Name: Beadlite™ Rapid Colorimetric Maleimide Quantitation Kit for Nanoparticles
Brand: AAT Bioquest
SKU: 5533
Price: 308 USD
Availability: InStock

Beadlite™ Rapid Colorimetric Maleimide Quantitation Kit for Nanoparticles

The Beadlite™ Rapid Colorimetric Maleimide Quantitation Kit is an essential tool for researchers involved in bioconjugation research, specifically designed for the accurate quantification of maleimide functional groups on solid surfaces such as magnetic beads, polystyrene beads, or other microsphere nanoparticles. The kit employs the principle of Ellman's Reagent (5,5'-Dithio-bis-(2-nitrobenzoic acid) or DTNB) for the quantitative determination of free sulfhydryl groups, a method widely recognized for its sensitivity and reliability. In the assay procedure, a sample is initially reacted with an excess of mercaptoethylamine (MEA), followed by the quantification of the remaining unreacted MEA using DTNB. The formation of a highly colored 5-thio-2-nitrobenzoic acid (TNB) anion, measurable spectrophotometrically at an absorbance maximum of 412 nm (Ɛ = 14,150 M^-1cm^-1), allows for the precise determination of maleimide functional groups present on the surface of the microspheres and nanoparticles. The Beadlite™ Rapid Colorimetric Maleimide Quantitation Kit's rapid and straightforward procedure makes it suitable for a wide range of applications in bioconjugation research, including the quantification of maleimide functional groups on microspheres and nanoparticles, characterization of maleimide-modified surfaces, and evaluation of maleimide-based crosslinking and conjugation reactions. Each kit provides sufficient reagents for 100 assays, with a volume of 0.5 mL per assay.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
5533	100 Tests	Price

References

View all 50 references: Citation Explorer

Chemically-Modified Sepharose 6B Beads for Collection of Circulating Tumor Cells.

Authors:

Chen, Haiyan and Zhang, Yiming and Ma, Xiaoxiao and Zhou, Bohao and Liu, Zhonghua

Journal:

Biomolecules (2023)

Biotransformation of Trastuzumab and Pertuzumab in Breast Cancer Patients Assessed by Affinity Enrichment and Ion-Exchange Chromatography.

Authors:

Olaleye, Oladapo and Spanov, Baubek and Bults, Peter and van der Voort, Anna and Govorukhina, Natalia and Sonke, Gabe S and Horvatovich, Peter and van de Merbel, Nico C and Bischoff, Rainer

Journal:

Drug metabolism and disposition: the biological fate of chemicals (2023): 249-256

Electrochemical biosensor for the dual detection of Gambierdiscus australes and Gambierdiscus excentricus in field samples. First report of G. excentricus in the Balearic Islands.

Authors:

Gaiani, Greta and Cucchi, Francesca and Toldrà, Anna and Andree, Karl B and Rey, María and Tsumuraya, Takeshi and O'Sullivan, Ciara K and Diogène, Jorge and Campàs, Mònica

Journal:

The Science of the total environment (2022): 150915

Facilitating EMA binding test performance using fluorescent beads combined with next-generation sequencing.

Authors:

Glenthøj, Andreas and Brieghel, Christian and Nardo-Marino, Amina and van Wijk, Richard and Birgens, Henrik and Petersen, Jesper

Journal:

EJHaem (2021): 716-728

Enrichment and Liquid Chromatography-Mass Spectrometry Analysis of Trastuzumab and Pertuzumab Using Affimer Reagents.

Authors:

Olaleye, Oladapo and Spanov, Baubek and Ford, Robert and Govorukhina, Natalia and van de Merbel, Nico C and Bischoff, Rainer

Journal:

Analytical chemistry (2021): 13597-13605

Usage and storage

Intended use

Research Use Only (RUO)

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Page updated on July 14, 2026

Component A: MEA	1 Vial
Component B: DTNB	1 Vial
Component C: Assay Buffer	1 Bottle (50 mL)
Component D: DMSO	1 Vial (1 mL)