Cell Meter™ Cell Adhesion Assay Kit

The Cell Meter™ Cell Adhesion Assay Kit is a fast and sensitive assay for measuring cell-cell or cell-surface adhesion for a variety of cell types. In this assay, cells are labeled with Calcein UltraGreen AM and allowed to adhere. After removal of nonadherent cells, The fluorescence of Calcein UltraGreen is used to calculate the number of adherent cells. The use of our outstanding fluorogenic dye, Calcein UltraGreen AM provides a fast and sensitive method for measuring cell adhesion with a variety of cell types. Calcein UltraGreen AM is nonfluorescent but, once loaded into cells, is cleaved by endogenous esterases to produce highly fluorescent Calcein UltraGreen, a brightly fluorescent, pH-independent, cytoplasmic cell marker with the minimal interference to cell adhesion process. The Cell Meter™ cell adhesion assay is designed for use with fluorescence microplate readers. The robust performance of Calcein UltraGreen AM and simple procedure of the kit avoids problems associated with assays that utilize radioisotopes, which generate hazardous waste, and with assays that rely on the use of covalently coupled cell-surface labels, which can potentially alter cell function.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
23010	100 Tests	Price

References

View all 50 references: Citation Explorer

The Protein A-mediated binding of Staphylococcus to antibodies in flow cytometric assays and its reduction using FcR blocking reagent.

Authors:

Cronin, Ultan P and Girardeaux, Laura and O'Meara, Elaine and Wilkinson, Martin

Journal:

Applied and environmental microbiology (2020)

Effect of advanced glycation end product on paraoxonase 2 expression: Its impact on endoplasmic reticulum stress and inflammation in HUVECs.

Authors:

Ravi, Ramya and Ragavachetty Nagaraj, Nareshkumar and Subramaniam Rajesh, Bharathidevi

Journal:

Life sciences (2020): 117397

Functionalized Graphene Nanoparticles Induce Human Mesenchymal Stem Cells to Express Distinct Extracellular Matrix Proteins Mediating Osteogenesis.

Authors:

Newby, Steven D and Masi, Tom and Griffin, Christopher D and King, William J and Chipman, Anna and Stephenson, Stacy and Anderson, David E and Biris, Alexandru S and Bourdo, Shawn E and Dhar, Madhu

Journal:

International journal of nanomedicine (2020): 2501-2513

Monitoring kinetics reveals critical parameters of IgA-dependent granulocyte-mediated anti-tumor cell cytotoxicity.

Authors:

Zwick, Anabel and Bernhard, Muriel and Knoerck, Arne and Linxweiler, Maximilian and Schick, Bernhard and Heinzelmann, Joana and Smola, Sigrun and Lohse, Stefan

Journal:

Journal of immunological methods (2019): 112644

CD56 expression in breast cancer induces sensitivity to natural killer-mediated cytotoxicity by enhancing the formation of cytotoxic immunological synapse.

Authors:

Taouk, Ghina and Hussein, Ola and Zekak, Moussa and Abouelghar, Ali and Al-Sarraj, Yasser and Abdelalim, Essam M and Karam, Manale

Journal:

Scientific reports (2019): 8756

Spectral properties

Excitation (nm)	494
Emission (nm)	514

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
UNSPSC	12171501

Instrument settings

Fluorescence microscope
Excitation	FITC filter set
Emission	FITC filter set
Recommended plate	Black wall/clear bottom

Fluorescence microplate reader
Excitation	490 nm
Emission	525 nm
Cutoff	515 nm
Recommended plate	Black wall/Clear bottom

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical Support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on October 2, 2025

Component A: Calcein UltraGreen™ AM	1 vial
Component B: Adhesion Assay Buffer	1 bottle (25 mL)
Component C: DMSO	1 vial (100 µL)