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Calcein Deep Red™
Calcein AM is one the most popular fluorescent probes used for labeling and monitoring cellular functions of live cells. However, the single color of Calcein AM makes it impossible to use this valuable reagent in the multicolor applications. For example, it is impossible to use Calcein AM in GFP-tranfacted cells due to the same color to GFP. To address this color limitation of Calcein AM, we have developed Calcein Orange™, Calcein Red™ and Calcein Deep Red™. These new Calcein AM analogs enable the multicolor labeling and functional analysis of live cells in combination with Calcein AM. Calcein Deep Red™ is the esterase-hydrolyzed product of Calcein Deep Red™ acetate in cells. Calcein Deep Red™ dye can be monitored with the common Cy5 filter set. It is used as a fluorescence reference standard for Calcein Deep Red™ acetate.
Images of Live HeLa cells stained with Calcein Deep Red™ (Cat#21902) and Calcein Deep Red™ AM(Cat.22011 ). Calcein Deep Red™ cannot  permeate intact live cells.
Images of Live HeLa cells stained with Calcein Deep Red™ (Cat#21902) and Calcein Deep Red™ AM(Cat.22011 ). Calcein Deep Red™ cannot  permeate intact live cells.
CatalogSize
Price
Quantity
219021 mg
Price
 
Physical properties

Molecular weight373.66
SolventDMSO
Spectral properties

Excitation (nm)643
Emission (nm)663
Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12352200
Instrument settings

Flow cytometer
Excitation640 nm laser
Emission660/20 nm filter
Instrument specification(s)APC channel

Fluorescence microscope
ExcitationCy5 filter set
EmissionCy5 filter set
Recommended plateBlack wall/clear bottom

Fluorescence microplate reader
Excitation620
Emission660
Cutoff630
Recommended plateSolid black
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Emailsales@aatbio.com
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Page updated on January 23, 2025