Actively helping customers, employees and the global community during the coronavirus SARS-CoV-2 outbreak.  Learn more >>

ReadiUse™ Preactivated APC-iFluor® 700 Tandem

Our preactivated APC-iFluor® 700 Tandem was premodified with our Buccutite™ FOL (provided). Your antibody (or other proteins) is modified with our Buccutite™ MTA (provided as free sample) to give MTA-modified protein (such as antibody). The MTA-modified protein readily reacts with FOL-modified APC-iFluor® 700 Tandem (provided) to give the desired APC-iFluor® 700 Tandem-antibody conjugate in much higher yield than the SMCC chemistry. In addition our preactivated APC-iFluor® 700 Tandem reacts with MTA-modified biopolymers at much lower concentrations than the SMCC chemistry.
Our preactivated APC-iFluor® 700 Tandem was premodified with our Buccutite™ FOL (provided). Your antibody (or other proteins) is modified with our Buccutite™ MTA (provided as free sample) to give MTA-modified protein (such as antibody). The MTA-modified protein readily reacts with FOL-modified APC-iFluor® 700 Tandem (provided) to give the desired APC-iFluor® 700 Tandem-antibody conjugate in much higher yield than the SMCC chemistry. In addition our preactivated APC-iFluor® 700 Tandem reacts with MTA-modified biopolymers at much lower concentrations than the SMCC chemistry.
Ordering information
Price ()
Catalog Number2570
Unit Size
Find Distributor
Additional ordering information
Telephone1-408-733-1055
Fax1-408-733-1304
Emailsales@aatbio.com
InternationalSee distributors
ShippingStandard overnight for United States, inquire for international
Spectral properties
Extinction coefficient (cm -1 M -1)700000
Excitation (nm)651
Emission (nm)710
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12171501
Related products
ReadiUse™ Preactivated PE-iFluor® 647 Tandem
ReadiUse™ Preactivated PE-iFluor® 750 Tandem
ReadiUse™ ABTS Substrate Solution *Optimized for ELISA Assays with HRP Conjugates*
ReadiUse™ TMB Substrate Solution *Optimized for ELISA Assays with HRP Conjugates*
ReadiUse™ hydrogen peroxide solution *50 mM calibrated and stabilized solution*
ReadiUse™ Preactivated HRP NHS ester
ReadiUse™ NADPH Regenerating Kit
ReadiUse™ NADP Regenerating Kit
ReadiUse™ TCA Deproteinization Sample Preparation Kit
ReadiUse™ microscope mounting solution
ReadiUse™ 4% formaldehyde fixation solution
ReadiUse™ mammalian cell lysis buffer *5X*
ReadiUse™ probenecid, sodium salt *Water-soluble*
ReadiUse™ probenecid *25 mM stabilized aqueous solution*
ReadiUse™ CFSE [5-(and 6)-Carboxyfluorescein diacetate, succinimidyl ester] *CAS 150347-59-4*
ReadiUse™ Preactivated PE
ReadiUse™ Preactivated APC
ReadiUse™ Preactivated PE-Cy5 Tandem
ReadiUse™ Preactivated PE-Cy5.5 Tandem
ReadiUse™ Preactivated PE-Cy7 Tandem
ReadiUse™ Preactivated PE-Texas Red Tandem
ReadiUse™ Preactivated APC-Cy5.5 Tandem
ReadiUse™ Preactivated APC-Cy7 Tandem
ReadiUse™ TCEP removal solution
ReadiUse™ WST-8 *50 mM aqueous solution*
ReadiUse™ Rapid Luminometric ATP Assay Kit
ReadiUse™ PE [R-Phycoerythrin] *Ammonium Sulfate-Free*
ReadiUse™ CL-APC [Cross linked-Allophycocyanin] *Ammonium Sulfate-Free*
ReadiUse™ Preactivated PE-iFluor® 594 Tandem
ReadiUse™ Cell Detaching Buffer
ReadiUse™ DRAQ5 Staining Solution *5 mM in Water*
ReadiUse™ Staurosporine * 1 mM DMSO stock solution*
ReadiUse™ Bio-Gel P-6 spin column
ReadiUse™ bacterial cell lysis buffer *5X*
ReadiUse™ 10% Triton X-100 *Hydrogen Peroxide-and Carbonyl-Free*
ReadiUse™ dNTP Mix *10 mM*
ReadiUse™ Viral RNA Lysis Buffer
ReadiUse™ Tyramide (TSA)/Styramide (PSA) Optimized Reaction Buffer
ReadiUse™ Preactivated PerCP
ReadiUse™ Preactivated PerCP-Cy5.5 Tandem
ReadiUse™ Preactivated PE-iFluor® 660 Tandem
ReadiUse™ 10KD Spin Filter
ReadiUse™ 1 Kb Plus DNA Ladder
ReadiUse™ GeneRuler 1 kb DNA Ladder
ReadiUse™ 100 bp DNA Ladder
ReadiUse™ ddNTP Terminator Mix *10 mM*
ReadiUse™ dNTP Mix Set *10 mM PCR Grade*
ReadiUse™ Preactivated HRP maleimide
ReadiUse™ Stayright™ Purple *HRP Chromogen Premixed with Hydrogen Peroxide*
ReadiUse™ Disposable PD-10 Desalting Column
ReadiUse™ Preactivated APC Maleimide [Activated Allophycocyanin]
ReadiUse™ Preactivated PE Maleimide [Activated R-Phycoerythrin]
ReadiUse™ 6-Color Human TBNK Antibody Kit *Dry Reagent Format*
ReadiUse™ MTS Reagent *50 mM aqueous solution*
ReadiUse™ Preactivated PE-Texas Red Maleimide
ReadiUse™ Preactivated PE-Cy5 Maleimide
ReadiUse™ Preactivated PE-Cy5.5 Maleimide
ReadiUse™ Preactivated PE-Cy7 Maleimide
ReadiUse™ Preactivated APC-AF700 Maleimide
ReadiUse™ Preactivated APC-Cy5.5 Maleimide
ReadiUse™ Preactivated APC-Cy7 Maleimide
ReadiUse™ Preactivated PerCP Maleimide
ReadiUse™ Preactivated PerCP-Cy5.5 Maleimide
ReadiUse™ Preactivated PerCP-Cy7 Maleimide
Show More (64)

