Name: Cal-630™, sodium salt
Brand: AAT Bioquest
SKU: 20535
Price: 232 USD
Availability: InStock

Cal-630™, sodium salt

Calcium measurement is critical for numerous biological investigations. Fluorescent probes that show spectral responses upon binding calcium have enabled researchers to investigate changes in intracellular free calcium concentrations by using fluorescence microscopy, flow cytometry, fluorescence spectroscopy and fluorescence microplate readers. x-Rhod-1 is commonly used as a red fluorescent calcium indicator. However, x-Rhod-1 is only moderately fluorescent in live cells upon esterase hydrolysis, and has very small cellular calcium responses. Cal-630™ has been developed to improve x-Rhod-1 cell loading and calcium response while maintaining the spectral wavelength of x-Rhod-1, making it compatible with Texas Red® filter set. In CHO and HEK cells Cal-630™ AM has cellular calcium response that is much more sensitive than x-Rhod-1. The spectra of Cal-630 is well separated from those of FITC, Alexa Fluor® 488 and GFP, making it an ideal calcium probe for multiplexing intracellular assays with GFP cell lines or FITC/Alexa Fluor® 488 labeled antibodies.

Ca2+ Dependent Dose Response of Cal-630.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
20535	5x50 ug	Price

Calculators

Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of Cal-630™, sodium salt to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	95.928 µL	479.639 µL	959.279 µL	4.796 mL	9.593 mL
5 mM	19.186 µL	95.928 µL	191.856 µL	959.279 µL	1.919 mL
10 mM	9.593 µL	47.964 µL	95.928 µL	479.639 µL	959.279 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	1042.45 g/mol	=	mL	x	mM	=	mmol

References

View all 8 references: Citation Explorer

Protein kinase C and myocardial calcium handling during ischemia and reperfusion: lessons learned using Rhod-2 spectrofluorometry

Authors:

Stamm C, del Nido PJ.

Journal:

Thorac Cardiovasc Surg (2004): 127

Cytosolic calcium in the ischemic rabbit heart: assessment by pH- and temperature-adjusted rhod-2 spectrofluorometry

Authors:

Stamm C, Friehs I, Choi YH, Zurakowski D, McGowan FX, del Nido PJ.

Journal:

Cardiovasc Res (2003): 695

Rhod-2 based measurements of intracellular calcium in the perfused mouse heart: cellular and subcellular localization and response to positive inotropy

Authors:

MacGowan GA, Du C, Glonty V, Suhan JP, Koretsky AP, Farkas DL.

Journal:

J Biomed Opt (2001): 23

Changes in mitochondrial Ca2+ detected with Rhod-2 in single frog and mouse skeletal muscle fibres during and after repeated tetanic contractions

Authors:

Lannergren J, Westerblad H, Bruton JD.

Journal:

J Muscle Res Cell Motil (2001): 265

Calibration of the calcium dissociation constant of Rhod(2)in the perfused mouse heart using manganese quenching

Authors:

Du C, MacGowan GA, Farkas DL, Koretsky AP.

Journal:

Cell Calcium (2001): 217

Physical properties

Dissociation constant (K_d, nM)	792
Molecular weight	1042.45
Solvent	Water

Spectral properties

Excitation (nm)	609
Emission (nm)	626
Quantum yield	0.37 ¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200

Documents

Protocol
Safety Data Sheet
Certificate of Analysis

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Page updated on October 26, 2025