logo
AAT Bioquest

AzoDye-3 PEG10 Azide

Product Image
Product Image
Gallery Image 1
Ordering information
Price
Catalog Number
Unit Size
Quantity
Add to cart
Additional ordering information
Telephone1-800-990-8053
Fax1-800-609-2943
Emailsales@aatbio.com
InternationalSee distributors
Bulk requestInquire
Custom sizeInquire
ShippingStandard overnight for United States, inquire for international
Request quotation
Physical properties
Molecular weight1169.31
SolventDMSO
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
Alternative formats
AzoDye-3 PEG10 Alkyne

OverviewpdfSDSpdfProtocol


Molecular weight
1169.31
AzoDye-3 PEG 10 azide is an excellent building block for labeling alkyne-containing molecules. It contains a PEG10 spacer to increase its water solubility. AzoDye-3 dyes are nearly non-fluorescent with moderate extinction coefficients. These dark quenchers are most typically used in Molecular Beacon probes in which FRET has minimal contribution to their fluorescence quenching, thus dependence on donor-acceptor spectral overlap is not significant as to other FRET probes such as FRET peptides. AzoDye-3 dyes can quench a variety of fluorophores, in particular, Cy5 derivatives such as Cy5, Cy5.5, DyLight 647, Alexa Fluor® 647 and iFluor® 647. This may reflect the AzoDye-3's ability to form stable, non-fluorescent complexes with a fluorophore.

Calculators


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of AzoDye-3 PEG10 Azide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM85.521 µL427.603 µL855.205 µL4.276 mL8.552 mL
5 mM17.104 µL85.521 µL171.041 µL855.205 µL1.71 mL
10 mM8.552 µL42.76 µL85.521 µL427.603 µL855.205 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles
/=x=

Spectrum


Open in Advanced Spectrum Viewer
spectrum

Spectral properties

Images


References


View all 15 references: Citation Explorer
Multivalent self-assembled nano string lights for tumor-targeted delivery and accelerated biomarker imaging in living cells and in vivo.
Authors: Li, Zhijun and Li, Qiannan and Wu, Yanan and Yuan, Kun and Shi, Mingqing and Li, Yiwei and Meng, Hong-Min and Li, Zhaohui
Journal: The Analyst (2022): 811-818
Broad-Spectrum Polymeric Nanoquencher as an Efficient Fluorescence Sensing Platform for Biomolecular Detection.
Authors: Dong, Xiao and Ong, Sing Yee and Zhang, Changyu and Chen, Wenqiang and Du, Shubo and Xiao, Qicai and Gao, Liqian and Yao, Shao Q
Journal: ACS sensors (2021): 3102-3111
Lighting up ATP in cells and tissues using a simple aptamer-based fluorescent probe.
Authors: Liu, Wenjun and Zhu, Xuena and Mozneb, Maedeh and Nagahara, Larry and Hu, Tony Y and Li, Chen-Zhong
Journal: Mikrochimica acta (2021): 352
A Dendron-Based Fluorescence Turn-On Probe for Tumor Detection.
Authors: Liu, Changren and Zhang, Ling'e and Zhou, Sensen and Zhang, Xiaoke and Wu, Wei and Jiang, Xiqun
Journal: Chemistry (Weinheim an der Bergstrasse, Germany) (2020): 13022-13030
Aptamer-Functionalized Activatable DNA Tetrahedron Nanoprobe for PIWI-Interacting RNA Imaging and Regulating in Cancer Cells.
Authors: Jia, Ruichen and He, Xiaoxiao and Ma, Wenjie and Lei, Yanli and Cheng, Hong and Sun, Huanhuan and Huang, Jin and Wang, Kemin
Journal: Analytical chemistry (2019): 15107-15113
Optical Aptamer Probes of Fluorescent Imaging to Rapid Monitoring of Circulating Tumor Cell.
Authors: Hwang, Ji Yeon and Kim, Sang Tae and Han, Ho-Seong and Kim, Kyunggon and Han, Jin Soo
Journal: Sensors (Basel, Switzerland) (2016)
A color-tunable molecular beacon to sense miRNA-9 expression during neurogenesis.
Authors: Ko, Hae Young and Lee, Jonghwan and Joo, Jin Young and Lee, Yong Seung and Heo, Hyejung and Ko, Jung Jae and Kim, Soonhag
Journal: Scientific reports (2014): 4626
Optimal dye-quencher pairs for the design of an "activatable" nanoprobe for optical imaging.
Authors: Simard, Bryan and Tomanek, Boguslaw and van Veggel, Frank C J M and Abulrob, Abedelnasser
Journal: Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society (2013): 1824-9
Optical aptasensors for the analysis of the vascular endothelial growth factor (VEGF).
Authors: Freeman, Ronit and Girsh, Julia and Jou, Amily Fang-ju and Ho, Ja-an Annie and Hug, Thomas and Dernedde, Jens and Willner, Itamar
Journal: Analytical chemistry (2012): 6192-8
Nonfluorescent quenchers to correlate single-molecule conformational and compositional dynamics.
Authors: Chen, Jin and Tsai, Albert and Petrov, Alexey and Puglisi, Joseph D
Journal: Journal of the American Chemical Society (2012): 5734-7