Amplite® Fluorimetric Melanin Assay Kit

Melanins have very diverse roles and functions in various organisms. Since melanins are an important biomarker, the accurate and sensitive determination of melanins has become a critical task for biomedical research and diagnostic applications. To address this unmet need, we have developed a robust fluorescence-based melanin assay. Amplite® Fluorimetric Melanin Assay Kit uses a substrate that generates a fluorescent product upon reaction with melanins. Its fluorescence intensity is proportional to the amount of melanins in a sample. Amplite® Fluorimetric Melanin Assay Kit provides a simple and effective method to measure melanin content in cells and other samples. The plate-based assay format is designed to use with a fluorescent microplate reader.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
11310	100 Tests	Price

Citations

View all 11 citations: Citation Explorer

The anaerobic fungus Neocallimastix californiae shifts metabolism and produces melanin in response to lignin-derived aromatic compounds

Authors:

Lankiewicz, Thomas S and Amer, Bashar and Baidoo, Edward EK and Leggieri, Patrick A and O’Malley, Michelle A

Journal:

Biotechnology for Biofuels and Bioproducts (2025): 96

Nasal Cavity Administration of Melanin-Concentrating Hormone Improves Memory Impairment in Memory-Impaired and Alzheimer's Disease Mouse Models

Authors:

Oh, S. T., Liu, Q. F., Jeong, H. J., Lee, S., Samidurai, M., Jo, J., Pak, S. C., Park, H. J., Kim, J., Jeon, S.

Journal:

Mol Neurobiol (2019): ersion="1.0" encoding="UTF-8" ?>11310.enlEndN

The Melanin-concentrating Hormone System in Human, Rodent and Avian Brain

Authors:

Bencze, J., Pocsai, K., Murnyak, B., Gergely, P. A., Juhasz, B., Szilvassy, Z., Hortobagyi, T.

Journal:

Open Med (Wars) (2018): 264-269

Novel Neuroprotective Effects of Melanin-Concentrating Hormone in Parkinson's Disease

Authors:

Park, J. Y., Kim, S. N., Yoo, J., Jang, J., Lee, A., Oh, J. Y., Kim, H., Oh, S. T., Park, S. U., Kim, J., Park, H. J., Jeon, S.

Journal:

Mol Neurobiol (2017): 7706-7721

Melanin or a Melanin-Like Substance Interacts with the N-Terminal Portion of Prion Protein and Inhibits Abnormal Prion Protein Formation in Prion-Infected Cells

Authors:

Hamanaka, T., Nishizawa, K., Sakasegawa, Y., Oguma, A., Teruya, K., Kurahashi, H., Hara, H., Sakaguchi, S., Doh-Ura, K.

Journal:

J Virol (2017): se name="11310.enl" path="C:\Users\aatbi\Drop

Storage, safety and handling

Intended use

Research Use Only (RUO)

Instrument settings

Fluorescence microplate reader
Excitation	470 nm
Emission	550 nm
Cutoff	515 nm
Recommended plate	Solid black

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Page updated on October 13, 2025

Component A: Melanin Standard	1 vial
Component B: Assay Buffer	1 bottle (20 mL)
Component C: Signal Enhancer	1 bottle (5 mL)
Component D: DMSO	1 vial (200 µL)