Name: ReadiUse™ Preactivated APC-iFluor® 750 Tandem
Brand: AAT Bioquest
SKU: 2571
Price: 406 USD
Availability: InStock

ReadiUse™ Preactivated APC-iFluor® 750 Tandem

Allophycocyanin (APC) is a phycobiliprotein isolated from Spirulina sp., a blue-green alga. Like other phycobiliproteins, APC is fluorescent, with an extremely high absorptivity and a high quantum efficiency. Our APC-iFluor® 750 Tandem is an excellent replacement for APC-Cy7® and APC-Alexa Fluor® 750 Tandem due to their similar spectral properties. Our APC-iFluor® 750 Tandem is more photostable than APC-Cy7® Tandem. ReadiUse™ Preactivated APC-iFluor® 750 Tandem is an activated APC protein, and can be easily conjugated to antibodies with much higher conjugation yield than the conventional APC.

Flow cytometry analysis of whole blood cells stained with APC/iFluor® 750 anti-human CD4 antibody (Clone: SK3). The fluorescence signal was monitored using an Aurora spectral flow cytometer in the APC/iFluor® 750 specific R7-A channel.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
2571	1 mg	Price

References

View all 46 references: Citation Explorer

Chromophore attachment to phycobiliprotein beta-subunits: phycocyanobilin:cysteine-beta84 phycobiliprotein lyase activity of CpeS-like protein from Anabaena Sp. PCC7120

Authors:

Zhao KH, Su P, Li J, Tu JM, Zhou M, Bubenzer C, Scheer H.

Journal:

J Biol Chem (2006): 8573

Excitation energy transfer from phycobiliprotein to chlorophyll d in intact cells of Acaryochloris marina studied by time- and wavelength-resolved fluorescence spectroscopy

Authors:

Petrasek Z, Schmitt FJ, Theiss C, Huyer J, Chen M, Larkum A, Eichler HJ, Kemnitz K, Eckert HJ.

Journal:

Photochem Photobiol Sci (2005): 1016

Single-molecule spectroscopy selectively probes donor and acceptor chromophores in the phycobiliprotein allophycocyanin

Authors:

Loos D, Cotlet M, De Schryver F, Habuchi S, Hofkens J.

Journal:

Biophys J (2004): 2598

Isolation and characterisation of phycobiliprotein rich mutant of cyanobacterium Synechocystis sp

Authors:

Prasanna R, Dhar DW, Dominic TK, Tiwari ON, Singh PK.

Journal:

Acta Biol Hung (2003): 113

Evaluation of Tolypothrix germplasm for phycobiliprotein content

Authors:

Prasanna R, Prasanna BM, Mohammadi SA, Singh PK.

Journal:

Folia Microbiol (Praha) (2003): 59

Physical properties

Molecular weight	~105000
Solvent	Water

Spectral properties

Extinction coefficient (cm ^-1 M ^-1)	700000
Excitation (nm)	651
Emission (nm)	791

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Refrigerated (2-8 °C); Minimize light exposure
UNSPSC	12171501

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Page updated on October 8, 2025

A: ReadiUse™ Preactivated APC-iFluor™ 750 Tandem	1 vial (1 mg)
B: Buccutite™ MTA	1 vial (100 µg)
C: Spin Desalting Column	Not Included