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Cell Meter™ Fluorimetric Cellular Voltage Assay Kit

The monitoring of membrane potential in HeLa cells using Cell Meter™ Fluorimetric Cellular Voltage Assay kit.  HeLa cells were stained according to the kit instructions, and stimulated with depolarizing solution (164.5 mM KCl, 2 mM CaCl<sub>2</sub>, 1 mM MgCl<sub>2</sub> , 10 mM glucose, 20 mM HEPES, pH 7.4). The response was recorded using FlexStation 3 (Molecular devices).
The monitoring of membrane potential in HeLa cells using Cell Meter™ Fluorimetric Cellular Voltage Assay kit.  HeLa cells were stained according to the kit instructions, and stimulated with depolarizing solution (164.5 mM KCl, 2 mM CaCl<sub>2</sub>, 1 mM MgCl<sub>2</sub> , 10 mM glucose, 20 mM HEPES, pH 7.4). The response was recorded using FlexStation 3 (Molecular devices).
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Catalog Number35000
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Telephone1-408-733-1055
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H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12171501
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OverviewpdfSDSpdfProtocol


Almost all plasma membranes have an electrical potential across them, with the inside usually negative with respect to the outside. Signals are generated by opening or closing of ion channels at one point in the membrane, producing a local change in the membrane potential. This change in the electric field can be quickly affected by either adjacent or more distant ion channels in the membrane. Those ion channels can then open or close as a result of the potential change, reproducing the signal. Cell Meter™ Fluorimetric Cellular Voltage Assay Kit uses a FRET pair (VSB 405 and VSR 555) to monitor changes in cellular membrane potentials. The lipophilic VSB 405 is primarily located on the outer layer of lipid membranes while the localization of VSR 555 is sensitive to cellular membrane potential. At rest state, the inner layer of has a relatively negative potential, making VSR 555 predominantly located in close proximity to the outer layer of cell membranes, thus close to the blue fluorescent VSB405, resulting in efficient fluorescence transfer from blue (VSB 405) to red (VSR 555). When the cell is depolarized, VSR 555 translocates to the inner layer of the cell membrane, thus separating the FRET pair and disrupting FRET. The ratio of blue/red fluorescence is proportional to the cell voltage. This assay can used for screening compounds that modulate ion channels.

Platform


Fluorescence microplate reader

Excitation405 nm
Emission460 nm and 580 nm
Cutoff435 nm and 515 nm
Recommended plateBlack wall/Clear bottom
Instrument specification(s)Bottom read mode/Programmable liquid handling

Other instruments

ArrayScan, FDSS, FLIPR, FlexStation, IN Cell Analyzer, ViewLux

Components


Component A: VSB4051 vial
Component B: 100X Pluronic® F1271 vial (100 µL)
Component C: VSR5551 vial
Component D: Voltage Assay Buffer I1 bottle (50 mL)
Component E: Voltage Assay Buffer II1 bottle (10 mL)
Component F: DMSO1 vial (200 µL)

Citations


View all 2 citations: Citation Explorer
Activation of the LINC00242/miR-141/FOXC1 axis underpins the development of gastric cancer
Authors: Zhong, Xiongdong and Yu, Xianchang and Wen, Xiaoyan and Chen, Lei and Gu, Ni
Journal: Cancer Cell International (2020): 1--11
MicroRNA-1305 inhibits the stemness of LCSCs and tumorigenesis by repressing the UBE2T-dependent Akt-signaling pathway
Authors: Wei, Xiaoyong and You, Xiaolong and Zhang, Jianlong and Zhou, Cuncai
Journal: Molecular Therapy-Nucleic Acids (2019): 721--732

References


View all 12 references: Citation Explorer
Assessing Spatiotemporal and Functional Organization of Mitochondrial Networks.
Authors: Kurz, Felix T and Aon, Miguel A and O'Rourke, Brian and Armoundas, Antonis A
Journal: Methods in molecular biology (Clifton, N.J.) (2018): 383-402
Visualizing and Quantifying the Effect of the Inhibition of HSP70 on Breast Cancer Cells Based on Laser Scanning Microscopy.
Authors: Yu, Biying and Yang, Hongqin and Zhang, Xiaoman and Li, Hui
Journal: Technology in cancer research & treatment (2018): 1533033818785274
Two-photon deep-tissue spatially resolved mitochondrial imaging using membrane potential fluorescence fluctuations.
Authors: Tehrani, Kayvan Forouhesh and Pendleton, Emily G and Southern, William M and Call, Jarrod A and Mortensen, Luke J
Journal: Biomedical optics express (2018): 254-259
Calcineurin/nuclear factor-κB signaling mediates isoflurane-induced hippocampal neuroinflammation and subsequent cognitive impairment in aged rats.
Authors: Li, Zhengqian and Ni, Cheng and Xia, Chun and Jaw, Joey and Wang, Yujie and Cao, Yiyun and Xu, Mao and Guo, Xiangyang
Journal: Molecular medicine reports (2017): 201-209
Amplification of arsenic genotoxicity by TiO2 nanoparticles in mammalian cells: new insights from physicochemical interactions and mitochondria.
Authors: Wang, Xinan and Liu, Yun and Wang, Juan and Nie, Yaguang and Chen, Shaopeng and Hei, Tom K and Deng, Zhaoxiang and Wu, Lijun and Zhao, Guoping and Xu, An
Journal: Nanotoxicology (2017): 978-995
The novel isoxazoline ectoparasiticide fluralaner: selective inhibition of arthropod γ-aminobutyric acid- and L-glutamate-gated chloride channels and insecticidal/acaricidal activity.
Authors: Gassel, Michael and Wolf, Christian and Noack, Sandra and Williams, Heike and Ilg, Thomas
Journal: Insect biochemistry and molecular biology (2014): 111-24
Protective role of mitochondrial K-ATP channel and mitochondrial membrane transport pore in rat kidney ischemic postconditioning.
Authors: Zhang, Wei-liang and Zhao, Yan-li and Liu, Xiao-ming and Chen, Jing and Zhang, Dong
Journal: Chinese medical journal (2011): 2191-5
How DASPMI reveals mitochondrial membrane potential: fluorescence decay kinetics and steady-state anisotropy in living cells.
Authors: Ramadass, Radhan and Bereiter-Hahn, Jürgen
Journal: Biophysical journal (2008): 4068-76
A cell-sparing electric field stimulation technique for high-throughput screening of voltage-gated ion channels.
Authors: Bugianesi, Randal M and Augustine, Paul R and Azer, Karim and Dufresne, Claude and Herrington, James and Kath, Gary S and McManus, Owen B and Napolitano, Christopher S and Rush, Alison and Sachs, Jeff and Simpson, Neal and Wismer, Michael K and Kaczorowski, Gregory J and Slaughter, Robert S
Journal: Assay and drug development technologies (2006): 21-35
Bioassay-guided isolation of epiquinamide, a novel quinolizidine alkaloid and nicotinic agonist from an Ecuadoran poison frog, Epipedobates tricolor.
Authors: Fitch, Richard W and Garraffo, H Martin and Spande, Thomas F and Yeh, Herman J C and Daly, John W
Journal: Journal of natural products (2003): 1345-50