AzoDye-2-PEG10 Azide

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Fax	1-800-609-2943
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Physical properties

Molecular weight	1015.13
Solvent	DMSO

Spectral properties

Absorbance (nm)

554

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure

Alternative formats

AzoDye-2 PEG10 Alkyne

Overview SDS Protocol

Molecular weight

1015.13

Absorbance (nm)

554

AzoDye-2 PEG 10 azide is an excellent building block for labeling alkyne-containing molecules. It contains a PEG10 spacer to increase its water solubility. AzoDye-2 dyes are nearly non-fluorescent with moderate extinction coefficients. These dark quenchers are most typically used in Molecular Beacon probes in which FRET has minimal contribution to their fluorescence quenching, thus dependence on donor-acceptor spectral overlap is not significant as to other FRET probes such as FRET peptides. AzoDye-2 dyes can quench a variety of fluorophores, in particular, fluorescein derivatives such as FAM, HEX, TET, NED and JOE. This may reflect the AzoDye-2's ability to form stable, non-fluorescent complexes with a fluorophore.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of AzoDye-2-PEG10 Azide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	98.51 µL	492.548 µL	985.096 µL	4.925 mL	9.851 mL
5 mM	19.702 µL	98.51 µL	197.019 µL	985.096 µL	1.97 mL
10 mM	9.851 µL	49.255 µL	98.51 µL	492.548 µL	985.096 µL

Molarity calculator

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Spectrum

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Spectral properties

Absorbance (nm)

554

Images

Figure 1. AzoDye-2 PEG 10 azide is an excellent building block for labeling alkyne-containing molecules. It contains a PEG10 spacer to increase its water solubility. AzoDye-2 dyes are nearly non-fluorescent with moderate extinction coefficients.

References

View all 9 references: Citation Explorer

FRET-based hACE2 receptor mimic peptide conjugated nanoprobe for simple detection of SARS-CoV-2.
Authors: Kang, Byunghoon and Lee, Youngjin and Lim, Jaewoo and Yong, Dongeun and Ki Choi, Young and Woo Yoon, Sun and Seo, Seungbeom and Jang, Soojin and Uk Son, Seong and Kang, Taejoon and Jung, Juyeon and Lee, Kyu-Sun and Kim, Myung Hee and Lim, Eun-Kyung
Journal: Chemical engineering journal (Lausanne, Switzerland : 1996) (2022): 136143

Capability of novel fluorescence DNA-conjugated CdTe/ZnS quantum dots nanoprobe for COVID-19 sensing.
Authors: Bardajee, Ghasem Rezanejade and Zamani, Mohammadreza and Mahmoodian, Hossein and Elmizadeh, Hamideh and Yari, Hadi and Jouyandeh, Lavin and Shirkavand, Razieh and Sharifi, Mahdieh
Journal: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy (2022): 120702

Efficient and Versatile Application of Fluorescence DNA-Conjugated CdTe Quantum Dots Nanoprobe for Detection of a Specific Target DNA of SARS Cov-2 Virus.
Authors: Bardajee, Ghasem Rezanejade and Zamani, Mohammadreza and Sharifi, Mahdieh
Journal: Langmuir : the ACS journal of surfaces and colloids (2021): 10223-10232

Amplification-free detection of bacterial genes using a signaling probe-based DNA microarray.
Authors: Taguchi, Tomoyuki and Ishikawa, Machi and Ichikawa, Momoko and Tadenuma, Takashi and Hirakawa, Yuko and Yoshino, Tomoko and Maeda, Yoshiaki and Takeuchi, Hiyori and Nojima, Daisuke and Tanaami, Takeo and Matsunaga, Tadashi and Tanaka, Tsuyoshi
Journal: Biosensors & bioelectronics (2021): 113659

Species-specific differentiation of variola, monkeypox, and varicella-zoster viruses by multiplex real-time PCR assay.
Authors: Maksyutov, Rinat A and Gavrilova, Elena V and Shchelkunov, Sergei N
Journal: Journal of virological methods (2016): 215-220

Real-time stability testing of air-dried primers and fluorogenic hydrolysis probes stabilized by trehalose and xanthan.
Authors: Rombach, Markus and Kosse, Dominique and Faltin, Bernd and Wadle, Simon and Roth, Günter and Zengerle, Roland and von Stetten, Felix
Journal: BioTechniques (2014): 151-5

Species-specific identification of variola, monkeypox, cowpox, and vaccinia viruses by multiplex real-time PCR assay.
Authors: Shchelkunov, Sergei N and Shcherbakov, Dmitrii N and Maksyutov, Rinat A and Gavrilova, Elena V
Journal: Journal of virological methods (2011): 163-9

[TaqMan probes based on oligonucleotide-hairpin minor groove ligand conjugates].
Authors: Kostina, E V and Riabinin, V A and Maksakova, G A and Siniakov, A N
Journal: Bioorganicheskaia khimiia : 661-3

[Identification of human pathogenic variola and monkeypox viruses by real-time polymerase chain reaction].
Authors: Kostina, E V and Gavrilova, E V and Riabinin, V A and Shchelkunov, S N and Siniakov, A N
Journal: Voprosy virusologii : 28-33