OverviewpdfSDSpdfProtocol


Extinction coefficient (cm -1 M -1)
700000
Excitation (nm)
651
Emission (nm)
710
Allophycocyanin (APC) is a phycobiliprotein isolated from Spirulina sp., a blue-green alga. Like other phycobiliproteins, APC is fluorescent, with an extremely high absorptivity and a high quantum efficiency. It is a protein which can be easily linked to antibodies and other proteins by conventional protein cross-linking techniques without altering its spectral characteristics. Our APC-iFluor® 700 Tandem is an excellent replacement for APC-Alexa Fluor® 700 Tandem since they have almost identical spectra. On some antibodies, our APC-iFluor® 700 Tandem is much brighter than APC-Alexa Fluor® 700 Tandem with a higher stain index. ReadiUse™ Preactivated APC-iFluor® 700 Tandem is an activated APC protein, and can be easily conjugated to antibodies with much higher conjugation yield than the conventional APC.

Components


A: ReadiUse™ Preactivated APC-iFluor™ 700 Tandem1 vial (1 mg)
B: Buccutite™ MTA1 vial (100 µg)
C: Spin Desalting ColumnNot Included

Example protocol


SAMPLE EXPERIMENTAL PROTOCOL

Preparation of pre-activated antibody with Buccutite™ MTA
  1. Reconstitute Buccutite™ MTA in DMSO at ~10 mg/mL.
    Note     Please store unused Buccutite™ MTA at -20 °C and could be used up to two freeze and thaw cycles.
  2. Prepare target antibody (Ab) in pH = 8.5 - 9.0 buffer at concentration above 1 mg/mL.
  3. Add Buccutite™ MTA to Ab solution at the ratio of 8 - 10 µg Buccutite™ MTA/100 µg Ab.
  4. Mix well and react at room temperature for 60 minutes, rotating during the reaction.
  5. Purify the reaction mixture with desalting column to remove unreacted Buccutite™ MTA and exchange buffer to PBS or buffer of your choice.
  6. Collect the Buccutite™ MTA-activated Ab, and estimate the concentration by 70% yield of the original starting amount. 

