AzoDye-2 PEG10 Alkyne
Ordering information
Price | |
Catalog Number | |
Unit Size | |
Quantity |
Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 984.11 |
Solvent | DMSO |
Spectral properties
Absorbance (nm) | 554 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12171501 |
Alternative formats
AzoDye-2-PEG10 Azide |
Overview | ![]() ![]() |
See also: Förster Resonance Energy Transfer (FRET)
Molecular weight 984.11 | Absorbance (nm) 554 |
AzoDye-2 PEG10 alkyne is an excellent building block for labeling azide-containing molecules. It contains a PEG10 spacer that significantly improves its water solubility compared to AzoDye-2 alkyne (#2434) although both AzoDye-2 alkyne (#2434) and AzoDye-2 PEG10 alkyne (#2435) bear the same extremely hydrophobic BHQ-2 chromophore. AzoDye-2 dyes are nearly non-fluorescent with moderate extinction coefficients. These dark quenchers are most typically used in Molecular Beacon probes in which FRET has minimal contribution to their fluorescence quenching, thus dependence on donor-acceptor spectral overlap is not significant as to other FRET probes such as FRET peptides. AzoDye-2 dyes can quench a variety of fluorophores, in particular, fluorescein derivatives such as FAM, HEX, TET, NED and JOE. This may reflect the AzoDye-2's ability to form stable, non-fluorescent complexes with a fluorophore.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of AzoDye-2 PEG10 Alkyne to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 101.615 µL | 508.073 µL | 1.016 mL | 5.081 mL | 10.161 mL |
5 mM | 20.323 µL | 101.615 µL | 203.229 µL | 1.016 mL | 2.032 mL |
10 mM | 10.161 µL | 50.807 µL | 101.615 µL | 508.073 µL | 1.016 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
/ | = | x | = |
References
View all 32 references: Citation Explorer
Antibody-powered DNA switches to initiate the hybridization chain reaction for the amplified fluorescence immunoassay.
Authors: He, Jia-Yang and Chen, Ze-Hui and Deng, Hui-Lin and Yuan, Ruo and Xu, Wen-Ju
Journal: The Analyst (2021): 5067-5073
Authors: He, Jia-Yang and Chen, Ze-Hui and Deng, Hui-Lin and Yuan, Ruo and Xu, Wen-Ju
Journal: The Analyst (2021): 5067-5073
Anticancer Activity Assessment and DNA Binding Properties of Two Binuclear Platinum (II) Complexes using Spectroscopic and Molecular Simulation Approaches.
Authors: Alavianmehr, Mohammad M and Ashrafi, Abolfazl and Yousefi, Reza and Haghighi, Mohsen G and Abolmaali, Samira S and Moosavi-Movahedi, Ali A and Rad, Mohammad N S
Journal: Anti-cancer agents in medicinal chemistry (2020): 2066-2073
Authors: Alavianmehr, Mohammad M and Ashrafi, Abolfazl and Yousefi, Reza and Haghighi, Mohsen G and Abolmaali, Samira S and Moosavi-Movahedi, Ali A and Rad, Mohammad N S
Journal: Anti-cancer agents in medicinal chemistry (2020): 2066-2073
Heck- and Suzuki-coupling approaches to novel hydroquinone inhibitors of calcium ATPase.
Authors: Kempton, Robert J and Kidd-Kautz, Taylor A and Laurenceau, Soizic and Paula, Stefan
Journal: Beilstein journal of organic chemistry (2019): 971-975
Authors: Kempton, Robert J and Kidd-Kautz, Taylor A and Laurenceau, Soizic and Paula, Stefan
Journal: Beilstein journal of organic chemistry (2019): 971-975
Förster Resonance Energy Transfer-Based Soft Nanoballs for Specific and Amplified Detection of MicroRNAs.
Authors: Cheng, Yun Ying and Xie, Yi Fen and Li, Chun Mei and Li, Yuan Fang and Huang, Cheng Zhi
Journal: Analytical chemistry (2019): 11023-11029
Authors: Cheng, Yun Ying and Xie, Yi Fen and Li, Chun Mei and Li, Yuan Fang and Huang, Cheng Zhi
Journal: Analytical chemistry (2019): 11023-11029
Influence of the Diphosphine Coordination Mode on the Structural and Optical Properties of Cyclometalated Platinum(II) Complexes: An Experimental and Theoretical Study on Intramolecular Pt···Pt and π···π Interactions.
