Name: APC-iFluor® 750 Tandem
Brand: AAT Bioquest
SKU: 2626
Price: 237 USD
Availability: InStock

APC-iFluor® 750 Tandem

APC-iFluor® 750 Tandem is an APC-based near-infrared fluorescent tandem dye with improved FRET efficiency and fluorescence signal for multicolor flow cytometry.

APC-based Tandem Dye: Uses allophycocyanin as the donor fluorophore with iFluor® 750 as the acceptor dye.
Red Laser Excitation: Primary excitation at 651 nm supports red laser-compatible flow cytometry.
Near-infrared Emission: Provides fluorescence emission at 783 nm for multicolor flow cytometry.
Improved Performance: Enhanced FRET efficiency and fluorescence signal compared to APC-Cy7.
Applications: Demonstrated in spectral flow cytometry using CD3- and CD4-labeled cells.

Flow cytometry analysis of whole blood cells stained with APC/iFluor® 750 anti-human CD4 antibody (Clone: SK3). The fluorescence signal was monitored using an Aurora spectral flow cytometer in the APC/iFluor® 750 specific R7-A channel.

Datasheet

SDS

Catalog	Size	Price	Quantity
2626	1 mg	Price

Overview

Tandem dyes are a unique class of fluorescent molecules that consist of two different covalently linked fluorophores, a donor (e.g. PE or APC) and a longer-wavelength emitting fluorescence acceptor (e.g. Texas Red, Cy5, Cy7, iFluor® 594 or iFluor® 750). APC-iFluor® 750 tandem is an excellent replacement to the commonly used APC-Cy7 with improved FRET efficiency and signal. Its primary absorption peak is at 651 nm with emission peak at ~780 nm. AAT Bioquest also offers its preactivated APC-iFluor® 750 tandem to facilitate the APC-iFluor® 750 tandem conjugations to antibodies and other proteins such as streptavidin and other secondary reagents. Our preactivated APC-iFluor® 750 is ready to conjugate, giving much higher yield than the conventionally tedious SMCC-based conjugation chemistry. In addition, our preactivated APC-iFluor® 750 tandem is conjugated to a protein via its amino group that is abundant in proteins while SMCC chemistry targets the thiol group that must be regenerated by the reduction of antibodies.

References

View all 17 references: Citation Explorer

CD4+ T cells and natural killer cells: Biomarkers for hepatic fibrosis in human immunodeficiency virus/hepatitis C virus-coinfected patients.

Authors:

Laufer, Natalia and Ojeda, Diego and Polo, María Laura and Martinez, Ana and Pérez, Héctor and Turk, Gabriela and Cahn, Pedro and Zwirner, Norberto Walter and Quarleri, Jorge

Journal:

World journal of hepatology (2017): 1073-1080

Quantification of mitochondrial reactive oxygen species in living cells by using multi-laser polychromatic flow cytometry.

Authors:

De Biasi, Sara and Gibellini, Lara and Bianchini, Elena and Nasi, Milena and Pinti, Marcello and Salvioli, Stefano and Cossarizza, Andrea

Journal:

Cytometry. Part A : the journal of the International Society for Analytical Cytology (2016): 1106-1110

Presence of CD34(+)CD38(-)CD58(-) leukemia-propagating cells at diagnosis identifies patients at high risk of relapse with Ph chromosome-positive ALL after allo-hematopoietic SCT.

Authors:

Kong, Y and Xu, L-P and Liu, Y-R and Qin, Y-Z and Sun, Y-Q and Wang, Y and Jiang, H and Jiang, Q and Chen, H and Chang, Y-J and Huang, X-J

Journal:

Bone marrow transplantation (2015): 348-53

Analysis of Populations of Memory T-Helper Cells Expressing CXCR3 and CCR6 Chemokine Receptors in Peripheral Blood of Patients with Chronic Viral Hepatitis C.

Authors:

Elezov, D S and Kudryavtsev, I V and Arsent'ev, N A and Basin, V V and Esaulenko, E V and Semenov, A V and Totolyan, A A

Journal:

Bulletin of experimental biology and medicine (2015): 238-42

A flow cytometric method for the analysis of macrophages in the vascular wall.

Authors:

Moore, Jeffrey P and Sakkal, Samy and Bullen, Michelle L and Kemp-Harper, Barbara K and Ricardo, Sharon D and Sobey, Christopher G and Drummond, Grant R

Journal:

Journal of immunological methods (2013): 33-43

Physical properties

Molecular weight	~105000
Solvent	Water

Spectral properties

Absorbance (nm)	754
Extinction coefficient (cm ^-1 M ^-1)	700000
Excitation (nm)	651
Emission (nm)	783

Usage and storage

Intended use	Research Use Only (RUO)
Storage	Refrigerated (2-8 °C); Minimize light exposure

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Page updated on October 25, 2026