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Amplite® Fluorimetric Sphingomyelin Assay Kit
Red Fluorescence
Sphingomyelin (SM) is largely found in the exoplasmic leaflet of the cell membrane, primarily in nervous tissue. It plays an important role in signal transduction. It accumulates abnormally in Niemann-Pick disease and Abetalipoproteinemia. Our Amplite® Fluorimetric Sphingomyelin Assay Kit provides the most sensitive method for detecting neutral SM activity or screening its inhibitors. The kit uses Amplite® Red as a fluorogenic probe to indirectly quantify the phosphocholine produced from the hydrolysis of sphingomyelin (SM) by sphingomyelinase (SMase). It can be used for measuring the SM in blood, cell extracts or other solutions. The fluorescence intensity of Amplite® Red is proportional to the formation of phosphocholine, therefore to the amount of SM. Amplite® Red enables the assay readable either in fluorescence intensity or absorption mode. The kit is an optimized "mix and read" assay that can be performed in a convenient 96-well or 384-well microtiter-plate format and easily adapted to automation without a separation step.
Sphingomyelin dose response was measured on a solid black 96-well plate with Amplite® Fluorimetric Sphingomyelin Assay Kit using a Gemini fluorescence microplate reader (Molecular Devices).
Sphingomyelin dose response was measured on a solid black 96-well plate with Amplite® Fluorimetric Sphingomyelin Assay Kit using a Gemini fluorescence microplate reader (Molecular Devices).
CatalogSize
Price
Quantity
13625100 Tests
Price
 
Spectral properties

Excitation (nm)571
Emission (nm)584
Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12352200
Instrument settings

Fluorescence microplate reader
Excitation540 nm
Emission590 nm
Cutoff570 nm
Recommended plateSolid black
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Page updated on September 25, 2025