AzoDye-2-PEG10 Azide
Ordering information
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 1015.13 |
Solvent | DMSO |
Spectral properties
Absorbance (nm) | 554 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
Alternative formats
AzoDye-2 PEG10 Alkyne |
Overview | ![]() ![]() |
See also: Förster Resonance Energy Transfer (FRET)
Molecular weight 1015.13 | Absorbance (nm) 554 |
AzoDye-2 PEG 10 azide is an excellent building block for labeling alkyne-containing molecules. It contains a PEG10 spacer to increase its water solubility. AzoDye-2 dyes are nearly non-fluorescent with moderate extinction coefficients. These dark quenchers are most typically used in Molecular Beacon probes in which FRET has minimal contribution to their fluorescence quenching, thus dependence on donor-acceptor spectral overlap is not significant as to other FRET probes such as FRET peptides. AzoDye-2 dyes can quench a variety of fluorophores, in particular, fluorescein derivatives such as FAM, HEX, TET, NED and JOE. This may reflect the AzoDye-2's ability to form stable, non-fluorescent complexes with a fluorophore.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of AzoDye-2-PEG10 Azide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 98.51 µL | 492.548 µL | 985.096 µL | 4.925 mL | 9.851 mL |
5 mM | 19.702 µL | 98.51 µL | 197.019 µL | 985.096 µL | 1.97 mL |
10 mM | 9.851 µL | 49.255 µL | 98.51 µL | 492.548 µL | 985.096 µL |
Molarity calculator
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Images
References
View all 9 references: Citation Explorer
FRET-based hACE2 receptor mimic peptide conjugated nanoprobe for simple detection of SARS-CoV-2.
Authors: Kang, Byunghoon and Lee, Youngjin and Lim, Jaewoo and Yong, Dongeun and Ki Choi, Young and Woo Yoon, Sun and Seo, Seungbeom and Jang, Soojin and Uk Son, Seong and Kang, Taejoon and Jung, Juyeon and Lee, Kyu-Sun and Kim, Myung Hee and Lim, Eun-Kyung
Journal: Chemical engineering journal (Lausanne, Switzerland : 1996) (2022): 136143
Authors: Kang, Byunghoon and Lee, Youngjin and Lim, Jaewoo and Yong, Dongeun and Ki Choi, Young and Woo Yoon, Sun and Seo, Seungbeom and Jang, Soojin and Uk Son, Seong and Kang, Taejoon and Jung, Juyeon and Lee, Kyu-Sun and Kim, Myung Hee and Lim, Eun-Kyung
Journal: Chemical engineering journal (Lausanne, Switzerland : 1996) (2022): 136143
Capability of novel fluorescence DNA-conjugated CdTe/ZnS quantum dots nanoprobe for COVID-19 sensing.
Authors: Bardajee, Ghasem Rezanejade and Zamani, Mohammadreza and Mahmoodian, Hossein and Elmizadeh, Hamideh and Yari, Hadi and Jouyandeh, Lavin and Shirkavand, Razieh and Sharifi, Mahdieh
Journal: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy (2022): 120702
Authors: Bardajee, Ghasem Rezanejade and Zamani, Mohammadreza and Mahmoodian, Hossein and Elmizadeh, Hamideh and Yari, Hadi and Jouyandeh, Lavin and Shirkavand, Razieh and Sharifi, Mahdieh
Journal: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy (2022): 120702
Efficient and Versatile Application of Fluorescence DNA-Conjugated CdTe Quantum Dots Nanoprobe for Detection of a Specific Target DNA of SARS Cov-2 Virus.
Authors: Bardajee, Ghasem Rezanejade and Zamani, Mohammadreza and Sharifi, Mahdieh
Journal: Langmuir : the ACS journal of surfaces and colloids (2021): 10223-10232
Authors: Bardajee, Ghasem Rezanejade and Zamani, Mohammadreza and Sharifi, Mahdieh
Journal: Langmuir : the ACS journal of surfaces and colloids (2021): 10223-10232
Amplification-free detection of bacterial genes using a signaling probe-based DNA microarray.
Authors: Taguchi, Tomoyuki and Ishikawa, Machi and Ichikawa, Momoko and Tadenuma, Takashi and Hirakawa, Yuko and Yoshino, Tomoko and Maeda, Yoshiaki and Takeuchi, Hiyori and Nojima, Daisuke and Tanaami, Takeo and Matsunaga, Tadashi and Tanaka, Tsuyoshi
Journal: Biosensors & bioelectronics (2021): 113659
Authors: Taguchi, Tomoyuki and Ishikawa, Machi and Ichikawa, Momoko and Tadenuma, Takashi and Hirakawa, Yuko and Yoshino, Tomoko and Maeda, Yoshiaki and Takeuchi, Hiyori and Nojima, Daisuke and Tanaami, Takeo and Matsunaga, Tadashi and Tanaka, Tsuyoshi
Journal: Biosensors & bioelectronics (2021): 113659
Species-specific differentiation of variola, monkeypox, and varicella-zoster viruses by multiplex real-time PCR assay.
Authors: Maksyutov, Rinat A and Gavrilova, Elena V and Shchelkunov, Sergei N
Journal: Journal of virological methods (2016): 215-220
Authors: Maksyutov, Rinat A and Gavrilova, Elena V and Shchelkunov, Sergei N
Journal: Journal of virological methods (2016): 215-220
Real-time stability testing of air-dried primers and fluorogenic hydrolysis probes stabilized by trehalose and xanthan.
Authors: Rombach, Markus and Kosse, Dominique and Faltin, Bernd and Wadle, Simon and Roth, Günter and Zengerle, Roland and von Stetten, Felix
Journal: BioTechniques (2014): 151-5
Authors: Rombach, Markus and Kosse, Dominique and Faltin, Bernd and Wadle, Simon and Roth, Günter and Zengerle, Roland and von Stetten, Felix
Journal: BioTechniques (2014): 151-5
Species-specific identification of variola, monkeypox, cowpox, and vaccinia viruses by multiplex real-time PCR assay.
Authors: Shchelkunov, Sergei N and Shcherbakov, Dmitrii N and Maksyutov, Rinat A and Gavrilova, Elena V
Journal: Journal of virological methods (2011): 163-9
Authors: Shchelkunov, Sergei N and Shcherbakov, Dmitrii N and Maksyutov, Rinat A and Gavrilova, Elena V
Journal: Journal of virological methods (2011): 163-9
[TaqMan probes based on oligonucleotide-hairpin minor groove ligand conjugates].
Authors: Kostina, E V and Riabinin, V A and Maksakova, G A and Siniakov, A N
Journal: Bioorganicheskaia khimiia : 661-3
Authors: Kostina, E V and Riabinin, V A and Maksakova, G A and Siniakov, A N
Journal: Bioorganicheskaia khimiia : 661-3
[Identification of human pathogenic variola and monkeypox viruses by real-time polymerase chain reaction].
Authors: Kostina, E V and Gavrilova, E V and Riabinin, V A and Shchelkunov, S N and Siniakov, A N
Journal: Voprosy virusologii : 28-33
Authors: Kostina, E V and Gavrilova, E V and Riabinin, V A and Shchelkunov, S N and Siniakov, A N
Journal: Voprosy virusologii : 28-33
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A New Protein Crosslinking Method for Labeling and Modifying Antibodies
A New Red Fluorescent & Robust Screen Quest™ Rhod-4™ Ca2+Indicator for Screening GPCR & Ca2+ Channel Targets
A New Robust No-Wash FLIPR Calcium Assay Kit for Screening GPCR and Calcium Channel Targets