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Biotin-4-fluorescein
CAS 1032732-74-3
This bifunctional biotin-fluorescein conjugate demonstrates better binding and stronger fluorescence than biotin fluorescein. It has similar avidin-binding properties in terms of high affinity, fast association, and non-cooperative binding to avidin and streptavidin tetramers. These exceptional properties are attributed to the small size/length of the new ligand since all larger/longer biotin derivatives are known for their mutual steric hindrance and anti-cooperative binding in 4:1 complexes with avidin and streptavidin tetramers. Specific binding of this biotin-fluorescein conjugate towards avidin and streptavidin is accompanied by 84-88% quenching of ligand fluorescence. It is used for the quantitation of biotin-binding sites. Both the fluorescence and absorbance of biotin-4-fluorescein are quenched upon binding to one of the four biotin-binding sites of streptavidin, avidin, or the streptavidin conjugates of fluorescent dyes or enzymes. As a result, when a known concentration of biotin-4-fluorescein is added to a known amount of streptavidin, one can estimate the number of biotin-binding sites.
Chemical structure for Biotin-4-fluorescein *CAS 1032732-74-3*
Chemical structure for Biotin-4-fluorescein *CAS 1032732-74-3*
CatalogSize
Price
Quantity
30065 mg
Price
 
Physical properties

Molecular weight644.69
SolventDMSO
Spectral properties

Absorbance (nm)487
Correction factor (260 nm)0.32
Correction factor (280 nm)0.35
Extinction coefficient (cm -1 M -1)
80000
1
Excitation (nm)498
Emission (nm)517
Quantum yield
0.7900
1
0.95
2
Storage, safety and handling

Certificate of OriginDownload PDF
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure; Desiccated
UNSPSC12352200
CAS1032732-74-3
Instrument settings

Fluorescence microplate reader
Excitation492 nm
Emission518 nm
Cutoff500 nm
Recommended plateSolid black
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Page updated on October 13, 2025