Cell Meter™ Cellular Senescence Activity Assay Kit *Green Fluorescence*
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
Quotation | Request |
International | See distributors |
Shipping | Standard overnight for United States, inquire for international |
Spectral properties
Absorbance (nm) | 487 |
Correction Factor (260 nm) | 0.32 |
Correction Factor (280 nm) | 0.35 |
Extinction coefficient (cm -1 M -1) | 800001 |
Excitation (nm) | 498 |
Emission (nm) | 517 |
Quantum yield | 0.79001, 0.952 |
Storage, safety and handling
Intended use | Research Use Only (RUO) |
Alternative formats
Cell Meter™ Cellular Senescence Activity Assay Kit *Red Fluorescence* |
Related products
Overview | ![]() ![]() |
Absorbance (nm) 487 | Correction Factor (260 nm) 0.32 | Correction Factor (280 nm) 0.35 | Extinction coefficient (cm -1 M -1) 800001 | Excitation (nm) 498 | Emission (nm) 517 | Quantum yield 0.79001, 0.952 |
Cellular Senescence is an irreversible growth arrest triggered in order to prevent growth in DNA damaged cells. Senescence-associated beta-galactosidase (SA-beta-gal) is highly overexpressed in senescent cells and it has been widely used as a senescence marker. X-gal staining, a colorimetric method is widely available and used to detect SA-beta-gal in senescent cells. The color method has some limitations such as requirement of fixation of samples due to the low cell permeability of X-gal, longer staining time and low sensitivity. Cell Meter™ Cellular Senescence Activity Assay Kit uses Xite™ beta-D-galactopyranoside, a fluorogenic beta-Gal substrate that readily enters into live cells, and gets cleaved by SA-β-gal inside cells, generating strong green fluorescence. Unlike cell-impermeable X-Gal substrate, it has excellent cell permeability. Cell Meter™ Cellular Senescence Activity Assay Kit enables users to detect the senescence with higher sensitivity with robust performance. The Xite product is well retained inside the cells, producing a stable signal for fluorescence imaging and flow cytometry analysis.
Platform
Flow cytometer
Excitation | 488 nm laser |
Emission | 530/30 nm filter |
Instrument specification(s) | FITC channel |
Fluorescence microscope
Excitation | FITC filter set |
Emission | FITC filter set |
Recommended plate | Black wall/clear bottom |
Components
Example protocol
AT A GLANCE
Protocol Summary
- Treat samples as desired
- Prepare and add Xite™ beta-D-galactopyranoside working solution to samples
- Incubate samples at 37 °C for 15 to 45 minutes
- Monitor the fluorescence intensity using flow cytometer with 530/30 nm filter (FITC channel)
PREPARATION OF STOCK SOLUTIONS
Unless otherwise noted, all unused stock solutions should be divided into single-use aliquots and stored at -20 °C after preparation. Avoid repeated freeze-thaw cycles.
Xite™ beta-D-galactopyranoside stock solution (100X)
Add 100 uL DMSO (Component C) into Xite™ beta-D-galactopyranoside (Component A) and mix well. Note: Store the unused Xite™ beta-D-galactopyranoside stock solution at -20 °C in single use aliquots.PREPARATION OF WORKING SOLUTION
Xite™ beta-D-galactopyranoside working solution (1X)
Dilute 10 uL of Xite™ beta-D-galactopyranoside stock solution (100X) with 1 mL of Assay Buffer to make Xite™ beta-D-galactopyranoside working solution (1X). Note: Xite™ beta-D-galactopyranoside working solution should be used promptly.SAMPLE EXPERIMENTAL PROTOCOL
Protocol
- Treat your samples as desired.
- Wash the cells with buffer of your choice such as DPBS. Note: For selectively tracking β-Gal in live cells, cells can be treated with Bafilomycin A1 for blocking endogenous β-Gal. Optimum concentration of Bafilomycin A1 may vary on type of cells.
- Add 100 uL Xite™ beta-D-galactopyranoside working solution for 15-45 minutes and incubate the samples at 37°C incubator. Note: Optimal time for incubation needs to be determined carefully.
- Remove the working solution and wash cells with buffer of your choice.
- Resuspend the cells in the Assay Buffer (Component B) and monitor the fluorescence intensity with flow cytometer using 530/30 nm filter (FITC channel) or fluorescence microscope with FITC filter set.
Spectrum
Open in Advanced Spectrum Viewer


Spectral properties
Absorbance (nm) | 487 |
Correction Factor (260 nm) | 0.32 |
Correction Factor (280 nm) | 0.35 |
Extinction coefficient (cm -1 M -1) | 800001 |
Excitation (nm) | 498 |
Emission (nm) | 517 |
Quantum yield | 0.79001, 0.952 |
Product Family
Name | Excitation (nm) | Emission (nm) |
Cell Meter™ Cellular Senescence Activity Assay Kit *Red Fluorescence* | 544 | 567 |
Images

Figure 1. Cellular senescence was measured with Cell Meter™ Cellular Senescence Activity Assay Kit using a NovoCyte Flow Cytometer (ACEA Biosciences). HL-60 cells were incubated with Camptothecin for 6 hours to induce senescence and stained with Xite™ beta-D-galactopyranoside for 30 mins at 37oC. The signal was acquired using FITC channel in ACEA NovoCyte flow cytometer.

