ReadiUse™ Preactivated APC-iFluor™ 700 Tandem

! Interactive Product Finder: This product has alternative forms and/or upgrades. View now


Image Viewer
<p>Our preactivated APC was premodified with our Buccutite™ FOL (provided). Your antibody (or other proteins) is modified with our Buccutite™ MTA (provided as free sample) to give MTA-modified protein (such as antibody). The MTA-modified protein readily reacts with FOL-modified APC (provided) to give the desired APC-antibody conjugate in much higher yield than the SMCC chemistry. In addition our preactivated APC reacts with MTA-modified biopolymers at much lower concentrations than the SMCC chemistry.</p>
Roll over image to zoom in
Loading...
 
Unit Size: Cat No: Price (USD): Qty:
1 mg 2570 $345


Export item/cart as Excel file
Send item/cart as email
EXPORT TO EXCEL X

Export:
EXPORT TO EMAIL X
Important: We request your email address to ensure that the recipient(s) knows you intended for them to see the email, and that it is not junk mail.
Export:
Your Name*:
Your Email*:
Recipient Email*:
Your Personal Message:
Additional Ordering Information
Telephone: 1-800-990-8053
Fax: 1-408-733-1304
Email: sales@aatbio.com
International: See distributors





Overview

Ex/Em (nm)651/710
SolventWater
Storage Refrigerated (2-4 °C)
Minimize light exposure
Category Protein-Based Tags
Phycobiliproteins
Related Protein Dyes
Allophycocyanin (APC) is a phycobiliprotein isolated from Spirulina sp., a blue-green alga. Like other phycobiliproteins, APC is fluorescent, with an extremely high absorptivity and a high quantum efficiency. It is a protein which can be easily linked to antibodies and other proteins by conventional protein cross-linking techniques without altering its spectral characteristics. Our APC-iFluor™ 700 Tandem is an excellent replacement for APC-Alexa Fluor® 700 Tandem since they have almost identical spectra. On some antibodies, our APC-iFluor™ 700 Tandem is much brighter than APC-Alexa Fluor® 700 Tandem with a higher stain index. ReadiUse™ Preactivated APC-iFluor™ 700 Tandem is an activated APC protein, and can be easily conjugated to antibodies with much higher conjugation yield than the conventional APC.




Spectrum Advanced Spectrum Viewer

Sorry, your browser does not support inline SVG. Relative Intensity (%) 100 80 60 40 20 0 Sorry, your browser does not support inline SVG.
Sorry, your browser does not support inline SVG. Sorry, your browser does not support inline SVG.
Move mouse over grid to display wavelength & intensity values.

300
400
500
600
700
800
900
Wavelength (nm)





Protocol


Quick Preview

This protocol only provides a guideline, and should be modified according to your specific needs.
  1. Pre-activate antibody with Buccutite™ MTA
    1. Reconstitute Buccutite™ MTA in DMSO at ~10 mg/mL.
      Note: Please store unused Buccutite™ MTA at -20°C and could be used up to two freeze and thaw cycles
    2. Prepare target antibody (Ab) in pH = 8.5~9.0 buffer at concentration above 1mg/ml.
    3. Add Buccutite™ MTA to Ab solution at the ratio of 8~10 µg Buccutite™ MTA /100 µg Ab.
    4. Mix well and react at RT for 60 minutes, rotating during the reaction.
    5. Purify the reaction mixture with desalting column to remove unreacted Buccutite™ MTA and exchange buffer to PBS or buffer of your choice.
    6. Collect the Buccutite™ MTA-activated Ab, and estimate the concentration by 70% yield of the original starting amount.

