APC Anti-human CD47 Antibody *B6.H12*

B6.H12 is an anti-human monoclonal antibody that is specific for the CD47 antigen. CD47 (sometimes called MER6, Integrin associated protein or integrin-associated protein) is a 42 - 52 kD multi-pass membrane protein that is located on the surface of cells like T cells, erythrocytes, epithelial cells and endothelial cells. CD47 acts in essential cellular pathways, for instance, the negative regulation of Fc-gamma receptor signaling pathway involved in phagocytosis and integrin-mediated signaling pathway. Also, in some organisms, it is involved in the positive regulation of stress fiber assembly, is a promoter of T cell activation and positively regulates phagocytosis. From a research standpoint, it is of biological interest due to its association with vital macromolecules/ligands like CD61. CD47 is a fairly uncommon antibody target, with a little more than 5000 publications in the last decade. Even still, CD47 is frequently used in flow cytometry applications as a phenotypic marker for differentiation of cell types, particularly in the study of immunology. This antibody was purified through affinity chromatography and conjugated to APC (ex/em = 651/660 nm). It is compatible with the 642 nm laser and 664/20 nm bandpass filter (for example, as in the Luminex Guava easyCyte).