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APC-iFluor®A7 Tandem

Flow cytometry analysis of whole blood stained with APC-iFluor® A7 anti-human CD3 *SK7* conjugate. The fluorescence signal was monitored using an Aurora spectral flow cytometer in the APC-iFluor® A7 specific R7-A channel.
Flow cytometry analysis of whole blood stained with APC-iFluor® A7 anti-human CD3 *SK7* conjugate. The fluorescence signal was monitored using an Aurora spectral flow cytometer in the APC-iFluor® A7 specific R7-A channel.
Flow cytometry analysis of whole blood stained with APC-iFluor® A7 anti-human CD3 *SK7* conjugate. The fluorescence signal was monitored using an Aurora spectral flow cytometer in the APC-iFluor® A7 specific R7-A channel.
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Physical properties
Molecular weightN/A
SolventWater
Spectral properties
Extinction coefficient (cm -1 M -1)700000
Excitation (nm)651
Emission (nm)782
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageRefrigerated (2-8 °C); Minimize light exposure

OverviewpdfSDSpdfProtocol


Molecular weight
N/A
Extinction coefficient (cm -1 M -1)
700000
Excitation (nm)
651
Emission (nm)
782
Tandem dyes are a unique class of fluorescent molecules that consist of two different covalently linked fluorophores, a donor (e.g. PE or APC) and a longer-wavelength emitting fluorescence acceptor (e.g. Texas Red, Cy5, Cy7, iFluor® 594 or iFluor® 750). APC-Cy7 is one of the most popular tandem colors for multicolor applications. There were also somewhat improved replacements such as APC-Alexa Fluor 750, APC-H7 and APC-Fire 750. However, the performance of APC-Cy7, APC-Alexa Fluor 750 and APC-Fire 750 is quite similar, and the claimed improvement is insignificant. Our APC-A7 is significantly brighter than APC-Cy7, APC-Alexa Fluor 750, APC-H7 and APC-Fire 750. It is a true upgrade to the existing APC-Cy7 tandems.

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Spectral properties

Extinction coefficient (cm -1 M -1)700000
Excitation (nm)651
Emission (nm)782

Product Family


NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)
APC-iFluor® 700 Tandem651710700000
APC-iFluor® 750 Tandem651776700000
APC-iFluor® 800 Tandem651819700000
APC-iFluor® 710 Tandem651747700000
APC-iFluor® 720 Tandem651748700000
APC-iFluor® 740 Tandem651764700000
APC-iFluor® 770 Tandem780800700000
APC-iFluor® 780 Tandem787812700000

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References


View all 17 references: Citation Explorer
CD4+ T cells and natural killer cells: Biomarkers for hepatic fibrosis in human immunodeficiency virus/hepatitis C virus-coinfected patients.
Authors: Laufer, Natalia and Ojeda, Diego and Polo, María Laura and Martinez, Ana and Pérez, Héctor and Turk, Gabriela and Cahn, Pedro and Zwirner, Norberto Walter and Quarleri, Jorge
Journal: World journal of hepatology (2017): 1073-1080
Quantification of mitochondrial reactive oxygen species in living cells by using multi-laser polychromatic flow cytometry.
Authors: De Biasi, Sara and Gibellini, Lara and Bianchini, Elena and Nasi, Milena and Pinti, Marcello and Salvioli, Stefano and Cossarizza, Andrea
Journal: Cytometry. Part A : the journal of the International Society for Analytical Cytology (2016): 1106-1110
Presence of CD34(+)CD38(-)CD58(-) leukemia-propagating cells at diagnosis identifies patients at high risk of relapse with Ph chromosome-positive ALL after allo-hematopoietic SCT.
Authors: Kong, Y and Xu, L-P and Liu, Y-R and Qin, Y-Z and Sun, Y-Q and Wang, Y and Jiang, H and Jiang, Q and Chen, H and Chang, Y-J and Huang, X-J
Journal: Bone marrow transplantation (2015): 348-53
Analysis of Populations of Memory T-Helper Cells Expressing CXCR3 and CCR6 Chemokine Receptors in Peripheral Blood of Patients with Chronic Viral Hepatitis C.
Authors: Elezov, D S and Kudryavtsev, I V and Arsent'ev, N A and Basin, V V and Esaulenko, E V and Semenov, A V and Totolyan, A A
Journal: Bulletin of experimental biology and medicine (2015): 238-42
A flow cytometric method for the analysis of macrophages in the vascular wall.
Authors: Moore, Jeffrey P and Sakkal, Samy and Bullen, Michelle L and Kemp-Harper, Barbara K and Ricardo, Sharon D and Sobey, Christopher G and Drummond, Grant R
Journal: Journal of immunological methods (2013): 33-43
Combined normal donor and CLL: Single tube ZAP-70 analysis.
Authors: Degheidy, Heba A and Venzon, David J and Farooqui, Mohammed Z H and Abbasi, Fatima and Arthur, Diane C and Wilson, Wyndham H and Wiestner, Adrian and Stetler-Stevenson, M A and Marti, Gerald E
Journal: Cytometry. Part B, Clinical cytometry (2012): 67-77
The role of CD19 and CD27 in the diagnosis of multiple myeloma by flow cytometry: a new statistical model.
Authors: Cannizzo, Elisa and Carulli, Giovanni and Del Vecchio, Luigi and Ottaviano, Virginia and Bellio, Emanuele and Zenari, Ezio and Azzarà, Antonio and Petrini, Mario and Preffer, Frederic
Journal: American journal of clinical pathology (2012): 377-86
Measurement conditions for flow cytometry analyses of cell lines from urological carcinomas.
Authors: Tölle, Angelika and Abdallah, Ziyad and Jung, Klaus and Bäumler, Hans
Journal: Journal of fluorescence (2010): 779-86
Flow cytometry APC-tandem dyes are degraded through a cell-dependent mechanism.
Authors: Le Roy, Christine and Varin-Blank, Nadine and Ajchenbaum-Cymbalista, Florence and Letestu, Rémi
Journal: Cytometry. Part A : the journal of the International Society for Analytical Cytology (2009): 882-90
An optimized flow cytometry protocol for analysis of angiogenic monocytes and endothelial progenitor cells in peripheral blood.
Authors: Hristov, Mihail and Schmitz, Susanne and Schuhmann, Christoph and Leyendecker, Thorsten and von Hundelshausen, Philipp and Krötz, Florian and Sohn, Hae-Young and Nauwelaers, Frans A and Weber, Christian
Journal: Cytometry. Part A : the journal of the International Society for Analytical Cytology (2009): 848-53