Name: Buccutite™ Peroxidase (HRP) Antibody Conjugation Kit *Optimized for Labeling 100 ug Protein*
Brand: AAT Bioquest
SKU: 5503
Price: 277 USD
Availability: InStock

Buccutite™ Peroxidase (HRP) Antibody Conjugation Kit

Optimized for Labeling 100 ug Protein

Protein-protein conjugations are commonly performed with a bifunctional linker (such as the commonly used SMCC), having different reactivity on each end for linking two different proteins. One end of the crosslinker reacts (via NHS ester) with amines (-NH2) found in the amino acid lysine and N-terminus, and the other end reacts (via maleimide) with the thiol groups (-SH) found in the amino acid cysteine. However, SMCC-modified protein is extremely unstable and often self-reactive since proteins often contain both amine and thiol groups that cause significant amount of homo-crosslinking. In addition it is quite difficult and tedious to quantify the number of maleimide groups on a protein. Buccutite™ Peroxidase (HRP) Antibody Conjugation Kit is designed for preparing horseradish peroxidase (HRP) conjugates directly from proteins, peptides, and other ligands that contain a free amino group. The HRP provided in our kit has been pre-activated with our proprietary linker Buccutite™ FOL, and can be directly used for conjugation. The Buccutite™ FOL-activated HRP readily reacts with Buccutite™ MTA-containing molecules under extremely mild neutral conditions without any catalyst required. Compared to commonly used SMCC and other similar technologies, our Buccutite™ bioconjugation system is much more robust and easier to use. It enables faster and quantitative conjugation of biomolecules with higher efficiencies and yields.

The mechanism of Buccutite™ bioconjugation system used for Buccutite™ Peroxidase Antibody Conjugation Kit (Cat# 5503).

Protocol

SDS

COA

Catalog	Size	Price	Quantity
5503	2 Labelings	Price

References

View all 9 references: Citation Explorer

Comparative activity of peroxidase-antibody conjugates with periodate and glutaraldehyde coupling according to an enzyme immunoassay

Authors:

Tresca JP, Ricoux R, Pontet M, Engler R.

Journal:

Ann Biol Clin (Paris) (1995): 227

Influence of the antibody-peroxidase coupling methods on the conjugate stability and on the methodologies for the preservation of the activity in time

Authors:

Presentini R, Terrana B.

Journal:

J Immunoassay (1995): 309

Peroxidase labelled monoclonal antibody against light chains of human cardiac myosin

Authors:

Strakova Z, Barancik M, Lukacova D, Angyal R, Slosarcikova L, Horakova K.

Journal:

Gen Physiol Biophys (1991): 63

Highly efficient and simple methods for the preparation of peroxidase and active peroxidase-antibody conjugates for enzyme immunoassays

Authors:

Tijssen P, Kurstak E.

Journal:

Anal Biochem (1984): 451

Direct immunocytochemistry with a horseradish peroxidase-conjugated monoclonal antibody against substance P

Authors:

Boorsma DM, Cuello AC, van Leeuwen FW.

Journal:

J Histochem Cytochem (1982): 1211

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
UNSPSC	12352200

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Page updated on September 25, 2025

Component A: Buccutite™ FOL-Activated HRP	2 vials (lyophilized)
Component B: Buccutite™ MTA	2 vials (lyophilized)
Component C: Reaction Buffer	1 vial (20 µL)