
Component D: DMSO	1 vial (200 µL)