Name: ReadiUse™ Preactivated APC-iFluor® 750 Tandem
Brand: AAT Bioquest
SKU: 2571
Price: 415 USD
Availability: InStock

ReadiUse™ Preactivated APC-iFluor® 750 Tandem

ReadiUse™ Preactivated APC-iFluor® 750 Tandem is a ready-to-conjugate fluorescent tandem dye for labeling antibodies and other proteins for multicolor and spectral flow cytometry.

Fluorescence Detection: Excitation at 651 nm and emission at 783 nm.
Conjugation Chemistry: Buccutite™ FOL/MTA chemistry enables protein conjugation through amino-group modification.
High Conjugation Yield: Higher yields than conventional SMCC-based chemistry.
Ready-to-Use: Includes preactivated dye and Buccutite™ MTA reagent.
Improved Photostability: Greater photostability than APC-Cy7 tandem.

Flow cytometry analysis of whole blood cells stained with APC/iFluor® 750 anti-human CD4 antibody (Clone: SK3). The fluorescence signal was monitored using an Aurora spectral flow cytometer in the APC/iFluor® 750 specific R7-A channel.

Protocol

SDS

Catalog	Size	Price	Quantity
2571	1 mg	Price

Overview

Allophycocyanin (APC) is a phycobiliprotein isolated from Spirulina sp., a blue-green alga. Like other phycobiliproteins, APC is fluorescent, with an extremely high absorptivity and a high quantum efficiency. Our APC-iFluor® 750 Tandem is an excellent replacement for APC-Cy7® and APC-Alexa Fluor® 750 Tandem due to their similar spectral properties. Our APC-iFluor® 750 Tandem is more photostable than APC-Cy7® Tandem. ReadiUse™ Preactivated APC-iFluor® 750 Tandem is an activated APC protein, and can be easily conjugated to antibodies with much higher conjugation yield than the conventional APC.

References

View all 46 references: Citation Explorer

Chromophore attachment to phycobiliprotein beta-subunits: phycocyanobilin:cysteine-beta84 phycobiliprotein lyase activity of CpeS-like protein from Anabaena Sp. PCC7120

Authors:

Zhao KH, Su P, Li J, Tu JM, Zhou M, Bubenzer C, Scheer H.

Journal:

J Biol Chem (2006): 8573

Excitation energy transfer from phycobiliprotein to chlorophyll d in intact cells of Acaryochloris marina studied by time- and wavelength-resolved fluorescence spectroscopy

Authors:

Petrasek Z, Schmitt FJ, Theiss C, Huyer J, Chen M, Larkum A, Eichler HJ, Kemnitz K, Eckert HJ.

Journal:

Photochem Photobiol Sci (2005): 1016

Single-molecule spectroscopy selectively probes donor and acceptor chromophores in the phycobiliprotein allophycocyanin

Authors:

Loos D, Cotlet M, De Schryver F, Habuchi S, Hofkens J.

Journal:

Biophys J (2004): 2598

Isolation and characterisation of phycobiliprotein rich mutant of cyanobacterium Synechocystis sp

Authors:

Prasanna R, Dhar DW, Dominic TK, Tiwari ON, Singh PK.

Journal:

Acta Biol Hung (2003): 113

Evaluation of Tolypothrix germplasm for phycobiliprotein content

Authors:

Prasanna R, Prasanna BM, Mohammadi SA, Singh PK.

Journal:

Folia Microbiol (Praha) (2003): 59

Spectral properties

Extinction coefficient (cm ^-1 M ^-1)	700000
Excitation (nm)	651
Emission (nm)	783

Usage and storage

Intended use

Research Use Only (RUO)

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on October 28, 2026

Component A: ReadiUse™ Preactivated APC-iFluor® 750 Tandem	1 vial (1 mg)
Component B: Buccutite™ MTA	1 vial (100 μg)