AzoDye-3 PEG10 Azide
Ordering information
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 1155.28 |
Solvent | DMSO |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
Alternative formats
AzoDye-3 PEG10 Alkyne |
Overview | ![]() ![]() |
See also: Förster Resonance Energy Transfer (FRET)
Molecular weight 1155.28 |
AzoDye-3 PEG 10 azide is an excellent building block for labeling alkyne-containing molecules. It contains a PEG10 spacer to increase its water solubility. AzoDye-3 dyes are nearly non-fluorescent with moderate extinction coefficients. These dark quenchers are most typically used in Molecular Beacon probes in which FRET has minimal contribution to their fluorescence quenching, thus dependence on donor-acceptor spectral overlap is not significant as to other FRET probes such as FRET peptides. AzoDye-3 dyes can quench a variety of fluorophores, in particular, Cy5 derivatives such as Cy5, Cy5.5, DyLight 647, Alexa Fluor® 647 and iFluor® 647. This may reflect the AzoDye-3's ability to form stable, non-fluorescent complexes with a fluorophore.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of AzoDye-3 PEG10 Azide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 86.559 µL | 432.796 µL | 865.591 µL | 4.328 mL | 8.656 mL |
5 mM | 17.312 µL | 86.559 µL | 173.118 µL | 865.591 µL | 1.731 mL |
10 mM | 8.656 µL | 43.28 µL | 86.559 µL | 432.796 µL | 865.591 µL |
Molarity calculator
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Images
Figure 1. AzoDye-3 PEG 10 azide is an excellent building block for labeling alkyne-containing molecules. It contains a PEG10 spacer to increase its water solubility. AzoDye-3 dyes are nearly non-fluorescent with moderate extinction coefficients. AzoDye-3 dyes can quench a variety of fluorophores, in particular, Cy5 derivatives such as Cy5, Cy5.5, DyLight 647, Alexa Fluor® 647 and iFluor® 647.
References
View all 15 references: Citation Explorer
Multivalent self-assembled nano string lights for tumor-targeted delivery and accelerated biomarker imaging in living cells and in vivo.
Authors: Li, Zhijun and Li, Qiannan and Wu, Yanan and Yuan, Kun and Shi, Mingqing and Li, Yiwei and Meng, Hong-Min and Li, Zhaohui
Journal: The Analyst (2022): 811-818
Authors: Li, Zhijun and Li, Qiannan and Wu, Yanan and Yuan, Kun and Shi, Mingqing and Li, Yiwei and Meng, Hong-Min and Li, Zhaohui
Journal: The Analyst (2022): 811-818
Broad-Spectrum Polymeric Nanoquencher as an Efficient Fluorescence Sensing Platform for Biomolecular Detection.
Authors: Dong, Xiao and Ong, Sing Yee and Zhang, Changyu and Chen, Wenqiang and Du, Shubo and Xiao, Qicai and Gao, Liqian and Yao, Shao Q
Journal: ACS sensors (2021): 3102-3111
Authors: Dong, Xiao and Ong, Sing Yee and Zhang, Changyu and Chen, Wenqiang and Du, Shubo and Xiao, Qicai and Gao, Liqian and Yao, Shao Q
Journal: ACS sensors (2021): 3102-3111
Lighting up ATP in cells and tissues using a simple aptamer-based fluorescent probe.
Authors: Liu, Wenjun and Zhu, Xuena and Mozneb, Maedeh and Nagahara, Larry and Hu, Tony Y and Li, Chen-Zhong
Journal: Mikrochimica acta (2021): 352
Authors: Liu, Wenjun and Zhu, Xuena and Mozneb, Maedeh and Nagahara, Larry and Hu, Tony Y and Li, Chen-Zhong
Journal: Mikrochimica acta (2021): 352
A Dendron-Based Fluorescence Turn-On Probe for Tumor Detection.
