Cell Meter™ Fluorimetric Cellular Voltage Assay Kit
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
UNSPSC | 12171501 |
Related products
Overview | ![]() ![]() |
See also: Plasma Membrane
Almost all plasma membranes have an electrical potential across them, with the inside usually negative with respect to the outside. Signals are generated by opening or closing of ion channels at one point in the membrane, producing a local change in the membrane potential. This change in the electric field can be quickly affected by either adjacent or more distant ion channels in the membrane. Those ion channels can then open or close as a result of the potential change, reproducing the signal. Cell Meter™ Fluorimetric Cellular Voltage Assay Kit uses a FRET pair (VSB 405 and VSR 555) to monitor changes in cellular membrane potentials. The lipophilic VSB 405 is primarily located on the outer layer of lipid membranes while the localization of VSR 555 is sensitive to cellular membrane potential. At rest state, the inner layer of has a relatively negative potential, making VSR 555 predominantly located in close proximity to the outer layer of cell membranes, thus close to the blue fluorescent VSB405, resulting in efficient fluorescence transfer from blue (VSB 405) to red (VSR 555). When the cell is depolarized, VSR 555 translocates to the inner layer of the cell membrane, thus separating the FRET pair and disrupting FRET. The ratio of blue/red fluorescence is proportional to the cell voltage. This assay can used for screening compounds that modulate ion channels.
Platform
Fluorescence microplate reader
Excitation | 405 nm |
Emission | 460 nm and 580 nm |
Cutoff | 435 nm and 515 nm |
Recommended plate | Black wall/Clear bottom |
Instrument specification(s) | Bottom read mode/Programmable liquid handling |
Other instruments
ArrayScan, FDSS, FLIPR, FlexStation, IN Cell Analyzer, ViewLuxComponents
Images

Figure 1. The monitoring of membrane potential in HeLa cells using Cell Meter™ Fluorimetric Cellular Voltage Assay kit. HeLa cells were stained according to the kit instructions, and stimulated with depolarizing solution (164.5 mM KCl, 2 mM CaCl2, 1 mM MgCl2 , 10 mM glucose, 20 mM HEPES, pH 7.4). The response was recorded using FlexStation 3 (Molecular devices).
Citations
View all 2 citations: Citation Explorer
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Authors: Zhong, Xiongdong and Yu, Xianchang and Wen, Xiaoyan and Chen, Lei and Gu, Ni
Journal: Cancer Cell International (2020): 1--11
Authors: Zhong, Xiongdong and Yu, Xianchang and Wen, Xiaoyan and Chen, Lei and Gu, Ni
Journal: Cancer Cell International (2020): 1--11
MicroRNA-1305 inhibits the stemness of LCSCs and tumorigenesis by repressing the UBE2T-dependent Akt-signaling pathway
Authors: Wei, Xiaoyong and You, Xiaolong and Zhang, Jianlong and Zhou, Cuncai
Journal: Molecular Therapy-Nucleic Acids (2019): 721--732
Authors: Wei, Xiaoyong and You, Xiaolong and Zhang, Jianlong and Zhou, Cuncai
Journal: Molecular Therapy-Nucleic Acids (2019): 721--732
References
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