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BOC-Val-Pro-Arg-AMC

BOC-Val-Pro-Arg-AMC is a sensitive fluorogenic substrate for alpha-thrombin. The non-fluorescent substrate generates a bright blue fluorescent AMC product that has an emission spectra that can be easily detected with the DAPI filter set. The Km values of alpha-thrombin and the alpha-thrombin-staphylocoagulase complex for BOC-Val-Pro-Arg-AMC were reported to be 21 and 25 uM respectively. The Kcat values of alpha-thrombin and the alpha-thrombin-staphylocoagulase complex for BOC-Val-Pro-Arg-AMC were reported to be 105 and 89 s-1 respectively. This substrate has also been used for the rapid detection of MRSA (methicillin-resistant Staphylococcus aureus).

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of BOC-Val-Pro-Arg-AMC to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM134.816 µL674.082 µL1.348 mL6.741 mL13.482 mL
5 mM26.963 µL134.816 µL269.633 µL1.348 mL2.696 mL
10 mM13.482 µL67.408 µL134.816 µL674.082 µL1.348 mL

Molarity calculator

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Spectrum

Product family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)
BOC-Val-Pro-Arg-Aminoluciferin36249918000

Citations

View all 1 citations: Citation Explorer
Using tungsten oxide quantum-dot enhanced electrochemiluminescence to measure thrombin activity and screen its inhibitors
Authors: Zhang, Jing and Xu, Dan and Deng, Zijie and Tan, Xueping and Guo, Dongnan and Qiao, Yanru and Li, You and Hou, Xiaofang and Wang, Sicen and Zhang, Junbo
Journal: Talanta (2023): 125267

References

View all 120 references: Citation Explorer
Improved detection of ovarian cancer metastases by intraoperative quantitative fluorescence protease imaging in a pre-clinical model
Authors: Sheth RA, Upadhyay R, Stangenberg L, Sheth R, Weissleder R, Mahmood U.
Journal: Gynecol Oncol (2009): 616
A fluorescence lifetime-based assay for protease inhibitor profiling on human kallikrein 7
Authors: Doering K, Meder G, Hinnenberger M, Woelcke J, Mayr LM, Hassiepen U.
Journal: J Biomol Screen (2009): 1
A protease inhibitor discovery method using fluorescence correlation spectroscopy with position-specific labeled protein substrates
Authors: Nakata H, Ohtsuki T, Sisido M.
Journal: Anal Biochem (2009): 121
Label-free, homogeneous, and fluorescence "turn-on" detection of protease using conjugated polyelectrolytes
Authors: An L, Liu L, Wang S.
Journal: Biomacromolecules (2009): 454
Fluorescence resonance energy transfer-based assay for characterization of hepatitis C virus NS3-4A protease activity in live cells
Authors: Sabariegos R, Picazo F, Domingo B, Franco S, Martinez MA, Llopis J.
Journal: Antimicrob Agents Chemother (2009): 728
Page updated on October 11, 2024

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Catalog Number13461
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Physical properties

Molecular weight

741.75

Solvent

DMSO

Spectral properties

Excitation (nm)

341

Emission (nm)

441

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12352200
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