Authors: Liu, Changren and Zhang, Ling'e and Zhou, Sensen and Zhang, Xiaoke and Wu, Wei and Jiang, Xiqun
Journal: Chemistry (Weinheim an der Bergstrasse, Germany) (2020): 13022-13030
Authors: Liu, Changren and Zhang, Ling'e and Zhou, Sensen and Zhang, Xiaoke and Wu, Wei and Jiang, Xiqun
Journal: Chemistry (Weinheim an der Bergstrasse, Germany) (2020): 13022-13030
Aptamer-Functionalized Activatable DNA Tetrahedron Nanoprobe for PIWI-Interacting RNA Imaging and Regulating in Cancer Cells.
Authors: Jia, Ruichen and He, Xiaoxiao and Ma, Wenjie and Lei, Yanli and Cheng, Hong and Sun, Huanhuan and Huang, Jin and Wang, Kemin
Journal: Analytical chemistry (2019): 15107-15113
Authors: Jia, Ruichen and He, Xiaoxiao and Ma, Wenjie and Lei, Yanli and Cheng, Hong and Sun, Huanhuan and Huang, Jin and Wang, Kemin
Journal: Analytical chemistry (2019): 15107-15113
Optical Aptamer Probes of Fluorescent Imaging to Rapid Monitoring of Circulating Tumor Cell.
Authors: Hwang, Ji Yeon and Kim, Sang Tae and Han, Ho-Seong and Kim, Kyunggon and Han, Jin Soo
Journal: Sensors (Basel, Switzerland) (2016)
Authors: Hwang, Ji Yeon and Kim, Sang Tae and Han, Ho-Seong and Kim, Kyunggon and Han, Jin Soo
Journal: Sensors (Basel, Switzerland) (2016)
A color-tunable molecular beacon to sense miRNA-9 expression during neurogenesis.
Authors: Ko, Hae Young and Lee, Jonghwan and Joo, Jin Young and Lee, Yong Seung and Heo, Hyejung and Ko, Jung Jae and Kim, Soonhag
Journal: Scientific reports (2014): 4626
Authors: Ko, Hae Young and Lee, Jonghwan and Joo, Jin Young and Lee, Yong Seung and Heo, Hyejung and Ko, Jung Jae and Kim, Soonhag
Journal: Scientific reports (2014): 4626
Optimal dye-quencher pairs for the design of an "activatable" nanoprobe for optical imaging.
Authors: Simard, Bryan and Tomanek, Boguslaw and van Veggel, Frank C J M and Abulrob, Abedelnasser
Journal: Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society (2013): 1824-9
Authors: Simard, Bryan and Tomanek, Boguslaw and van Veggel, Frank C J M and Abulrob, Abedelnasser
Journal: Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society (2013): 1824-9
Optical aptasensors for the analysis of the vascular endothelial growth factor (VEGF).
Authors: Freeman, Ronit and Girsh, Julia and Jou, Amily Fang-ju and Ho, Ja-an Annie and Hug, Thomas and Dernedde, Jens and Willner, Itamar
Journal: Analytical chemistry (2012): 6192-8
Authors: Freeman, Ronit and Girsh, Julia and Jou, Amily Fang-ju and Ho, Ja-an Annie and Hug, Thomas and Dernedde, Jens and Willner, Itamar
Journal: Analytical chemistry (2012): 6192-8
Nonfluorescent quenchers to correlate single-molecule conformational and compositional dynamics.
Authors: Chen, Jin and Tsai, Albert and Petrov, Alexey and Puglisi, Joseph D
Journal: Journal of the American Chemical Society (2012): 5734-7
Authors: Chen, Jin and Tsai, Albert and Petrov, Alexey and Puglisi, Joseph D
Journal: Journal of the American Chemical Society (2012): 5734-7
Application notes
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A New Robust No-Wash FLIPR Calcium Assay Kit for Screening GPCR and Calcium Channel Targets