Name: Cell Meter™ Cellular Senescence Activity Assay Kit *Red Fluorescence*
Brand: AAT Bioquest
SKU: 23007
Price: 396 USD
Availability: InStock

Cell Meter™ Cellular Senescence Activity Assay Kit

Red Fluorescence

Cellular Senescence is an irreversible growth arrest triggered in order to prevent growth in DNA damaged cells. Senescence-associated beta-galactosidase is highly overexpressed in senescent cells and has been widely used as a senescence marker. The colorimetric X-gal staining method is widely used to detect SA-beta-gal in senescent cells. However, the color method has some limitations such as the requirement of fixation of cells (due to the low cell permeability of X-gal), longer staining time and low sensitivity. Cell Meter™ Cellular Senescence Activity Assay Kit uses Xite™ Red beta-D-galactopyranoside, a fluorogenic beta-Gal substrate that readily enters into live cells, and gets cleaved by beta-galactosidase inside cells, generating strong red fluorescence. Unlike cell-impermeable X-Gal substrate, it has excellent cell permeability. The robust Cell Meter™ Cellular Senescence Activity Assay Kit enables users to detect the senescence with higher sensitivity. Xite™ Red beta-D-galactopyranoside is fixable for further cell analysis if desired. The red fluorescence of Xite™ Red can be readily combined with other color fluorescent probes such as DAPI or GFP. The Xite Red product is well retained inside cells, producing a stable signal for fluorescence imaging and flow cytometry analysis.

Fixability test with Cell Meter™ Cellular Senescence Activity Assay Kit using a NovoCyte Flow Cytometer (ACEA Biosciences). 9L-LacZ cells were incubated with DMSO or Xite™ Red beta-D-galactopyranoside for 45 mins at 37 °C. The signal before and after fixation was acquired using PE channel. (Cells were then fixed with 4% formaldehyde for 20 minutes at room temperature, and wash once.)

Protocol

SDS

COA

Catalog	Size	Price	Quantity
23007	100 Tests	Price

Citations

View all 7 citations: Citation Explorer

Sympathetic nerve inhibition enhances calvarial bone repair via senescent macrophage-induced osteogenesis and angiogenesis

Authors:

Zhao, Lei and Xu, Zhaoning and Zhao, Peiru and Pang, Zhiying and Yu, You and Wu, Chao and Zhu, Di and Su, Meiling and Zhang, Ning and Luo, Jian and others,

Journal:

Cell Death Discovery (2025)

CBX7 regulates chemotherapy-induced senescence-like growth arrest in multiple myeloma via the ERK/STAT3/PIM1 axis

Authors:

Ding, Yangyang and Liu, Zelin and Liao, Ya and Huang, Keke and Jiao, Xunyi and Zhu, Jinli and Wang, Huiping and Tao, Qianshan and Guo, Jinjing and Xiong, Shudao and others,

Journal:

Journal of Translational Medicine (2025): 1292

Cell cycle regulator MYBL2 is a distinct vulnerability in acute myeloid leukemia

Authors:

K{\"u}chler, Sandra and Brilloff, Silke and Sch{\"a}fer, Silvia and Rahimian, Elahe and Kufrin, Vida and Peri, Shraddha S and Musa, Julian and Gr{\"u}newald, Thomas GP and Schewe, Denis M and Ball, Claudia R and others,

Journal:

Cell Death Discovery (2025): 470

Extended replicative lifespan of primary resting T cells by CRISPR/dCas9-based epigenetic modifiers and transcriptional activators

Authors:

Huang, Siping and Lau, Cia-Hin and Tin, Chung and Lam, Raymond HW

Journal:

Cellular and Molecular Life Sciences (2024): 1--18

TRAIP resolves DNA replication-transcription conflicts during the S-phase of unperturbed cells

Authors:

Scaramuzza, Shaun and Jones, Rebecca M and Sadurni, Martina Muste and Reynolds-Winczura, Alicja and Poovathumkadavil, Divyasree and Farrell, Abigail and Natsume, Toyoaki and Rojas, Patricia and Cuesta, Cyntia Fernandez and Kanemaki, Masato T and others,

Journal:

Nature Communications (2023): 5071

References

View all 50 references: Citation Explorer

1,8-Cineole promotes G0/G1 cell cycle arrest and oxidative stress-induced senescence in HepG2 cells and sensitizes cells to anti-senescence drugs.

Authors:

Rodenak-Kladniew, Boris and Castro, Agustina and Stärkel, Peter and Galle, Marianela and Crespo, Rosana

Journal:

Life sciences (2020): 117271

Ginsenoside Rg1 Inhibits Cell Proliferation and Induces Markers of Cell Senescence in CD34+CD38- Leukemia Stem Cells Derived from KG1α Acute Myeloid Leukemia Cells by Activating the Sirtuin 1 (SIRT1)/Tuberous Sclerosis Complex 2 (TSC2) Signaling Pathway.

Authors:

Tang, Yan-Long and Zhang, Cheng-Gui and Liu, Heng and Zhou, Yue and Wang, Ya-Ping and Li, Yuan and Han, Yan-Jun and Wang, Cui-Li

Journal:

Medical science monitor : international medical journal of experimental and clinical research (2020): e918207

Integrating physiological and metabolites analysis to identify ethylene involvement in petal senescence in Tulipa gesneriana.

Authors:

Wang, Yaping and Zhao, Huimin and Liu, Chunli and Cui, Guangfen and Qu, Lianwei and Bao, Manzhu and Wang, Jihua and Chan, Zhulong and Wang, Yanping

Journal:

Plant physiology and biochemistry : PPB (2020): 121-131

NAMPT Inhibition Suppresses Cancer Stem-like Cells Associated with Therapy-Induced Senescence in Ovarian Cancer.

Authors:

Nacarelli, Timothy and Fukumoto, Takeshi and Zundell, Joseph A and Fatkhutdinov, Nail and Jean, Stephanie and Cadungog, Mark G and Borowsky, Mark E and Zhang, Rugang

Journal:

Cancer research (2020): 890-900

LPS-induced premature osteocyte senescence: Implications in inflammatory alveolar bone loss and periodontal disease pathogenesis.

Authors:

Aquino-Martinez, Ruben and Rowsey, Jennifer L and Fraser, Daniel G and Eckhardt, Brittany A and Khosla, Sundeep and Farr, Joshua N and Monroe, David G

Journal:

Bone (2020): 115220

Spectral properties

Excitation (nm)	544
Emission (nm)	567

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Flow cytometer
Excitation	488 nm laser
Emission	575/26 nm filter
Instrument specification(s)	PE channel

Fluorescence microscope
Excitation	Cy3/TRITC filter set
Emission	Cy3/TRITC filter set
Recommended plate	Black wall/clear bottom

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical Support	Contact us
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Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on July 17, 2026

Component A: Xite™ Red beta-D-galactopyranoside	1 vial
Component B: Assay Buffer	1 bottle (20 mL)
Component C: DMSO	1 vial (100 μL)