Figure 2. Cellular senescence was measured with Cell Meter™ Cellular Senescence Activity Assay Kit using a fluorescence microscope. HeLa cells were incubated with Camptothecin for 6 hours to induce senescence and stained with Xite™ beta-D-galactopyranoside for 30 mins at 37C. The signal was acquired using FITC filter set.
Citations
View all 46 citations: Citation Explorer
Merkel Cell Polyomavirus Large T Antigen Induces Cellular Senescence for Host Growth Arrest and Viral Genome Persistence through Its Unique Domain
Authors: Pham, Alexander M and Ortiz, Luz E and Lukacher, Aron E and Kwun, Hyun Jin
Journal: Cells (2023): 380
Authors: Pham, Alexander M and Ortiz, Luz E and Lukacher, Aron E and Kwun, Hyun Jin
Journal: Cells (2023): 380
Topological DNA damage, telomere attrition and T cell senescence during chronic viral infections
Authors: Ji, Y., Dang, X., Nguyen, L. N. T., Nguyen, L. N., Zhao, J., Cao, D., Khanal, S., Schank, M., Wu, X. Y., Morrison, Z. D., Zou, Y., El Gazzar, M., Ning, S., Wang, L., Moorman, J. P., Yao, Z. Q.
Journal: Immun Ageing (2019): 12
Authors: Ji, Y., Dang, X., Nguyen, L. N. T., Nguyen, L. N., Zhao, J., Cao, D., Khanal, S., Schank, M., Wu, X. Y., Morrison, Z. D., Zou, Y., El Gazzar, M., Ning, S., Wang, L., Moorman, J. P., Yao, Z. Q.
Journal: Immun Ageing (2019): 12
Cell senescence, apoptosis and DNA damage cooperate in the remodeling processes accounting for heart morphogenesis
Authors: Lorda-Diez, C. I., Solis-Mancilla, M. E., Sanchez-Fern and ez, C., Garcia-Porrero, J. A., Hurle, J. M., Montero, J. A.
Journal: J Anat (2019): 815-829
Authors: Lorda-Diez, C. I., Solis-Mancilla, M. E., Sanchez-Fern and ez, C., Garcia-Porrero, J. A., Hurle, J. M., Montero, J. A.
Journal: J Anat (2019): 815-829
Dynamic transcriptome profiling in DNA damage-induced cellular senescence and transient cell-cycle arrest
Authors: Zhao, Z., Dong, Q., Liu, X., Wei, L., Liu, L., Li, Y., Wang, X.
Journal: Genomics (2019): ersion="1.0" encoding="UTF-8" ?>23005.enlEndN
Authors: Zhao, Z., Dong, Q., Liu, X., Wei, L., Liu, L., Li, Y., Wang, X.
Journal: Genomics (2019): ersion="1.0" encoding="UTF-8" ?>23005.enlEndN
Stochastic modeling of aging cells reveals how damage accumulation, repair, and cell-division asymmetry affect clonal senescence and population fitness
Authors: Song, R., Acar, M.
Journal: BMC Bioinformatics (2019): 391
Authors: Song, R., Acar, M.
Journal: BMC Bioinformatics (2019): 391
Regulatory T cells trigger effector T cell DNA damage and senescence caused by metabolic competition
Authors: Liu, X., Mo, W., Ye, J., Li, L., Zhang, Y., Hsueh, E. C., Hoft, D. F., Peng, G.
Journal: Nat Commun (2018): 249
Authors: Liu, X., Mo, W., Ye, J., Li, L., Zhang, Y., Hsueh, E. C., Hoft, D. F., Peng, G.
Journal: Nat Commun (2018): 249
The Novel Small Molecule STK899704 Promotes Senescence of the Human A549 NSCLC Cells by Inducing DNA Damage Responses and Cell Cycle Arrest
Authors: Park, C. W., Bak, Y., Kim, M. J., Srinivasrao, G., Hwang, J., Sung, N. K., Kim, B. Y., Yu, J. H., Hong, J. T., Yoon, D. Y.
Journal: Front Pharmacol (2018): 163
Authors: Park, C. W., Bak, Y., Kim, M. J., Srinivasrao, G., Hwang, J., Sung, N. K., Kim, B. Y., Yu, J. H., Hong, J. T., Yoon, D. Y.
Journal: Front Pharmacol (2018): 163
Leucine reduces the proliferation of MC3T3-E1 cells through DNA damage and cell senescence
Authors: da Luz Dias, R., Basso, B., Donadio, M. V. F., Pujol, F. V., Bartrons, R., Haute, G. V., Gassen, R. B., Bregolin, H. D., Krause, G., Viau, C., Saffi, J., Nunes, F. B., Rosa, J. L., de Oliveira, J. R.
Journal: Toxicol In Vitro (2018): 1-10
Authors: da Luz Dias, R., Basso, B., Donadio, M. V. F., Pujol, F. V., Bartrons, R., Haute, G. V., Gassen, R. B., Bregolin, H. D., Krause, G., Viau, C., Saffi, J., Nunes, F. B., Rosa, J. L., de Oliveira, J. R.
Journal: Toxicol In Vitro (2018): 1-10
Hydrogen Treatment Protects against Cell Death and Senescence Induced by Oxidative Damage
Authors: Han, A. L., Park, S. H., Park, M. S.
Journal: J Microbiol Biotechnol (2017): 365-371
Authors: Han, A. L., Park, S. H., Park, M. S.
Journal: J Microbiol Biotechnol (2017): 365-371
MicroRNA-16 feedback loop with p53 and Wip1 can regulate cell fate determination between apoptosis and senescence in DNA damage response
Authors: Issler, M. V. C., Mombach, J. C. M.
Journal: PLoS One (2017): e0185794
Authors: Issler, M. V. C., Mombach, J. C. M.
Journal: PLoS One (2017): e0185794