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Cell Meter™ Cellular Senescence Activity Assay Kit
Red Fluorescence
Cellular Senescence is an irreversible growth arrest triggered in order to prevent growth in DNA damaged cells. Senescence-associated beta-galactosidase is highly overexpressed in senescent cells and has been widely used as a senescence marker. The colorimetric X-gal staining method is widely used to detect SA-beta-gal in senescent cells. However, the color method has some limitations such as the requirement of fixation of cells (due to the low cell permeability of X-gal), longer staining time and low sensitivity. Cell Meter™ Cellular Senescence Activity Assay Kit uses Xite™ Red beta-D-galactopyranoside, a fluorogenic beta-Gal substrate that readily enters into live cells, and gets cleaved by beta-galactosidase inside cells, generating strong red fluorescence. Unlike cell-impermeable X-Gal substrate, it has excellent cell permeability. The robust Cell Meter™ Cellular Senescence Activity Assay Kit enables users to detect the senescence with higher sensitivity. Xite™ Red beta-D-galactopyranoside is fixable for further cell analysis if desired. The red fluorescence of Xite™ Red can be readily combined with other color fluorescent probes such as DAPI or GFP. The Xite Red product is well retained inside cells, producing a stable signal for fluorescence imaging and flow cytometry analysis.
Fixability test with Cell Meter™ Cellular Senescence Activity Assay Kit using a NovoCyte Flow Cytometer (ACEA Biosciences). 9L-LacZ cells were incubated with DMSO or Xite™ Red beta-D-galactopyranoside for 45 mins at 37 °C. The signal before and after fixation was acquired using PE channel. (Cells were then fixed with 4% formaldehyde for 20 minutes at room temperature, and wash once.) 
Fixability test with Cell Meter™ Cellular Senescence Activity Assay Kit using a NovoCyte Flow Cytometer (ACEA Biosciences). 9L-LacZ cells were incubated with DMSO or Xite™ Red beta-D-galactopyranoside for 45 mins at 37 °C. The signal before and after fixation was acquired using PE channel. (Cells were then fixed with 4% formaldehyde for 20 minutes at room temperature, and wash once.) 
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
23007100 Tests
Price
 
Spectral properties
Excitation (nm)544
Emission (nm)567
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12171501
Instrument settings
Flow cytometer
Excitation488 nm laser
Emission575/26 nm filter
Instrument specification(s)PE channel
Fluorescence microscope
ExcitationCy3/TRITC filter set
EmissionCy3/TRITC filter set
Recommended plateBlack wall/clear bottom
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Page updated on October 10, 2025