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Anti-Procalcitonin (PCT) antibody *Mouse anti-human, monoclonal IgG1*
Ordering information
| Price | |
| Catalog Number | |
| Unit Size | |
| Quantity |
Additional ordering information
| Telephone | 1-800-990-8053 |
| Fax | 1-800-609-2943 |
| sales@aatbio.com | |
| International | See distributors |
| Bulk request | Inquire |
| Custom size | Inquire |
| Shipping | Standard overnight for United States, inquire for international |
Physical properties
| Solvent | Water |
Storage, safety and handling
| Intended use | Research Use Only (RUO) |
| Storage | Freeze (< -15 °C) |
| Overview |  SDSProtocol |
See also: Antibodies and Proteomics
Other names | Host mouse | Reactivity human | Application ELISA; CLIA |
Procalcitonin (PCT) is a peptide precursor of calcitonin and is composed of 116 amino acids. It is produced by the C cells of the thyroid and by the neuroendocrine cells of the lung and the intestine. Under normal conditions, the level of PCT in the blood of healthy individuals is very low (< .05 ng/mL) with a half-life of 25 to 30 hours in serum. In response to a pro-inflammatory stimulus, such as a pathogen, the level of PCT may increase as high as 100 μg/mL making it a suitable biomarker of bacterial infection. In clinical biology, PCT has shown to be a useful tool in the diagnosis of sepsis. Anti-PCT monoclonal antibodies are highly specific for the detection of human procalcitonin. They can be used for a broad range of immunoassays such as chemiluminescent immunoassays (CLIA).
Conjugation
We provide custom conjugation services for this antibody (eg. labeling of Anti-Procalcitonin (PCT) antibody *Mouse anti-human, monoclonal IgG1* with HRP). A list of available labels can be found in the table below:
For additional information about custom conjugations, please consult our services page here.
| AF | AF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750 |
| Proteins | HRP, Alkaline Phosphatase, Streptavidin |
| Tandems | APC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5 |
| Small Molecules | Biotin |
| Traditional Dyes | FITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5 |
| iFluor | 350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7 |
| mFluor | UV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780 |
For additional information about custom conjugations, please consult our services page here.
Images
Figure 1. Clinical comparison of AAT Bioquest’s PCT immunoassays and VIDAS® B.R.A.H.M.S PCT assay: 70 clinical blood samples were separately tested using four MAb pairs on AAT Bioquest’s CLIA platform and compared to a diagnostic kit from bioMerieux. Data from this study were analyzed using the Passing-Bablok regression method and are summarized in the following scatter plot. Results reveal good agreement between AAT Bioquest’s immunoassay and comparison assay. The Mab pairs used are (capture-detection): Mab pair A: Cat# V100120 (Clone 3) - Cat# V100120 (Clone 2); Mab pair B: Cat# V100120 (Clone 4) - Cat# V100120 (Clone 2); Mab pair C: Cat# V100120 (Clone 3) - Cat# V100120 (Clone 5); and Mab pair D: Cat# V100120 (Clone 3) - Cat# V100120 (Clone 6).
Figure 2. Calibration curve for procalcitonin in sandwich chemiluminescence immunoassay (Six calibrator concentration: 0, 0.1, 0.5, 2, 10, 50 ng/mL). All monoclonal antibodies were tested in pairs as capture and detection antibodies to determine the best two-site MAb pairs for the development of quantitative sandwich immunoassays. Detection antibodies were labeled with horseradish peroxidase (HRP). The best selected antibody pairs for quantification of human procalcitonin are (capture-detection): Mab pair A: Cat# V100115 (Clone 3) - Cat# V100115 (Clone 2); Mab pair B: Cat# V100115 (Clone 4) - Cat# V100115 (Clone 2); Mab pair C: Cat# V100115 (Clone 3) - Cat# V100115 (Clone 5); and Mab pair D: Cat# V100115 (Clone 3) - Cat# V100115 (Clone 6).
Application notes
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What is the difference between FluoroQuest Anti-fading Kit I and FluoroQuest Anti-fading Kit II?