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Beadlite™ Rapid Colorimetric Maleimide Quantitation Kit for Nanoparticles
Optimized for 680nm Absorption
Maleimide-thiol chemistry is integral to the engineering of antibody-nanoparticle (NP) conjugates, with nanoparticle surface activation typically achieved via SMCC-mediated maleimide functionalization. A major challenge in maleimide crosslinking technology is the accurate quantification of maleimide groups covalently attached to solid surfaces. The Beadlite™ Rapid Colorimetric Maleimide Quantitation Kit for Nanoparticles Optimized for 680nm Absorption effectively addresses this issue by enabling quick and precise measurements of maleimide functional groups on solid surfaces such as magnetic beads, polystyrene beads, and other nanoparticle types. The kit features Maleimide 680™, which absorbs maximally at 680 nm and is used for the quantitative determination of free sulfhydryl groups. The assay is based on the principle of reacting nanoparticles with an excess of Maleimide 680™, followed by the quantification of unreacted Maleimide 680™ using its known molar extinction coefficient of 250,000 M-1cm-1. The extent of maleimide functionalization on the NP surface is determined by calculating the difference between the initially added and the unreacted Maleimide 680™, allowing for an accurate assessment of maleimide group presence post-reaction. This kit's rapid and straightforward procedure is particularly suitable for applications in bioconjugation research, including the quantification of maleimide functional groups on microspheres and nanoparticles, characterization of maleimide-modified surfaces, and evaluation of maleimide-based crosslinking and conjugation reactions.
General packaging for Cat. No. 5534. Your actual packaging may differ.
General packaging for Cat. No. 5534. Your actual packaging may differ.
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
5534100 Tests
Price
 
Physical properties
SolventDMSO
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501
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Page updated on May 5, 2026