Casein, FITC-conjugated

Casein is considered to be a generic substrate for a broad spectrum of proteases. As native casein this fluoresceinated casein is hydrolyzed by many proteases, and widely used for fluorimetric measurement of protease activity. In the intact substrate, casein is heavily labeled with 5-FITC, resulting in significant fluorescence quenching. Protease-catalyzed hydrolysis relieves its quenching effect, yielding brightly green fluorescent dye-labeled short peptides. The increase in fluorescence intensity is directly proportional to protease activity. We do not recommend that this conjugate be used for fluorescence polarization assay. For fluorescence polarization we can custom-make the lightly labeled fluorescein casein conjugate.

<p>Proteases hydrolyze the quenching effect of the labeled 5-FITC, resulting in a bright green fluorescence proportional to protease activity.</p>

Protocol

SDS

Catalog	Size	Price	Quantity
13440	5 mg	Price

Citations

View all 6 citations: Citation Explorer

Mutations in ClpC1 or ClpX subunit of caseinolytic protease confer resistance to ilamycins in mycobacteria

Authors:

Gao, Yamin and Fang, Cuiting and Zhou, Biao and Hameed, HM Adnan and Sun, Changli and Tian, Xirong and He, Jing and Han, Xingli and Zhang, Han and Li, Jun and others,

Journal:

Communications Biology (2025): 1219

Development of novel imipridone derivatives with potent anti-cancer activities as human caseinolytic peptidase P (hClpP) activators

Authors:

Zhang, Yanzhi and Jiang, Jinxin and Ding, Hao and Li, Qiannan and Xiao, Yibei and Sun, Haiying

Journal:

Bioorganic Chemistry (2024): 107765

Liquid temperature measurement method in microchannels by using fluorescence polarization

Authors:

Tatsumi, Kazuya and Hsu, Chi Hsuan and Suzuki, Atsushi and Nakabe, Kazuyoshi

Journal:

Heat and Mass Transfer (2018): 2607--2616

Liquid temperature measurement method in microchannels by using fluorescence polarization

Authors:

Tatsumi, Kazuya and Hsu, Chi Hsuan and Suzuki, Atsushi and Nakabe, Kazuyoshi

Journal:

Heat and Mass Transfer (2017): 1--10

Micro-scale temperature measurement method using fluorescence polarization

Authors:

Tatsumi, K and Hsu, CH and Suzuki, A and Nakabe, K

References

View all 30 references: Citation Explorer

Transient kinetic experiments demonstrate the existence of a unique catalytic enzyme form in the peptide-stimulated ATPase mechanism of Escherichia coli Lon protease

Authors:

Vineyard D, Zhang X, Lee I.

Journal:

Biochemistry (2006): 11432

Highly stable glycosylated serine protease from the medicinal plant Euphorbia milii

Authors:

Yadav SC, P and e M, Jagannadham MV.

Journal:

Phytochemistry (2006): 1414

Effects of Pseudomonas fluorescens M3/6 bacterial protease on plasmin system and plasminogen activation

Authors:

Frohbieter KA, Ismail B, Nielsen SS, Hayes KD.

Journal:

J Dairy Sci (2005): 3392

Fibrillar amyloid beta-protein inhibits the activity of high molecular weight brain protease and trypsin

Authors:

Chauhan V, Sheikh AM, Chauhan A, Spivack WD, Fenko MD, Malik MN.

Journal:

J Alzheimers Dis (2005): 37

Characterization of a novel and specific inhibitor for the pro-apoptotic protease Omi/HtrA2

Authors:

Cilenti L, Lee Y, Hess S, Srinivasula S, Park KM, Junqueira D, Davis H, Bonventre JV, Alnemri ES, Zervos AS.

Journal:

J Biol Chem (2003): 11489

Physical properties

Molecular weight	N/A
Solvent	Water

Spectral properties

Correction factor (280 nm)	0.35
Extinction coefficient (cm ^-1 M ^-1)	73000
Excitation (nm)	491
Emission (nm)	516
Quantum yield	0.92

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200

Documents

Protocol
Safety Data Sheet
Certificate of Analysis
Brochure: Enzyme Probes & Assay Kits

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Page updated on November 3, 2025