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AzoDye-3 PEG10 Azide

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Physical properties
Molecular weight1155.28
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
Alternative formats
AzoDye-3 PEG10 Alkyne


Molecular weight
AzoDye-3 PEG 10 azide is an excellent building block for labeling alkyne-containing molecules. It contains a PEG10 spacer to increase its water solubility. AzoDye-3 dyes are nearly non-fluorescent with moderate extinction coefficients. These dark quenchers are most typically used in Molecular Beacon probes in which FRET has minimal contribution to their fluorescence quenching, thus dependence on donor-acceptor spectral overlap is not significant as to other FRET probes such as FRET peptides. AzoDye-3 dyes can quench a variety of fluorophores, in particular, Cy5 derivatives such as Cy5, Cy5.5, DyLight 647, Alexa Fluor® 647 and iFluor® 647. This may reflect the AzoDye-3's ability to form stable, non-fluorescent complexes with a fluorophore.


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of AzoDye-3 PEG10 Azide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM86.559 µL432.796 µL865.591 µL4.328 mL8.656 mL
5 mM17.312 µL86.559 µL173.118 µL865.591 µL1.731 mL
10 mM8.656 µL43.28 µL86.559 µL432.796 µL865.591 µL

Molarity calculator

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Spectral properties



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