AzoDye-3 PEG10 Azide

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Telephone	1-800-990-8053
Fax	1-800-609-2943
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Physical properties

Molecular weight	1155.28
Solvent	DMSO

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure

Alternative formats

AzoDye-3 PEG10 Alkyne

Overview SDS Protocol

Molecular weight

1155.28

AzoDye-3 PEG 10 azide is an excellent building block for labeling alkyne-containing molecules. It contains a PEG10 spacer to increase its water solubility. AzoDye-3 dyes are nearly non-fluorescent with moderate extinction coefficients. These dark quenchers are most typically used in Molecular Beacon probes in which FRET has minimal contribution to their fluorescence quenching, thus dependence on donor-acceptor spectral overlap is not significant as to other FRET probes such as FRET peptides. AzoDye-3 dyes can quench a variety of fluorophores, in particular, Cy5 derivatives such as Cy5, Cy5.5, DyLight 647, Alexa Fluor® 647 and iFluor® 647. This may reflect the AzoDye-3's ability to form stable, non-fluorescent complexes with a fluorophore.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of AzoDye-3 PEG10 Azide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	86.559 µL	432.796 µL	865.591 µL	4.328 mL	8.656 mL
5 mM	17.312 µL	86.559 µL	173.118 µL	865.591 µL	1.731 mL
10 mM	8.656 µL	43.28 µL	86.559 µL	432.796 µL	865.591 µL

Molarity calculator

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Spectrum

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Spectral properties

Images

Figure 1. AzoDye-3 PEG 10 azide is an excellent building block for labeling alkyne-containing molecules. It contains a PEG10 spacer to increase its water solubility. AzoDye-3 dyes are nearly non-fluorescent with moderate extinction coefficients. AzoDye-3 dyes can quench a variety of fluorophores, in particular, Cy5 derivatives such as Cy5, Cy5.5, DyLight 647, Alexa Fluor® 647 and iFluor® 647.

References

View all 15 references: Citation Explorer

Multivalent self-assembled nano string lights for tumor-targeted delivery and accelerated biomarker imaging in living cells and in vivo.
Authors: Li, Zhijun and Li, Qiannan and Wu, Yanan and Yuan, Kun and Shi, Mingqing and Li, Yiwei and Meng, Hong-Min and Li, Zhaohui
Journal: The Analyst (2022): 811-818

Broad-Spectrum Polymeric Nanoquencher as an Efficient Fluorescence Sensing Platform for Biomolecular Detection.
Authors: Dong, Xiao and Ong, Sing Yee and Zhang, Changyu and Chen, Wenqiang and Du, Shubo and Xiao, Qicai and Gao, Liqian and Yao, Shao Q
Journal: ACS sensors (2021): 3102-3111

Lighting up ATP in cells and tissues using a simple aptamer-based fluorescent probe.
Authors: Liu, Wenjun and Zhu, Xuena and Mozneb, Maedeh and Nagahara, Larry and Hu, Tony Y and Li, Chen-Zhong
Journal: Mikrochimica acta (2021): 352

A Dendron-Based Fluorescence Turn-On Probe for Tumor Detection.
Authors: Liu, Changren and Zhang, Ling'e and Zhou, Sensen and Zhang, Xiaoke and Wu, Wei and Jiang, Xiqun
Journal: Chemistry (Weinheim an der Bergstrasse, Germany) (2020): 13022-13030

Aptamer-Functionalized Activatable DNA Tetrahedron Nanoprobe for PIWI-Interacting RNA Imaging and Regulating in Cancer Cells.
Authors: Jia, Ruichen and He, Xiaoxiao and Ma, Wenjie and Lei, Yanli and Cheng, Hong and Sun, Huanhuan and Huang, Jin and Wang, Kemin
Journal: Analytical chemistry (2019): 15107-15113

Optical Aptamer Probes of Fluorescent Imaging to Rapid Monitoring of Circulating Tumor Cell.
Authors: Hwang, Ji Yeon and Kim, Sang Tae and Han, Ho-Seong and Kim, Kyunggon and Han, Jin Soo
Journal: Sensors (Basel, Switzerland) (2016)

A color-tunable molecular beacon to sense miRNA-9 expression during neurogenesis.
Authors: Ko, Hae Young and Lee, Jonghwan and Joo, Jin Young and Lee, Yong Seung and Heo, Hyejung and Ko, Jung Jae and Kim, Soonhag
Journal: Scientific reports (2014): 4626

Optimal dye-quencher pairs for the design of an "activatable" nanoprobe for optical imaging.
Authors: Simard, Bryan and Tomanek, Boguslaw and van Veggel, Frank C J M and Abulrob, Abedelnasser
Journal: Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society (2013): 1824-9

Optical aptasensors for the analysis of the vascular endothelial growth factor (VEGF).
Authors: Freeman, Ronit and Girsh, Julia and Jou, Amily Fang-ju and Ho, Ja-an Annie and Hug, Thomas and Dernedde, Jens and Willner, Itamar
Journal: Analytical chemistry (2012): 6192-8

Nonfluorescent quenchers to correlate single-molecule conformational and compositional dynamics.
Authors: Chen, Jin and Tsai, Albert and Petrov, Alexey and Puglisi, Joseph D
Journal: Journal of the American Chemical Society (2012): 5734-7