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Bromocresol purple *CAS 115-40-2* *Ultrapure grade*
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Application notes
Bromocresol purple (BCP) or 5',5-dibromo-o-cresolsulfophthalein, is a pH indicator. In its sultone form, it has a pKa value of 6.3, and is usually prepared as a 0.04% aqueous solution. Besides its primary function as an indicator, bromocresol purple is used in medical laboratories to measure albumin.
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Common stock solution preparation
Table 1. Volume of Water needed to reconstitute specific mass of Bromocresol purple *CAS 115-40-2* *Ultrapure grade* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 185.11 µL | 925.549 µL | 1.851 mL | 9.255 mL | 18.511 mL |
| 5 mM | 37.022 µL | 185.11 µL | 370.22 µL | 1.851 mL | 3.702 mL |
| 10 mM | 18.511 µL | 92.555 µL | 185.11 µL | 925.549 µL | 1.851 mL |
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References
View all 32 references: Citation Explorer
Albumin concentration determined by the modified bromocresol purple method is superior to that by the bromocresol green method for assessing nutritional status in malnourished patients with inflammation
Authors: Ueno T, Hirayama S, Ito M, Nishioka E, Fukushima Y, Satoh T, Idei M, Horiuchi Y, Shoji H, Ohmura H, Shimizu T, Miida T.
Journal: Ann Clin Biochem (2013): 576
Authors: Ueno T, Hirayama S, Ito M, Nishioka E, Fukushima Y, Satoh T, Idei M, Horiuchi Y, Shoji H, Ohmura H, Shimizu T, Miida T.
Journal: Ann Clin Biochem (2013): 576
Using polyvinyl chloride dyed with bromocresol purple in radiation dosimetry
Authors: Kattan M, al Kassiri H, Daher Y.
Journal: Appl Radiat Isot (2011): 377
Authors: Kattan M, al Kassiri H, Daher Y.
Journal: Appl Radiat Isot (2011): 377
Serum globulins contribute to the discrepancies observed between the bromocresol green and bromocresol purple assays of serum albumin concentration
Authors: Xu Y, Wang L, Wang J, Liang H, Jiang X.
Journal: Br J Biomed Sci (2011): 120
Authors: Xu Y, Wang L, Wang J, Liang H, Jiang X.
Journal: Br J Biomed Sci (2011): 120
Mechanism for the color change in bromocresol purple bound to human serum albumin
Authors: Ito S, Yamamoto D.
Journal: Clin Chim Acta (2010): 294
Authors: Ito S, Yamamoto D.
Journal: Clin Chim Acta (2010): 294
Identification of two bromocresol purple binding sites on human serum albumin
Authors: Ito S, Yamamoto D.
Journal: Clin Chim Acta (2010): 1536
Authors: Ito S, Yamamoto D.
Journal: Clin Chim Acta (2010): 1536