Amplite® Universal Fluorimetric MMP Activity Assay Kit *Red Fluorescence*

Amplite® Universal Fluorimetric MMP Activity Assay Kit

Red Fluorescence

Amplite® Universal Fluorimetric MMP Activity Assay Kit *Red Fluorescence* is a fluorimetric assay kit for detection of general matrix metalloproteinase activity in biological samples.

Detection Principle: Uses MMP Red™ substrate to generate increased red fluorescence upon MMP activity.
Sensitivity: Detects sub-nanogram amounts of MMPs under AAT’s demonstrated assay conditions.
Specificity: Measures general MMP activity rather than a single MMP subtype.
Instrument Compatibility: Fluorescence detection at Ex/Em 540/590 nm using solid black microplates.

Detection of MMPs activity using Amplite® Universal Fluorimetric MMP Activity Assay Kit. The fluorescence signal was monitored one hour after the start of the reaction. The reading from all wells was subtracted with the reading from substrate control, which contains MMP Red™ substrate but no MMPs. The MMP Red™ substrate can detect the activity of sub-nanogram of all MMPs (n=3).

Protocol

SDS

Catalog	Size	Price	Quantity
13511	100 Tests	Price

Overview

The matrix metalloproteinases (MMPs) constitute a family of zinc-dependent endopeptidases that function within the extracellular matrix. These enzymes are responsible for the breakdown of connective tissues and are important in bone remodeling, the menstrual cycle and repair of tissue damage. While the exact contribution of MMPs to certain pathological processes is difficult to assess, MMPs appear to play a key role in the development of arthritis as well as in the invasion and metastasis of cancer. MMPs tend to have multiple substrates, with most family members having the ability to degrade different types of collagen along with elastin, gelatin and fibronectin. It is quite difficult to find a substrate that is selective to a single MMP enzyme. This kit is designed to check the general activity of a MMP enzyme. It can also be used to screening MMP inhibitors when a purified MMP enzyme is used. We also offer a few MMP enzyme of high activity.

Citations

View all 16 citations: Citation Explorer

MiRNA29a-3p Negatively Regulates ISL1--Integrin $\beta$1 Axis to Suppress Gastric Cancer Progression

Authors:

She, Ziwei and Dong, Haosheng and Li, Yang and Chen, Ping and Zhou, Chunyan and Wang, Weiping and Jia, Zhuqing and Shi, Qiong

Journal:

Experimental Cell Research (2024): 114288

Targeting cancer-derived extracellular vesicles by combining CD147 inhibition with tissue factor pathway inhibitor for the management of urothelial cancer cells

Authors:

Boddu, Vijay Kumar and Zamzow, Piet and Kramer, Mario Wolfgang and Merseburger, Axel S and Gorantla, Sivahari Prasad and Klinger, Matthias and Cramer, Lena and Sauer, Thorben and Gemoll, Timo and von Bubnoff, Nikolas and others,

Journal:

Cell Communication and Signaling (2024): 1--14

RNA-binding protein-regulated fibronectin is essential for EGFR-activated metastasis of head and neck squamous cell carcinoma

Authors:

Shieh, Jiunn-Min and Chang, Ting-Wei and Wang, Jing-He and Liang, Song-Ping and Kao, Pei-Lu and Chen, Liang-Yi and Yen, Chia-Jui and Chen, Yun-Ju and Chang, Wen-Chang and Chen, Ben-Kuen

Journal:

The FASEB Journal (2023): e23206

Influence of Dinitrosyl Iron Complexes on the Activity of Enzymes, Indicators of Cardiovascular Diseases

Authors:

Akentieva, Natalia and Sanina, Natalia and Gizatullin, Artur and Shkondina, Natalia and Andreeva, Anna and Shram, Stanislav and Aldoshin, Sergei

Zinc ions regulate opening of tight junction favouring efflux of macromolecules via the GSK3β/snail-mediated pathway

Authors:

Xiao, Ruyue and Yuan, Lan and He, Weijiang and Yang, Xiaoda

Journal:

Metallomics (2018)

Spectral properties

Excitation (nm)	545
Emission (nm)	572

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Fluorescence microplate reader
Excitation	540 nm
Emission	590 nm
Cutoff	570 nm
Recommended plate	Solid black

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Page updated on October 10, 2026

Component A: MMP Red™ Substrate	1 vial (60 µL)
Component B: APMA	1 vial (20 µL, 1 M)
Component C: Assay Buffer	1 bottle (20 mL)