Casein, TAMRA-conjugated

Casein is considered to be a generic substrate for a broad spectrum of proteases. As native casein this rhodaminated casein is hydrolyzed by many proteases, and widely used for fluorometric measurement of protease activity. In the intact substrate, casein is heavily labeled with TAMRA, resulting in significant fluorescence quenching. Protease-catalyzed hydrolysis relieves its quenching effect, yielding brightly orange fluorescent dye-labeled short peptides. The increase in fluorescence intensity is directly proportional to protease activity. Compared to FITC-labeled casein, this casein substrate has pH-independent fluorescence. This feature is more convenient for the assays that require low pH. We do not recommend that this conjugate be used for fluorescence polarization assay. For fluorescence polarization we can custom-make the lightly labeled fluorescein casein conjugate.

<p>Proteases hydrolyze the quenching effect of the labeled TAMRA, resulting in a bright yellow-orange fluorescence proportional to protease activity.</p>

Protocol

SDS

COA

Catalog	Size	Price	Quantity
13441	5 mg	Price

Citations

View all 3 citations: Citation Explorer

Liquid temperature measurement method in microchannels by using fluorescence polarization

Authors:

Tatsumi, Kazuya and Hsu, Chi Hsuan and Suzuki, Atsushi and Nakabe, Kazuyoshi

Journal:

Heat and Mass Transfer (2017): 1--10

Micro-scale temperature measurement method using fluorescence polarization

Authors:

Tatsumi, K and Hsu, CH and Suzuki, A and Nakabe, K

Microscopic Fluid Temperature Measurements Using Fluorescence Polarization Method

Authors:

Tatsumi, Kazuya and Tozaki, Akihisa and Nakabe, Kazuyoshi

References

View all 30 references: Citation Explorer

Transient kinetic experiments demonstrate the existence of a unique catalytic enzyme form in the peptide-stimulated ATPase mechanism of Escherichia coli Lon protease

Authors:

Vineyard D, Zhang X, Lee I.

Journal:

Biochemistry (2006): 11432

Highly stable glycosylated serine protease from the medicinal plant Euphorbia milii

Authors:

Yadav SC, P and e M, Jagannadham MV.

Journal:

Phytochemistry (2006): 1414

Effects of Pseudomonas fluorescens M3/6 bacterial protease on plasmin system and plasminogen activation

Authors:

Frohbieter KA, Ismail B, Nielsen SS, Hayes KD.

Journal:

J Dairy Sci (2005): 3392

Fibrillar amyloid beta-protein inhibits the activity of high molecular weight brain protease and trypsin

Authors:

Chauhan V, Sheikh AM, Chauhan A, Spivack WD, Fenko MD, Malik MN.

Journal:

J Alzheimers Dis (2005): 37

Characterization of a novel and specific inhibitor for the pro-apoptotic protease Omi/HtrA2

Authors:

Cilenti L, Lee Y, Hess S, Srinivasula S, Park KM, Junqueira D, Davis H, Bonventre JV, Alnemri ES, Zervos AS.

Journal:

J Biol Chem (2003): 11489

Physical properties

Molecular weight	N/A
Solvent	Water

Spectral properties

Correction factor (260 nm)	0.32
Correction factor (280 nm)	0.178
Extinction coefficient (cm ^-1 M ^-1)	90000
Excitation (nm)	552
Emission (nm)	578

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical Support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on October 13, 2025