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Cell Meter™ Cell Adhesion Assay Kit
The Cell Meter™ Cell Adhesion Assay Kit is a fast and sensitive assay for measuring cell-cell or cell-surface adhesion for a variety of cell types. In this assay, cells are labeled with Calcein UltraGreen AM and allowed to adhere. After removal of nonadherent cells, The fluorescence of Calcein UltraGreen is used to calculate the number of adherent cells. The use of our outstanding fluorogenic dye, Calcein UltraGreen AM provides a fast and sensitive method for measuring cell adhesion with a variety of cell types. Calcein UltraGreen AM is nonfluorescent but, once loaded into cells, is cleaved by endogenous esterases to produce highly fluorescent Calcein UltraGreen, a brightly fluorescent, pH-independent, cytoplasmic cell marker with the minimal interference to cell adhesion process. The Cell Meter™ cell adhesion assay is designed for use with fluorescence microplate readers. The robust performance of Calcein UltraGreen AM and simple procedure of the kit avoids problems associated with assays that utilize radioisotopes, which generate hazardous waste, and with assays that rely on the use of covalently coupled cell-surface labels, which can potentially alter cell function.
Cell adhesion measured with Cell Meter™ Cell Adhesion Assay Kit using a fluorescence microplate reader. Jurkat cells at different confluences or confluency levels were incubated in wells coated with different materials, and then stained with Calcein Ultragreen AM at 37°C for 30 mins. The signal was monitored at Ex/Em = 490/525 nm.
Cell adhesion measured with Cell Meter™ Cell Adhesion Assay Kit using a fluorescence microplate reader. Jurkat cells at different confluences or confluency levels were incubated in wells coated with different materials, and then stained with Calcein Ultragreen AM at 37°C for 30 mins. The signal was monitored at Ex/Em = 490/525 nm.
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
23010100 Tests
Price
 
Spectral properties
Excitation (nm)494
Emission (nm)514
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12171501
Instrument settings
Fluorescence microscope
ExcitationFITC filter set
EmissionFITC filter set
Recommended plateBlack wall/clear bottom
Fluorescence microplate reader
Excitation490 nm
Emission525 nm
Cutoff515 nm
Recommended plateBlack wall/Clear bottom
Documents
Protocol
Safety Data Sheet
Certificate of Analysis
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Page updated on November 3, 2025