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Cell Meter™ Caspase 9 Activity Apoptosis Assay Kit
Green Fluorescence
Our Cell Meter™ assay kits are a set of tools for monitoring cellular functions. There are a variety of parameters that can be used. This particular kit is designed to monitor cell apoptosis by measuring caspase 9 activity. Caspase 9 is a member of the CED-3 subfamily. Activated Caspase-9 cleaves downstream caspases such as caspase-3, -6 and -7, initiating the caspase cascade. It is essential for apoptosis during normal development of the central nervous system. Caspase 9 is proven to have selectivity for the peptide sequence Leu-Glu-His-Asp (LEHD). This kit uses (Ac-LEHD)2-R110 as a fluorogenic indicator for caspase 9 activity. Cleavage of R110 peptides by caspase 9 generates strongly fluorescent rhodamine 110 (R110)which is monitored at the emission between 520 nm and 530 nm with the excitation between 480 nm and 500 nm. The kit provides all the essential components. The assay is robust and can be readily adapted for high throughput screening. It can be used to either quantify the activated caspase 9 activities in apoptotic cells or screen the caspase 9 inhibitors. Quite a few labs have used this kit for high throughput screenings.
Detection of caspase 9 activities using Cell Meter™ Caspase 9 Activity Apoptosis Assay Kit in Jurkat cells. Jurkat cells were seeded at 200,000 cells/90 µL/well in a Costar black wall/clear bottom 96-well plate. The cells were treated with 10 mM adenosine for 48 hours while the untreated cells were used as control. The caspase 9 Substrate working solution (100 µL/well) was added and incubated at room temperature for 1 hour. The fluorescence intensity was measured at Ex/Em = 490/525 nm with a FlexStation™ microplate reader (Molecular Devices).
Detection of caspase 9 activities using Cell Meter™ Caspase 9 Activity Apoptosis Assay Kit in Jurkat cells. Jurkat cells were seeded at 200,000 cells/90 µL/well in a Costar black wall/clear bottom 96-well plate. The cells were treated with 10 mM adenosine for 48 hours while the untreated cells were used as control. The caspase 9 Substrate working solution (100 µL/well) was added and incubated at room temperature for 1 hour. The fluorescence intensity was measured at Ex/Em = 490/525 nm with a FlexStation™ microplate reader (Molecular Devices).
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
22799200 Tests
Price
 
Spectral properties
Extinction coefficient (cm -1 M -1)
80000
Excitation (nm)500
Emission (nm)522
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12352200
Instrument settings
Fluorescence microplate reader
Excitation490 nm
Emission525 nm
Cutoff515 nm
Recommended plateBlack wall/clear bottom
Instrument specification(s)Top/Bottom read mode
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Page updated on September 25, 2025