  2. Conjugate with pre-activated APC, APC-ifluors, or APC-ifluor tandems
    1. Reconstitute pre-activated APC, APC, APC-ifluors, or APC-ifluor tandems in 100 µL ddH2O to 10mg/mL.
      Note: Reconstituted pre-activated APC, APC-ifluors, or APC-ifluor tandems are not stable and could be stored at 4 °C for one month, kept from light.
    2. Add APC, APC-ifluors, or APC-ifluor tandems directly to MTA-activated target Ab solution (from 1.5) at the ratio of 130 µg APC, APC-ifluors, or APC-ifluor tandems /100µg MTA-activated Ab.
    3. Rotate the mixture for 1 hour at room temperature
    4. The Ab/APC, APC-ifluors, or APC-ifluor tandems conjugates are now ready to use.
      Note 1(Optional): Ab/APC, APC-ifluors, or APC-ifluor tandems conjugates could be further purified through size exclusion chromatography to get best performance.
      Note 2: The antibody conjugate should be stored at > 0.5 mg/mL in the presence of a carrier protein (e.g., 0.1% bovine serum albumin) and 0.02-0.05% sodium azide.
      Note 3: The Ab/APC, APC-ifluors, or APC-ifluor tandems conjugates solution could be stored at 4 °C for up to two months, and kept from light.





Interactive Product Finder

Similar products
Product nameCurrent ProductRelated Product
ReadiUse™ Preactivated APC-iFluor™ 750 Tandem
700
750






References

Chromophore attachment to phycobiliprotein beta-subunits: phycocyanobilin:cysteine-beta84 phycobiliprotein lyase activity of CpeS-like protein from Anabaena Sp. PCC7120
Authors: Zhao KH, Su P, Li J, Tu JM, Zhou M, Bubenzer C, Scheer H.
Journal: J Biol Chem (2006): 8573

Excitation energy transfer from phycobiliprotein to chlorophyll d in intact cells of Acaryochloris marina studied by time- and wavelength-resolved fluorescence spectroscopy
Authors: Petrasek Z, Schmitt FJ, Theiss C, Huyer J, Chen M, Larkum A, Eichler HJ, Kemnitz K, Eckert HJ.
Journal: Photochem Photobiol Sci (2005): 1016

Single-molecule spectroscopy selectively probes donor and acceptor chromophores in the phycobiliprotein allophycocyanin
Authors: Loos D, Cotlet M, De Schryver F, Habuchi S, Hofkens J.
Journal: Biophys J (2004): 2598

Evaluation of Tolypothrix germplasm for phycobiliprotein content
Authors: Prasanna R, Prasanna BM, Mohammadi SA, Singh PK.
Journal: Folia Microbiol (Praha) (2003): 59

Isolation and characterisation of phycobiliprotein rich mutant of cyanobacterium Synechocystis sp
Authors: Prasanna R, Dhar DW, Dominic TK, Tiwari ON, Singh PK.
Journal: Acta Biol Hung (2003): 113

Co-ordinated expression of phycobiliprotein operons in the chromatically adapting cyanobacterium Calothrix PCC 7601: a role for RcaD and RcaG
Authors: Noubir S, Luque I, Ochoa de Alda JA, Perewoska I, Tandeau de Marsac N, Cobley JG, Houmard J.
Journal: Mol Microbiol (2002): 749

Phycobiliprotein genes of the marine photosynthetic prokaryote Prochlorococcus: evidence for rapid evolution of genetic heterogeneity
Authors: Ting CS, Rocap G, King J, Chisholm SW.
Journal: Microbiology (2001): 3171

Novel activity of a phycobiliprotein lyase: both the attachment of phycocyanobilin and the isomerization to phycoviolobilin are catalyzed by the proteins PecE and PecF encoded by the phycoerythrocyanin operon
Authors: Zhao KH, Deng MG, Zheng M, Zhou M, Parbel A, Storf M, Meyer M, Strohmann B, Scheer H.
Journal: FEBS Lett (2000): 9

Phycobiliprotein-Fab conjugates as probes for single particle fluorescence imaging
Authors: Triantafilou K, Triantafilou M, Wilson KM.
Journal: Cytometry (2000): 226

[Phycobiliprotein and fluorescence immunological assay]
Authors: Wu P.
Journal: Sheng Li Ke Xue Jin Zhan (2000): 82
View More Citations




Documents

 
Safety Data Sheet (SDS)


Catalogs
1. Fluorescent Labeling Probes & Kits

Certificate of Analysis