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Cell Meter™ Live Cell Caspase 6 Binding Assay Kit
Green Fluorescence
Our Cell Meter™ live cell caspases activity assay kits are based on fluorescent FMK inhibitors of caspases. These inhibitors are cell permeable and non-cytotoxic. Once inside the cell, the caspase inhibitors bind covalently to the active caspases. This Cell Meter™ Live Cell Caspase 6 Activity Assay Kit is designed to detect cell apoptosis by measuring caspase 6 activation in live cells. It is used for the quantification of activated caspase 6 activities in apoptotic cells, or for screening caspase 6 inhibitors. FAM-VEID-FMK, the green label reagent, allows for direct detection of activated caspase 6 in apoptotic cells by fluorescence microscopy, flow cytometer, or fluorescent microplate reader. The kit provides all the essential components with an optimized assay protocol.
<p>FAM-VEID-FMK fluorometric detection of active Caspase 6 using Kit # 20113 in Jurkat cells. The cells were treated with 1 &mu;M staurosporine for 3 hours (Red) while untreated cells were used as a control (Blue). Cells were incubated with FAM-VEID-FMK for 1 hour at 37&deg;C. The fluorescent intensity (300, 000 cells/100 &mu;L/well) was measured at Ex/Em = 490/525 nm (cut off at 515 nm) with a FlexStation microplate reader using bottom read mode.</p>
<p>FAM-VEID-FMK fluorometric detection of active Caspase 6 using Kit # 20113 in Jurkat cells. The cells were treated with 1 &mu;M staurosporine for 3 hours (Red) while untreated cells were used as a control (Blue). Cells were incubated with FAM-VEID-FMK for 1 hour at 37&deg;C. The fluorescent intensity (300, 000 cells/100 &mu;L/well) was measured at Ex/Em = 490/525 nm (cut off at 515 nm) with a FlexStation microplate reader using bottom read mode.</p>
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
2011325 Tests
Price
 
Spectral properties
Correction factor (260 nm)0.32
Correction factor (280 nm)0.178
Extinction coefficient (cm -1 M -1)
83000
Excitation (nm)493
Emission (nm)517
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12352200
Instrument settings
Flow cytometer
ExcitationSee Table 1
EmissionSee Table 1
Fluorescence microscope
ExcitationSee Table 1
EmissionSee Table 1
Recommended plateBlack wall/clear bottom
Fluorescence microplate reader
ExcitationSee Table 1
EmissionSee Table 1
Recommended plateBlack wall/clear bottom
Instrument specification(s)Bottom read mode
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Page updated on October 13, 2025