Calcein | AAT Bioquest

Calcein Deep Red™

Calcein Deep Red™ is a membrane-impermeant fluorescent dye designed for use as a fluorescence reference standard, assay control, and calibration reagent.

Membrane impermeant: Cannot cross intact cell membranes unlike the AM forms
Reference standard: Suitable for quantification and standard curve generation in assays using Calcein Deep Red™ AM
Calibration compatible: Excitation/emission maxima of ~643/663 nm, compatible with standard Cy5 filter sets
Control reagent: Useful for membrane integrity, cell permeabilization, and liposome entrapment studies

Images of Live HeLa cells stained with Calcein Deep Red™ (Cat#21902) and Calcein Deep Red™ AM(Cat.22011 ). Calcein Deep Red™ cannot  permeate intact live cells.

Datasheet

SDS

Catalog	Size	Price	Quantity
21902	1 mg	Price

Overview

Calcein Deep Red™ is a membrane-impermeant fluorescent dye designed specifically for use as a fluorescence reference standard. Unlike acetoxymethyl (AM) ester derivatives, Calcein Deep Red™ cannot readily cross intact cell membranes and therefore is not intended for live-cell loading or intracellular staining applications. The dye exhibits excitation and emission maxima of approximately 643 nm and 663 nm, respectively, making it compatible with standard Cy5 fluorescence detection settings.

Calcein Deep Red™ is primarily used as a fluorescence reference standard for assays employing Calcein Deep Red™ AM and related cell-permeant derivatives. It is also useful as a control reagent for evaluating membrane integrity, cell permeabilization, and liposome encapsulation or leakage. In addition, the dye can be used for fluorescence instrument calibration and assay standardization in red and near-infrared fluorescence detection workflows.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Calcein Deep Red™ to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	267.623 µL	1.338 mL	2.676 mL	13.381 mL	26.762 mL
5 mM	53.525 µL	267.623 µL	535.246 µL	2.676 mL	5.352 mL
10 mM	26.762 µL	133.811 µL	267.623 µL	1.338 mL	2.676 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	373.66 g/mol	=	mL	x	mM	=	mmol

Citations

View all 20 citations: Citation Explorer

Maternal milk cell components are uptaken by infant liver macrophages via extracellular vesicle mediated transport

Authors:

Doerfler, Rose and Yerneni, Saigopalakrishna and LoPresti, Samuel and Chaudhary, Namit and Newby, Alexandra and Melamed, Jilian R and Malaney, Angela and Whitehead, Kathryn A

Journal:

The FASEB Journal (2025): e70340

Skin-Targeted Delivery of Extracellular Vesicle-Encapsulated Curcumin Using Dissolvable Microneedle Arrays

Authors:

Yerneni, Saigopalakrishna S and Yalcintas, Ezgi P and Smith, Jason D and Averick, Saadyah and Campbell, Phil G and Ozdoganlar, O Burak

Journal:

Acta Biomaterialia (2022)

Radioiodination of extravesicular surface constituents to study the biocorona, cell trafficking and storage stability of extracellular vesicles

Authors:

Yerneni, Saigopalakrishna S and Solomon, Talia and Smith, Jason and Campbell, Phil G

Journal:

Biochimica et Biophysica Acta (BBA)-General Subjects (2022): 130069

Functional imaging of neuronal activity of auditory cortex by using Cal-520 in anesthetized and awake mice

Authors:

Li, Jingcheng and Zhang, Jianxiong and Wang, Meng and Pan, Junxia and Chen, Xiaowei and Liao, Xiang

Journal:

Biomedical Optics Express (2017): 2599--2610

NINJ2--A novel regulator of endothelial inflammation and activation

Authors:

Wang, Jingjing and Fa, Jingjing and Wang, Pengyun and Jia, Xinzhen and Peng, Huixin and Chen, Jing and Wang, Yifan and Wang, Chenhui and Chen, Qiuyun and Tu, Xin and others, undefined

Journal:

Cellular Signalling (2017)

References

View all 84 references: Citation Explorer

Functional evidence that the self-renewal gene NANOG regulates esophageal squamous cancer development

Authors:

Li, Deng and Xiang, Xiaocong and Yang, Fei and Xiao, Dongqin and Liu, Kang and Chen, Zhu and Zhang, Ruolan and Feng, Gang

Journal:

Biochemical and Biophysical Research Communications (2017)

Localized functional chemical stimulation of TE 671 cells cultured on nanoporous membrane by calcein and acetylcholine

Authors:

Zibek S, Stett A, Koltay P, Hu M, Zengerle R, Nisch W, Stelzle M.

Journal:

Biophys J. (2006)

A vaccination and challenge model using calcein marked fish

Authors:

Klesius PH, Evans JJ, Shoemaker CA, Pasnik DJ.

Journal:

Fish Shellfish Immunol (2006): 20

Novel fluorescence assay using calcein-AM for the determination of human erythrocyte viability and aging

Authors:

Bratosin D, Mitrofan L, Palii C, Estaquier J, Montreuil J.

Journal:

Cytometry A (2005): 78

Cytotoxic effects of 100 reference compounds on Hep G2 and HeLa cells and of 60 compounds on ECC-1 and CHO cells. I mechanistic assays on ROS, glutathione depletion and calcein uptake

Authors:

Schoonen WG, Westerink WM, de Roos JA, Debiton E.

Journal:

Toxicol In Vitro (2005): 505

Physical properties

Molecular weight	373.66
Solvent	DMSO

Spectral properties

Excitation (nm)	643
Emission (nm)	663

Usage and storage

Intended use	Research Use Only (RUO)
Storage	Freeze (< -15 °C); Minimize light exposure

Instrument settings

Flow cytometer
Excitation	640 nm laser
Emission	660/20 nm filter
Instrument specification(s)	APC channel

Fluorescence microscope
Excitation	Cy5 filter set
Emission	Cy5 filter set
Recommended plate	Black wall/clear bottom

Fluorescence microplate reader
Excitation	620
Emission	660
Cutoff	630
Recommended plate	Solid black

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Fax
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Bulk request	Inquire
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Technical Support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
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Page updated on January 23, 2025