AzoDye-2 PEG10 Alkyne

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Physical properties

Molecular weight	984.11
Solvent	DMSO

Spectral properties

Absorbance (nm)

554

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Alternative formats

AzoDye-2-PEG10 Azide

Overview SDS Protocol

Molecular weight

984.11

Absorbance (nm)

554

AzoDye-2 PEG10 alkyne is an excellent building block for labeling azide-containing molecules. It contains a PEG10 spacer that significantly improves its water solubility compared to AzoDye-2 alkyne (#2434) although both AzoDye-2 alkyne (#2434) and AzoDye-2 PEG10 alkyne (#2435) bear the same extremely hydrophobic BHQ-2 chromophore. AzoDye-2 dyes are nearly non-fluorescent with moderate extinction coefficients. These dark quenchers are most typically used in Molecular Beacon probes in which FRET has minimal contribution to their fluorescence quenching, thus dependence on donor-acceptor spectral overlap is not significant as to other FRET probes such as FRET peptides. AzoDye-2 dyes can quench a variety of fluorophores, in particular, fluorescein derivatives such as FAM, HEX, TET, NED and JOE. This may reflect the AzoDye-2's ability to form stable, non-fluorescent complexes with a fluorophore.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of AzoDye-2 PEG10 Alkyne to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	101.615 µL	508.073 µL	1.016 mL	5.081 mL	10.161 mL
5 mM	20.323 µL	101.615 µL	203.229 µL	1.016 mL	2.032 mL
10 mM	10.161 µL	50.807 µL	101.615 µL	508.073 µL	1.016 mL

Molarity calculator

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Spectrum

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Spectral properties

Absorbance (nm)

554

Images

Figure 1. Chemical structure for AzoDye-2 PEG10 Alkyne.

References

View all 32 references: Citation Explorer

Antibody-powered DNA switches to initiate the hybridization chain reaction for the amplified fluorescence immunoassay.
Authors: He, Jia-Yang and Chen, Ze-Hui and Deng, Hui-Lin and Yuan, Ruo and Xu, Wen-Ju
Journal: The Analyst (2021): 5067-5073

Anticancer Activity Assessment and DNA Binding Properties of Two Binuclear Platinum (II) Complexes using Spectroscopic and Molecular Simulation Approaches.
Authors: Alavianmehr, Mohammad M and Ashrafi, Abolfazl and Yousefi, Reza and Haghighi, Mohsen G and Abolmaali, Samira S and Moosavi-Movahedi, Ali A and Rad, Mohammad N S
Journal: Anti-cancer agents in medicinal chemistry (2020): 2066-2073

Heck- and Suzuki-coupling approaches to novel hydroquinone inhibitors of calcium ATPase.
Authors: Kempton, Robert J and Kidd-Kautz, Taylor A and Laurenceau, Soizic and Paula, Stefan
Journal: Beilstein journal of organic chemistry (2019): 971-975

Förster Resonance Energy Transfer-Based Soft Nanoballs for Specific and Amplified Detection of MicroRNAs.
Authors: Cheng, Yun Ying and Xie, Yi Fen and Li, Chun Mei and Li, Yuan Fang and Huang, Cheng Zhi
Journal: Analytical chemistry (2019): 11023-11029

Influence of the Diphosphine Coordination Mode on the Structural and Optical Properties of Cyclometalated Platinum(II) Complexes: An Experimental and Theoretical Study on Intramolecular Pt···Pt and π···π Interactions.
Authors: Barzegar-Kiadehi, S Reza and Golbon Haghighi, Mohsen and Jamshidi, Mahboubeh and Notash, Behrouz
Journal: Inorganic chemistry (2018): 5060-5073

Efficient Synthesis of a Wide-Range Absorbing Azaphthalocyanine Dark Quencher and Its Application to Dual-Labeled Oligonucleotide Probes for Quantitative Real-Time Polymerase Chain Reactions.
Authors: Demuth, Jiri and Kucera, Radim and Kopecky, Kamil and Havlínová, Zuzana and Libra, Antonín and Novakova, Veronika and Miletin, Miroslav and Zimcik, Petr
Journal: Chemistry (Weinheim an der Bergstrasse, Germany) (2018): 9658-9666

Synthesis, structural characterization and biological properties of phosphorescent iridium(III) complexes.
Authors: Bhat, Satish S and Shivalingegowda, Naveen and Revankar, Vidyanand K and Lokanath, N K and Kugaji, Manohar S and Kumbar, Vijay and Bhat, Kishore
Journal: Journal of inorganic biochemistry (2017): 127-137

The G-BHQ synergistic effect: Improved double quenching molecular beacons based on guanine and Black Hole Quencher for sensitive simultaneous detection of two DNAs.
Authors: Xiang, Dongshan and Li, Fengquan and Wu, Chenyi and Shi, Boan and Zhai, Kun
Journal: Talanta (2017): 289-294

Silver enhanced ratiometric nanosensor based on two adjustable Fluorescence Resonance Energy Transfer modes for quantitative protein sensing.
Authors: Li, Hui and Zhao, Yaju and Chen, Zhu and Xu, Danke
Journal: Biosensors & bioelectronics (2017): 428-432

Target-triggered signal turn-on detection of prostate specific antigen based on metal-enhanced fluorescence of Ag@SiO2@SiO2-RuBpy composite nanoparticles.
Authors: Deng, Yun-Liang and Xu, Dang-Dang and Pang, Dai-Wen and Tang, Hong-Wu
Journal: Nanotechnology (2017): 065501