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Cell Meter™ Live Cell Caspase 13 Binding Assay Kit
Green Fluorescence
Our Cell Meter™ live cell caspases activity assay kits are based on fluorescent FMK inhibitors of caspases. These inhibitors are cell permeable and non-cytotoxic. Once inside the cell, the caspase inhibitors bind covalently to the active caspases. This Cell Meter™ Live Cell Caspase 13 Activity Assay Kit is designed to detect cell apoptosis by measuring caspase 13 activation in live cells. It is used for the quantification of activated caspase 13 activities in apoptotic cells, or for screening caspase 13 inhibitors. FAM-LEED-FMK, the green label reagent, allows for direct detection of activated caspase 13 in apoptotic cells by fluorescence microscopy, flow cytometer, or fluorescent microplate reader. The kit provides all the essential components with an optimized assay protocol.
<p>FAM-LEED-FMK fluorometric detection of active caspase 13 using Kit # 20125 in Jurkat cells. The cells were treated with 1 &mu;M staurosporine for 3 hours (Red) while untreated cells were used as a control (Blue). Cells were incubated with FAM-LEED-FMK for 1 hour at 37&deg;C. The fluorescent intensity (300, 000 cells/100 &mu;L/well) was measured at Ex/Em = 490/525 nm (cut off at 515 nm) with a FlexStation microplate reader using bottom read mode.</p>
<p>FAM-LEED-FMK fluorometric detection of active caspase 13 using Kit # 20125 in Jurkat cells. The cells were treated with 1 &mu;M staurosporine for 3 hours (Red) while untreated cells were used as a control (Blue). Cells were incubated with FAM-LEED-FMK for 1 hour at 37&deg;C. The fluorescent intensity (300, 000 cells/100 &mu;L/well) was measured at Ex/Em = 490/525 nm (cut off at 515 nm) with a FlexStation microplate reader using bottom read mode.</p>
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
2012525 Tests
Price
 
Spectral properties
Correction factor (260 nm)0.32
Correction factor (280 nm)0.178
Extinction coefficient (cm -1 M -1)
83000
Excitation (nm)493
Emission (nm)517
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12352200
Instrument settings
Flow cytometer
ExcitationSee Table 1
EmissionSee Table 1
Fluorescence microscope
ExcitationSee Table 1
EmissionSee Table 1
Recommended plateBlack wall/clear bottom
Fluorescence microplate reader
ExcitationSee Table 1
EmissionSee Table 1
Recommended plateBlack wall/clear bottom
Instrument specification(s)Bottom read mode
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Page updated on October 13, 2025