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Cal-630™-Dextran Conjugate *MW 10,000*

Calcium measurement is critical for numerous biological investigations. Fluorescent probes that show spectral responses upon binding calcium have enabled researchers to investigate changes in intracellular free calcium concentrations by using fluorescence microscopy, flow cytometry, fluorescence spectroscopy and fluorescence microplate readers. Cells may be physically loaded with the cell-impermeant salt forms of these dextran-conjugated calcium indicators using patch pipette or microinjection. The fluorescence signal from these cells is measured using fluorescence microscopy. The dextran forms of our calcium indicators show a dramatic reduction in both leakage and compartmentalization compared to the AM ester forms. Among the fluorescent calcium indicator dextran conjugates, Cal-630 dextran conjugates might be a better choice than other red fluorescent dextrtan conjugates due to its higher fluorescence quantum yield and larger fluorescence enhancement by calcium.

Spectrum

References

View all 32 references: Citation Explorer
High Affinity Mannotetraose as an Alternative to Dextran in ConA Based Fluorescent Affinity Glucose Assay Due to Improved FRET Efficiency
Authors: Locke, A. K.; Cummins, B. M.; Cote, G. L.
Journal: ACS Sens (2016): 584-590
Synthesis and Cell Imaging of a Near-Infrared Fluorescent Magnetic "CdHgTe-Dextran-Magnetic Layered Double Hydroxide-Fluorouracil" Composite
Authors: Jin, X.; Zhang, M.; Gou, G.; Ren, J.
Journal: J Pharm Sci (2016): 1751-61
The effect of the size of fluorescent dextran on its endocytic pathway
Authors: Li, L.; Wan, T.; Wan, M.; Liu, B.; Cheng, R.; Zhang, R.
Journal: Cell Biol Int (2015): 531-9
Characterization of biomimetic calcium phosphate labeled with fluorescent dextran for quantification of osteoclastic activity
Authors: Maria, S. M.; Prukner, C.; Sheikh, Z.; Muller, F. A.; Komarova, S. V.; Barralet, J. E.
Journal: Acta Biomater (2015): 140-6
Visualizing Lysosomal Membrane Permeabilization by Fluorescent Dextran Release
Authors: Ellegaard, A. M.; Jaattela, M.; Nyl and sted, J.
Journal: Cold Spring Harb Protoc (2015): 900-3
Page updated on October 28, 2024

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Catalog Number20546
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Physical properties

Molecular weight

~11000

Solvent

Water

Spectral properties

Excitation (nm)

609

Emission (nm)

626

Quantum yield

0.371

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12352200