Cell Meter™ Live Cell Caspase 8 Binding Assay Kit *Red Fluorescence*

Cell Meter™ Live Cell Caspase 8 Binding Assay Kit

Red Fluorescence

Our Cell Meter™ live cell caspases activity assay kits are based on fluorescent FMK inhibitors of caspases. These inhibitors are cell permeable and non-cytotoxic. Once inside the cell, the caspase inhibitors bind covalently to the active caspases. This Cell Meter™ Live Cell Caspase 8 Activity Assay Kit is designed to detect cell apoptosis by measuring caspase 8 activation in live cells. It is used for the quantification of activated caspase 8 activities in apoptotic cells, or for screening caspase 8 inhibitors. iFluor® 647-LETD-FMK, the red label reagent, allows for direct detection of activated caspase 8 in apoptotic cells by fluorescence microscopy, flow cytometer, or fluorescent microplate reader. The kit provides all the essential components with an optimized assay protocol.

Flow cytometric analysis of active caspase 8 using Cell Meter™ Live Cells Caspase 8 detection kit in Jurkat cells. The cells were treated with 1 μM staurosporine for 5 hours (Green) while untreated cells were used as a control (Red). The staurosporine response was inhibited by Z-VAD-FMK (caspase inhibitor) shown as blue. Cells were incubated with iFluor 647-LETD-FMK for 1 hour at RT. The fluorescent intensity was measured using NovoCyte flow cytometer with 660/20 nm filter (APC channel).

Protocol

SDS

COA

Catalog	Size	Price	Quantity
20116	25 Tests	Price

References

View all 50 references: Citation Explorer

Cigarette smoke inhibits the NLRP3 inflammasome and leads to caspase-1 activation via the TLR4-TRIF-caspase-8 axis in human macrophages.

Authors:

Buscetta, Marco and Di Vincenzo, Serena and Miele, Monica and Badami, Ester and Pace, Elisabetta and Cipollina, Chiara

Journal:

FASEB journal : official publication of the Federation of American Societies for Experimental Biology (2020): 1819-1832

Caspase-8 Induces Lysosome-Associated Cell Death in Cancer Cells.

Authors:

Zhong, Benfu and Liu, Miao and Bai, Changsen and Ruan, Yuxia and Wang, Yuanyuan and Qiu, Li and Hong, Yang and Wang, Xin and Li, Lifang and Li, Binghui

Journal:

Molecular therapy : the journal of the American Society of Gene Therapy (2020)

Expression levels of EPHB4, EFNB2 and caspase-8 are associated with clinicopathological features and progression of esophageal squamous cell cancer.

Authors:

Ni, Qianzhi and Chen, Pingping and Zhu, Bing and Li, Jingjing and Xie, Dong and Ma, Xingyuan

Journal:

Oncology letters (2020): 917-929

Dissecting DISC regulation via pharmacological targeting of caspase-8/c-FLIPL heterodimer.

Authors:

Hillert, Laura K and Ivanisenko, Nikita V and Busse, Denise and Espe, Johannes and König, Corinna and Peltek, Sergey E and Kolchanov, Nikolai A and Ivanisenko, Vladimir A and Lavrik, Inna N

Journal:

Cell death and differentiation (2020)

Edwardsiella piscicida type III protein EseJ suppresses apoptosis through down regulating type 1 fimbriae, which stimulate the cleavage of caspase-8.

Authors:

He, Tian Tian and Zhou, Ying and Liu, Ying Li and Li, Duan You and Nie, Pin and Li, Ai Hua and Xie, Hai Xia

Journal:

Cellular microbiology (2020): e13193

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
UNSPSC	12171501

Instrument settings

Flow cytometer
Excitation	640 nm laser
Emission	660/20 nm filter
Instrument specification(s)	APC channel

Fluorescence microscope
Excitation	Cy5 filter set
Emission	Cy5 filter set
Recommended plate	Black wall/clear bottom
Instrument specification(s)	Black wall/clear bottom

Fluorescence microplate reader
Excitation	640 nm
Emission	680 nm
Cutoff	665 nm
Recommended plate	Black wall/clear bottom
Instrument specification(s)	Bottom read mode

Documents

Protocol
Safety Data Sheet
Certificate of Analysis

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Page updated on November 2, 2025

Component A: iFluor® 647-LETD-FMK	1 vial
Component B: Washing Buffer	1 bottle (100 mL)
Component C: 500X Nuclear Green™ DCS1	1 vial (100 μL)
Component D: 500X Hoechst	1 vial (100 μL)