Authors: Barzegar-Kiadehi, S Reza and Golbon Haghighi, Mohsen and Jamshidi, Mahboubeh and Notash, Behrouz
Journal: Inorganic chemistry (2018): 5060-5073
Authors: Barzegar-Kiadehi, S Reza and Golbon Haghighi, Mohsen and Jamshidi, Mahboubeh and Notash, Behrouz
Journal: Inorganic chemistry (2018): 5060-5073
Efficient Synthesis of a Wide-Range Absorbing Azaphthalocyanine Dark Quencher and Its Application to Dual-Labeled Oligonucleotide Probes for Quantitative Real-Time Polymerase Chain Reactions.
Authors: Demuth, Jiri and Kucera, Radim and Kopecky, Kamil and Havlínová, Zuzana and Libra, Antonín and Novakova, Veronika and Miletin, Miroslav and Zimcik, Petr
Journal: Chemistry (Weinheim an der Bergstrasse, Germany) (2018): 9658-9666
Authors: Demuth, Jiri and Kucera, Radim and Kopecky, Kamil and Havlínová, Zuzana and Libra, Antonín and Novakova, Veronika and Miletin, Miroslav and Zimcik, Petr
Journal: Chemistry (Weinheim an der Bergstrasse, Germany) (2018): 9658-9666
Synthesis, structural characterization and biological properties of phosphorescent iridium(III) complexes.
Authors: Bhat, Satish S and Shivalingegowda, Naveen and Revankar, Vidyanand K and Lokanath, N K and Kugaji, Manohar S and Kumbar, Vijay and Bhat, Kishore
Journal: Journal of inorganic biochemistry (2017): 127-137
Authors: Bhat, Satish S and Shivalingegowda, Naveen and Revankar, Vidyanand K and Lokanath, N K and Kugaji, Manohar S and Kumbar, Vijay and Bhat, Kishore
Journal: Journal of inorganic biochemistry (2017): 127-137
The G-BHQ synergistic effect: Improved double quenching molecular beacons based on guanine and Black Hole Quencher for sensitive simultaneous detection of two DNAs.
Authors: Xiang, Dongshan and Li, Fengquan and Wu, Chenyi and Shi, Boan and Zhai, Kun
Journal: Talanta (2017): 289-294
Authors: Xiang, Dongshan and Li, Fengquan and Wu, Chenyi and Shi, Boan and Zhai, Kun
Journal: Talanta (2017): 289-294
Silver enhanced ratiometric nanosensor based on two adjustable Fluorescence Resonance Energy Transfer modes for quantitative protein sensing.
Authors: Li, Hui and Zhao, Yaju and Chen, Zhu and Xu, Danke
Journal: Biosensors & bioelectronics (2017): 428-432
Authors: Li, Hui and Zhao, Yaju and Chen, Zhu and Xu, Danke
Journal: Biosensors & bioelectronics (2017): 428-432
Target-triggered signal turn-on detection of prostate specific antigen based on metal-enhanced fluorescence of Ag@SiO2@SiO2-RuBpy composite nanoparticles.
Authors: Deng, Yun-Liang and Xu, Dang-Dang and Pang, Dai-Wen and Tang, Hong-Wu
Journal: Nanotechnology (2017): 065501
Authors: Deng, Yun-Liang and Xu, Dang-Dang and Pang, Dai-Wen and Tang, Hong-Wu
Journal: Nanotechnology (2017): 065501
Application notes
FAQ
AssayWise
A practical guide for use of PE and APC in flow cytometry
ReadiUse™ Lyophilized Phycobiliproteins
Therapeutic Monoclonal Antibody Fluorescent Labeling Tools for Imaging and Flow Cytometry
Development and Applications of Coronavirus Protease-Specific Covidyte™ and Covipyte™ Peptide Substrates
Enzymatic and Chemical Labeling Strategies for Oligonucleotides
ReadiUse™ Lyophilized Phycobiliproteins
Therapeutic Monoclonal Antibody Fluorescent Labeling Tools for Imaging and Flow Cytometry
Development and Applications of Coronavirus Protease-Specific Covidyte™ and Covipyte™ Peptide Substrates
Enzymatic and Chemical Labeling Strategies for Oligonucleotides