logo
Products
Technologies
Applications
Services
Resources
Selection Guides
About
Cell Meter™ Fluorescence Gap Junction Tracing Kit
Gap junctions are specialized intercellular membrane channels constituted with connexin that selectively facilitate the passage of small molecules of <1.5 kD across cells. They are tightly regulated by voltage, growth factors, cAMP, and retinoids, and they are modulated by phosphorylation. Cell Meter™ Fluorescence Gap Junction Tracing Kit provides a reliable and robust assay for the in vitro determination of gap junction function. The method is noninvasive. The cell population under study is divided such that one fraction is loaded with a lipophilic cell plasma membrane permeable dye, Calcein UltraGreen™ AM, that is hydrolyzed upon cellular uptake by cytoplasmic esterases to yield Calcein UltraGreen, a highly fluorescent and well-retained and membrane-impermeable molecule. The other fraction is loaded DiD, which is a lipophilic membrane dye that diffuses laterally to stain the entire cell membrane in deep red fluorescence upon incorporation into membranes. The two fractions are mixed and incubated under coculture conditions. Calcein UltraGreen is transferred to the DiD-stained cells through gap junctions. The assessment of this uptake can be monitored by fluorescence imaging or flow cytometry.
The GAP junctions were analyzed by flow cytometry. HeLa cells were stained with Calcein Ultragreen AM and DiD separately as per the protocol. &nbsp;Cells were mixed well with 1:1 ratio and replated with cell culture medium. Response was measured using NovoCyte flow cytometer (ACEA Biosciences) with FITC and APC channel. As time progress, Q2-2 population (double positive population) increases.
The GAP junctions were analyzed by flow cytometry. HeLa cells were stained with Calcein Ultragreen AM and DiD separately as per the protocol. &nbsp;Cells were mixed well with 1:1 ratio and replated with cell culture medium. Response was measured using NovoCyte flow cytometer (ACEA Biosciences) with FITC and APC channel. As time progress, Q2-2 population (double positive population) increases.
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
23600100 Tests
Price
 
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12171501
Instrument settings
Flow cytometer
Excitation488 nm and 640 nm laser
Emission530/30 nm and 660/20 nm filters
Instrument specification(s)FITC and APC channel
Fluorescence microscope
ExcitationFITC and Cy5 filter sets
EmissionFITC and Cy5 filter sets
Recommended plateBlack wall/clear bottom
Contact us
Telephone
Fax
Emailsales@aatbio.com
InternationalSee distributors
Bulk requestInquire
Custom sizeInquire
Technical SupportContact us
Request quotationRequest
Purchase orderSend to sales@aatbio.com
ShippingStandard overnight for United States, inquire for international
Page updated on September 21, 2025