Name: Amplite® Fluorimetric Peroxidase (HRP) Assay Kit *Red Fluorescence*
Brand: AAT Bioquest
SKU: 11552
Price: 237 USD
Availability: InStock

Amplite® Fluorimetric Peroxidase (HRP) Assay Kit

Red Fluorescence

Amplite® Fluorimetric Peroxidase (HRP) Assay Kit *Red Fluorescence* is a fluorimetric assay kit for detection of horseradish peroxidase activity in a microplate format.

Detection Principle: Measures HRP activity through H₂O₂-dependent oxidation of Amplite® Red.
Workflow: Quick 10-minute, one-step, homogeneous, no-wash assay.
Instrument Compatibility: Fluorescence detection at Ex/Em 540/590 nm; absorbance can also be read at 576 ± 5 nm.
Applications: Suitable for ELISAs, enzyme kinetics studies, and high-throughput screening of oxidase inhibitors.

HRP dose response was measured with Amplite Fluorimetric Peroxidase Assay Kit in a black plate using a Gemini fluorescence microplate reader (Molecular Devices).

Protocol

SDS

Catalog	Size	Price	Quantity
11552	500 Tests	Price

Overview

Peroxidase is a small molecule (MW ~40 KD) that can usually be conjugated to an antibody in a 4:1 ratio. Due to its small size, it rarely causes steric hindrance problem with antibody/antigen complex formation. Peroxidase is inexpensive compared to other labeling enzymes. The major disadvantage associated with peroxidase is their low tolerance to many preservatives such as sodium azide that inactivates peroxidase activity even at low concentration. HRP conjugates are extensively used as secondary detection reagents in ELISAs, immuno-histochemical techniques and Northern, Southern and Western blot analyses. We offer this quick (10 min) HRP assay in a one-step, homogeneous, no wash assay system. The kit can be used for ELISAs, characterizing kinetics of enzyme reaction and high throughput screening of oxidase inhibitors, etc. The kit provides an optimized 'mix and read' assay protocol that is compatible with HTS liquid handling instruments.

Citations

View all 2 citations: Citation Explorer

Identification of acetylcholinesterase inhibitors using homogenous cell-based assays in quantitative high-throughput screening platforms

Authors:

Li, Shuaizhang and Huang, Ruili and Solomon, Samuel and Liu, Yitong and Zhao, Bin and Santillo, Michael F and Xia, Menghang

Journal:

Biotechnology Journal (2017): 1600715

Identification of acetylcholinesterase inhibitors using homogenous cell-based assays in quantitative high-throughput screening platforms

Authors:

Li, Shuaizhang and Huang, Ruili and Solomon, Samuel and Liu, Yitong and Zhao, Bin and Santillo, Michael F and Xia, Menghang

Journal:

Biotechnology journal (2017): 1600715

References

View all 49 references: Citation Explorer

Horseradish peroxidase-driven fluorescent labeling of nanotubes with quantum dots

Authors:

Didenko VV, Baskin DS.

Journal:

Biotechniques (2006): 295

Enzymatic oxidation of dipyridamole in homogeneous and micellar solutions in the horseradish peroxidase-hydrogen peroxide system

Authors:

Almeida LE, Imasato H, Tabak M.

Journal:

Biochim Biophys Acta (2006): 216

Recent advances in catalytic peroxidase histochemistry

Authors:

Krieg R, Halbhuber KJ.

Journal:

Cell Mol Biol (Noisy-le-grand) (2003): 547

Vesicular transport route of horseradish C1a peroxidase is regulated by N- and C-terminal propeptides in tobacco cells

Authors:

Matsui T, Nakayama H, Yoshida K, Shinmyo A.

Journal:

Appl Microbiol Biotechnol (2003): 517

Synthesis and purification of horseradish peroxidase-labeled oligonucleotides for tyramide-based fluorescence in situ hybridization

Authors:

van Gijlswijk RP, van de Corput MP, Bezrookove V, Wiegant J, Tanke HJ, Raap AK.

Journal:

Histochem Cell Biol (2000): 175

Spectral properties

Excitation (nm)	571
Emission (nm)	584

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Absorbance microplate reader
Absorbance	576 ± 5 nm
Recommended plate	Clear bottom

Fluorescence microplate reader
Excitation	540 nm
Emission	590 nm
Cutoff	575 nm
Recommended plate	Solid black

Telephone
Fax
Email	sales@aatbio.com
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Custom size	Inquire
Technical support	Contact us
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Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on October 8, 2026

Component A: Amplite® Red Peroxidase Substrate	1 vial
Component B: H2O2	1 vial (3% stabilized solution, 200 μL)
Component C: Assay Buffer	1 bottle (100 mL)
Component D: Horseradish Peroxidase	1 vial (20 units)
Component E: DMSO	1 vial (0.5 mL)