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Bromocresol purple *CAS 115-40-2* *Ultrapure grade*

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Physical properties
Molecular weight540.22
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageRoom temperature (10-25 °C); Minimize light exposure


Molecular weight
Bromocresol purple (BCP) or 5',5-dibromo-o-cresolsulfophthalein, is a pH indicator. In its sultone form, it has a pKa value of 6.3, and is usually prepared as a 0.04% aqueous solution. Besides its primary function as an indicator, bromocresol purple is used in medical laboratories to measure albumin.


Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of Bromocresol purple *CAS 115-40-2* *Ultrapure grade* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM185.11 µL925.549 µL1.851 mL9.255 mL18.511 mL
5 mM37.022 µL185.11 µL370.22 µL1.851 mL3.702 mL
10 mM18.511 µL92.555 µL185.11 µL925.549 µL1.851 mL

Molarity calculator

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View all 32 references: Citation Explorer
Albumin concentration determined by the modified bromocresol purple method is superior to that by the bromocresol green method for assessing nutritional status in malnourished patients with inflammation
Authors: Ueno T, Hirayama S, Ito M, Nishioka E, Fukushima Y, Satoh T, Idei M, Horiuchi Y, Shoji H, Ohmura H, Shimizu T, Miida T.
Journal: Ann Clin Biochem (2013): 576
Using polyvinyl chloride dyed with bromocresol purple in radiation dosimetry
Authors: Kattan M, al Kassiri H, Daher Y.
Journal: Appl Radiat Isot (2011): 377
Serum globulins contribute to the discrepancies observed between the bromocresol green and bromocresol purple assays of serum albumin concentration
Authors: Xu Y, Wang L, Wang J, Liang H, Jiang X.
Journal: Br J Biomed Sci (2011): 120
Mechanism for the color change in bromocresol purple bound to human serum albumin
Authors: Ito S, Yamamoto D.
Journal: Clin Chim Acta (2010): 294
Identification of two bromocresol purple binding sites on human serum albumin
Authors: Ito S, Yamamoto D.
Journal: Clin Chim Acta (2010): 1536
Spectroscopic studies on the binding of bromocresol purple to different serum albumins and its bilirubin displacing action
Authors: Faizul FM, Abdul Kadir H, Tayyab S.
Journal: J Photochem Photobiol B (2008): 1
Extractive spectrophotometric methods for the determination of oxomemazine hydrochloride in bulk and pharmaceutical formulations using bromocresol green, bromocresol purple and bromophenol blue
Authors: El-Didamony AM., undefined
Journal: Arch Pharm (Weinheim) (2005): 190
Spectrophotometric determination of indinavir in bulk and pharmaceutical formulations using bromocresol purple and bromothymol blue
Authors: Erk N., undefined
Journal: Pharmazie (2004): 183
Measurement of serum albumin by capillary zone electrophoresis, bromocresol green, bromocresol purple, and immunoassay methods
Authors: Duly EB, Grimason S, Grimason P, Barnes G, Trinick TR.
Journal: J Clin Pathol (2003): 780
Spectrophotometric determination of enoxacin as ion-pairs with bromophenol blue and bromocresol purple in bulk and pharmaceutical dosage form
Authors: Suslu I, Tamer A.
Journal: J Pharm Biomed Anal (2002): 545