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Cell Meter™ Live Cell Caspase 3/7 Binding Assay Kit
Green Fluorescence
Our Cell Meter™ live cell caspases activity assay kits are based on fluorescent FMK inhibitors of caspases. These inhibitors are cell permeable and non-cytotoxic. Once inside the cell, the caspase inhibitors bind covalently to the active caspases. The activation of caspase 3/7 is important for the initiation of apoptosis. It has been proven that caspase 3/7 has substrate selectivity for the peptide sequence Asp-Glu-Val-Asp (DEVD). This kit uses FAM-DEVD-FMK as a fluorescent indicator for caspase 3/7 activity. FAM-DEVD-FMK irreversibly binds to activated caspase 3/7 in apoptotic cells. Once bound to caspase 3/7, the fluorescent reagent is retained inside the cell. The binding event inhibits caspase 3/7 but will not stop apoptosis from proceeding. There are a variety of parameters that can be used for monitoring cell apoptosis. This Cell Meter™ Live Cell Caspase 3/7 Activity Assay Kit is designed to detect cell apoptosis by measuring caspase 3/7 activation in live cells. It is used for the quantification of activated caspase 3/7 activities in apoptotic cells, or for screening caspase 3/7 inhibitors. FAM-DEVD-FMK, the green label reagent, allows for direct detection of activated caspase 3/7 in apoptotic cells by fluorescence microscopy, flow cytometer, or fluorescent microplate reader. The kit provides all the essential components with an optimized assay protocol.
<p>FAM-DEVD-FMK fluorometric detection of active caspases 3/7 using Kit #20100 in Jurkat cells. The cells were treated with 1 &mu;M staurosporine for 3 hours (Red) while untreated cells were used as a control (Blue). Cells were incubated with FAM-DEVD-FMK for 1 hour at 37&deg;C. The Fluorescent intensity (300, 000 cells/100 &mu;L/well) was measured at Ex/Em = 490/525 nm (cut off at 515 nm) with a FlexStation microplate reader using bottom read mode.</p>
<p>FAM-DEVD-FMK fluorometric detection of active caspases 3/7 using Kit #20100 in Jurkat cells. The cells were treated with 1 &mu;M staurosporine for 3 hours (Red) while untreated cells were used as a control (Blue). Cells were incubated with FAM-DEVD-FMK for 1 hour at 37&deg;C. The Fluorescent intensity (300, 000 cells/100 &mu;L/well) was measured at Ex/Em = 490/525 nm (cut off at 515 nm) with a FlexStation microplate reader using bottom read mode.</p>
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
2010025 Tests
Price
 
Spectral properties
Correction factor (260 nm)0.32
Correction factor (280 nm)0.178
Extinction coefficient (cm -1 M -1)
83000
Excitation (nm)493
Emission (nm)517
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12352200
Instrument settings
Flow cytometer
Instrument specification(s)See Table 1
Fluorescence microscope
Recommended plateBlack wall/clear bottom
Instrument specification(s)See Table 1
Fluorescence microplate reader
Recommended plateSolid black
Instrument specification(s)See Table 2
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Page updated on September 29, 2025