Conjugation with pre-activated APC-iFluor™ 700 Tandem
  1. Reconstitute pre-activated APC-iFluor™ 700 Tandem in 100 µL ddH2O to 10 mg/mL.
    Note     Reconstituted pre-activated APC-iFluor™ 700 Tandem could be stored at 4 °C for one month, kept from light.
  2. Add APC-iFluor™ 700 Tandem directly to MTA-activated target Ab solution at the ratio of 130 µg APC-iFluor™ 700 Tandem/100 µg MTA-activated Ab.
  3. Rotate the mixture for 60 minutes at room temperature.
  4. The Ab/APC-iFluor™ 700 Tandem conjugates are now ready to use.
    Note      The antibody conjugate should be stored at > 0.5 mg/mL in the presence of a carrier protein (e.g., 0.1% bovine serum albumin) and 0.02% - 0.05% sodium azide. The Ab/APC-iFluor™ 700 Tandem conjugates solution could be stored at 4 °C for up to two months, and kept from light.
  5. (Optional) Ab/APC-iFluor™ 700 Tandem conjugates could be further purified through size exclusion chromatography to get best performance. 

Spectrum


Open in Advanced Spectrum Viewer
spectrum

Spectral properties

Extinction coefficient (cm -1 M -1)700000
Excitation (nm)651
Emission (nm)710

Product family


NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)
ReadiUse™ Preactivated APC-iFluor® 750 Tandem754793700000
ReadiUse™ Preactivated APC-iFluor® 800 Tandem804660700000
ReadiUse™ Preactivated PE-iFluor® 700 Tandem5667081960000

References


View all 46 references: Citation Explorer
Chromophore attachment to phycobiliprotein beta-subunits: phycocyanobilin:cysteine-beta84 phycobiliprotein lyase activity of CpeS-like protein from Anabaena Sp. PCC7120
Authors: Zhao KH, Su P, Li J, Tu JM, Zhou M, Bubenzer C, Scheer H.
Journal: J Biol Chem (2006): 8573
Excitation energy transfer from phycobiliprotein to chlorophyll d in intact cells of Acaryochloris marina studied by time- and wavelength-resolved fluorescence spectroscopy
Authors: Petrasek Z, Schmitt FJ, Theiss C, Huyer J, Chen M, Larkum A, Eichler HJ, Kemnitz K, Eckert HJ.
Journal: Photochem Photobiol Sci (2005): 1016
Single-molecule spectroscopy selectively probes donor and acceptor chromophores in the phycobiliprotein allophycocyanin
Authors: Loos D, Cotlet M, De Schryver F, Habuchi S, Hofkens J.
Journal: Biophys J (2004): 2598
Isolation and characterisation of phycobiliprotein rich mutant of cyanobacterium Synechocystis sp
Authors: Prasanna R, Dhar DW, Dominic TK, Tiwari ON, Singh PK.
Journal: Acta Biol Hung (2003): 113
Evaluation of Tolypothrix germplasm for phycobiliprotein content
Authors: Prasanna R, Prasanna BM, Mohammadi SA, Singh PK.
Journal: Folia Microbiol (Praha) (2003): 59
Co-ordinated expression of phycobiliprotein operons in the chromatically adapting cyanobacterium Calothrix PCC 7601: a role for RcaD and RcaG
Authors: Noubir S, Luque I, Ochoa de Alda JA, Perewoska I, T and eau de Marsac N, Cobley JG, Houmard J.
Journal: Mol Microbiol (2002): 749
Phycobiliprotein genes of the marine photosynthetic prokaryote Prochlorococcus: evidence for rapid evolution of genetic heterogeneity
Authors: Ting CS, Rocap G, King J, Chisholm SW.
Journal: Microbiology (2001): 3171
Phycobiliprotein-Fab conjugates as probes for single particle fluorescence imaging
Authors: Triantafilou K, Triantafilou M, Wilson KM.
Journal: Cytometry (2000): 226
Novel activity of a phycobiliprotein lyase: both the attachment of phycocyanobilin and the isomerization to phycoviolobilin are catalyzed by the proteins PecE and PecF encoded by the phycoerythrocyanin operon
Authors: Zhao KH, Deng MG, Zheng M, Zhou M, Parbel A, Storf M, Meyer M, Strohmann B, Scheer H.
Journal: FEBS Lett (2000): 9
Phycobiliprotein and fluorescence immunological assay
Authors: Wu P., undefined
Journal: Sheng Li Ke Xue Jin Zhan (2000): 82