BOC-Val-Pro-Arg-AMC
Ordering information
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 741.75 |
Solvent | DMSO |
Spectral properties
Excitation (nm) | 341 |
Emission (nm) | 441 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12352200 |
Overview | SDSProtocol |
Molecular weight 741.75 | Excitation (nm) 341 | Emission (nm) 441 |
BOC-Val-Pro-Arg-AMC is a sensitive fluorogenic substrate for alpha-thrombin. The non-fluorescent substrate generates a bright blue fluorescent AMC product that has an emission spectra that can be easily detected with the DAPI filter set. The Km values of alpha-thrombin and the alpha-thrombin-staphylocoagulase complex for BOC-Val-Pro-Arg-AMC were reported to be 21 and 25 uM respectively. The Kcat values of alpha-thrombin and the alpha-thrombin-staphylocoagulase complex for BOC-Val-Pro-Arg-AMC were reported to be 105 and 89 s-1 respectively. This substrate has also been used for the rapid detection of MRSA (methicillin-resistant Staphylococcus aureus).
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of BOC-Val-Pro-Arg-AMC to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 134.816 µL | 674.082 µL | 1.348 mL | 6.741 mL | 13.482 mL |
5 mM | 26.963 µL | 134.816 µL | 269.633 µL | 1.348 mL | 2.696 mL |
10 mM | 13.482 µL | 67.408 µL | 134.816 µL | 674.082 µL | 1.348 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Product Family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) |
BOC-Val-Pro-Arg-Aminoluciferin | 362 | 499 | 18000 |
Citations
View all 1 citations: Citation Explorer
Using tungsten oxide quantum-dot enhanced electrochemiluminescence to measure thrombin activity and screen its inhibitors
Authors: Zhang, Jing and Xu, Dan and Deng, Zijie and Tan, Xueping and Guo, Dongnan and Qiao, Yanru and Li, You and Hou, Xiaofang and Wang, Sicen and Zhang, Junbo
Journal: Talanta (2023): 125267
Authors: Zhang, Jing and Xu, Dan and Deng, Zijie and Tan, Xueping and Guo, Dongnan and Qiao, Yanru and Li, You and Hou, Xiaofang and Wang, Sicen and Zhang, Junbo
Journal: Talanta (2023): 125267
References
View all 120 references: Citation Explorer
A fluorescence quenching assay to discriminate between specific and nonspecific inhibitors of dengue virus protease
Authors: Bodenreider C, Beer D, Keller TH, Sonntag S, Wen D, Yap L, Yau YH, Shochat SG, Huang D, Zhou T, Caflisch A, Su XC, Ozawa K, Otting G, Vasudevan SG, Lescar J, Lim SP.
Journal: Anal Biochem (2009): 195
Authors: Bodenreider C, Beer D, Keller TH, Sonntag S, Wen D, Yap L, Yau YH, Shochat SG, Huang D, Zhou T, Caflisch A, Su XC, Ozawa K, Otting G, Vasudevan SG, Lescar J, Lim SP.
Journal: Anal Biochem (2009): 195
Prokaryotic expression and Identification of dual-fluorescence fusion proteins of small ubiquitin-like modifier and sentrin-specific protease
Authors: Wang X, Guo J, Wang J, Li S, Sun L, Lu J.
Journal: Sheng Wu Gong Cheng Xue Bao (2009): 701
Authors: Wang X, Guo J, Wang J, Li S, Sun L, Lu J.
Journal: Sheng Wu Gong Cheng Xue Bao (2009): 701
Real-time monitoring of human enterovirus (HEV)-infected cells and anti-HEV 3C protease potency by fluorescence resonance energy transfer
Authors: Tsai MT, Cheng YH, Liu YN, Liao NC, Lu WW, Kung SH.
Journal: Antimicrob Agents Chemother (2009): 748
Authors: Tsai MT, Cheng YH, Liu YN, Liao NC, Lu WW, Kung SH.
Journal: Antimicrob Agents Chemother (2009): 748
Thrombin-sensitive photodynamic agents: a novel strategy for selective synovectomy in rheumatoid arthritis
Authors: Gabriel D, Busso N, So A, van den Bergh H, Gurny R, Lange N.
Journal: J Control Release (2009): 225
Authors: Gabriel D, Busso N, So A, van den Bergh H, Gurny R, Lange N.
Journal: J Control Release (2009): 225
Direct comparison of fluorescence- and bioluminescence-based resonance energy transfer methods for real-time monitoring of thrombin-catalysed proteolytic cleavage
Authors: Dacres H, Dumancic MM, Horne I, Trowell SC.
Journal: Biosens Bioelectron (2009): 1164
Authors: Dacres H, Dumancic MM, Horne I, Trowell SC.
Journal: Biosens Bioelectron (2009): 1164
Dual-function probe to detect protease activity for fluorescence measurement and 19F MRI
Authors: Mizukami S, Takikawa R, Sugihara F, Shirakawa M, Kikuchi K.
Journal: Angew Chem Int Ed Engl (2009): 3641
Authors: Mizukami S, Takikawa R, Sugihara F, Shirakawa M, Kikuchi K.
Journal: Angew Chem Int Ed Engl (2009): 3641
Real-time detection of caspase-3-like protease activation in vivo using fluorescence resonance energy transfer during plant programmed cell death induced by ultraviolet C overexposure
Authors: Zhang L, Xu Q, Xing D, Gao C, Xiong H.
Journal: Plant Physiol (2009): 1773
Authors: Zhang L, Xu Q, Xing D, Gao C, Xiong H.
Journal: Plant Physiol (2009): 1773
Fluorescence resonance energy transfer-based assay for characterization of hepatitis C virus NS3-4A protease activity in live cells
Authors: Sabariegos R, Picazo F, Domingo B, Franco S, Martinez MA, Llopis J.
Journal: Antimicrob Agents Chemother (2009): 728
Authors: Sabariegos R, Picazo F, Domingo B, Franco S, Martinez MA, Llopis J.
Journal: Antimicrob Agents Chemother (2009): 728
Label-free, homogeneous, and fluorescence "turn-on" detection of protease using conjugated polyelectrolytes
Authors: An L, Liu L, Wang S.
Journal: Biomacromolecules (2009): 454
Authors: An L, Liu L, Wang S.
Journal: Biomacromolecules (2009): 454
A protease inhibitor discovery method using fluorescence correlation spectroscopy with position-specific labeled protein substrates
Authors: Nakata H, Ohtsuki T, Sisido M.
Journal: Anal Biochem (2009): 121
Authors: Nakata H, Ohtsuki T, Sisido M.
Journal: Anal Biochem (2009): 121
Application notes
Abbreviation of Common Chemical Compounds Related to Peptides
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A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
Buccutite™ Bioconjugation Technology
A New Protein Crosslinking Method for Labeling and Modifying Antibodies
A New Robust No-Wash FLIPR Calcium Assay Kit for Screening GPCR and Calcium Channel Targets
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
Buccutite™ Bioconjugation Technology