Cell Meter™ Cellular Senescence Activity Assay Kit *Green Fluorescence*

Cell Meter™ Cellular Senescence Activity Assay Kit

Green Fluorescence

Cellular Senescence is an irreversible growth arrest triggered in order to prevent growth in DNA damaged cells. Senescence-associated beta-galactosidase (SA-beta-gal) is highly overexpressed in senescent cells and it has been widely used as a senescence marker. X-gal staining, a colorimetric method is widely available and used to detect SA-beta-gal in senescent cells. The color method has some limitations such as requirement of fixation of samples due to the low cell permeability of X-gal, longer staining time and low sensitivity. Cell Meter™ Cellular Senescence Activity Assay Kit uses Xite™ beta-D-galactopyranoside, a fluorogenic beta-Gal substrate that readily enters into live cells, and gets cleaved by SA-β-gal inside cells, generating strong green fluorescence. Unlike cell-impermeable X-Gal substrate, it has excellent cell permeability. Cell Meter™ Cellular Senescence Activity Assay Kit enables users to detect the senescence with higher sensitivity with robust performance. The Xite product is well retained inside the cells, producing a stable signal for fluorescence imaging and flow cytometry analysis.

Cellular senescence was measured with Cell Meter™ Cellular Senescence Activity Assay Kit using a NovoCyte Flow Cytometer (ACEA Biosciences). HL-60 cells were incubated with Camptothecin for 6 hours to induce senescence and stained with Xite™ beta-D-galactopyranoside for 30 mins at 37<sup>o</sup>C. The signal was acquired using FITC channel in ACEA NovoCyte flow cytometer.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
23005	100 Tests	Price

Citations

View all 47 citations: Citation Explorer

TRAIP resolves DNA replication-transcription conflicts during the S-phase of unperturbed cells

Authors:

Scaramuzza, Shaun and Jones, Rebecca M and Sadurni, Martina Muste and Reynolds-Winczura, Alicja and Poovathumkadavil, Divyasree and Farrell, Abigail and Natsume, Toyoaki and Rojas, Patricia and Cuesta, Cyntia Fernandez and Kanemaki, Masato T and others,

Journal:

Nature Communications (2023): 5071

Merkel Cell Polyomavirus Large T Antigen Induces Cellular Senescence for Host Growth Arrest and Viral Genome Persistence through Its Unique Domain

Authors:

Pham, Alexander M and Ortiz, Luz E and Lukacher, Aron E and Kwun, Hyun Jin

Journal:

Cells (2023): 380

Topological DNA damage, telomere attrition and T cell senescence during chronic viral infections

Authors:

Ji, Y., Dang, X., Nguyen, L. N. T., Nguyen, L. N., Zhao, J., Cao, D., Khanal, S., Schank, M., Wu, X. Y., Morrison, Z. D., Zou, Y., El Gazzar, M., Ning, S., Wang, L., Moorman, J. P., Yao, Z. Q.

Journal:

Immun Ageing (2019): 12

Cell senescence, apoptosis and DNA damage cooperate in the remodeling processes accounting for heart morphogenesis

Authors:

Lorda-Diez, C. I., Solis-Mancilla, M. E., Sanchez-Fern and ez, C., Garcia-Porrero, J. A., Hurle, J. M., Montero, J. A.

Journal:

J Anat (2019): 815-829

Dynamic transcriptome profiling in DNA damage-induced cellular senescence and transient cell-cycle arrest

Authors:

Zhao, Z., Dong, Q., Liu, X., Wei, L., Liu, L., Li, Y., Wang, X.

Journal:

Genomics (2019): ersion="1.0" encoding="UTF-8" ?>23005.enlEndN

Spectral properties

Absorbance (nm)	487
Correction factor (260 nm)	0.32
Correction factor (280 nm)	0.35
Extinction coefficient (cm ^-1 M ^-1)	80000 ¹
Excitation (nm)	498
Emission (nm)	517
Quantum yield	0.7900 ¹ , 0.95 ²

Storage, safety and handling

Intended use

Research Use Only (RUO)

Instrument settings

Flow cytometer
Excitation	488 nm laser
Emission	530/30 nm filter
Instrument specification(s)	FITC channel

Fluorescence microscope
Excitation	FITC filter set
Emission	FITC filter set
Recommended plate	Black wall/clear bottom

Documents

Protocol
Safety Data Sheet
Certificate of Analysis

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Page updated on October 31, 2025

Component A: Xite™ beta-D-galactopyranoside	1 vial
Component B: Assay Buffer	1 bottle (20 mL)
Component C: DMSO	1 vial (100 uL)