Cell Meter™ Live Cell Caspase 8 Binding Assay Kit Red Fluorescence

Flow cytometric analysis of active caspase 8 using Cell Meter™ Live Cells Caspase 8 detection kit in Jurkat cells. The cells were treated with 1 μM staurosporine for 5 hours (Green) while untreated cells were used as a control (Red). The staurosporine response was inhibited by Z-VAD-FMK (caspase inhibitor) shown as blue. Cells were incubated with iFluor 647-LETD-FMK for 1 hour at RT. The fluorescent intensity was measured using NovoCyte flow cytometer with 660/20 nm filter (APC channel).

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Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
Quotation	Request
International	See distributors
Shipping	Standard overnight for United States, inquire for international

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H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
UNSPSC	12171501

Alternative formats

Cell Meter™ Live Cell Caspase 8 Binding Assay Kit *Green Fluorescence*

Overview SDS Protocol

Our Cell Meter™ live cell caspases activity assay kits are based on fluorescent FMK inhibitors of caspases. These inhibitors are cell permeable and non-cytotoxic. Once inside the cell, the caspase inhibitors bind covalently to the active caspases. This Cell Meter™ Live Cell Caspase 8 Activity Assay Kit is designed to detect cell apoptosis by measuring caspase 8 activation in live cells. It is used for the quantification of activated caspase 8 activities in apoptotic cells, or for screening caspase 8 inhibitors. iFluor 647-LETD-FMK, the red label reagent, allows for direct detection of activated caspase 8 in apoptotic cells by fluorescence microscopy, flow cytometer, or fluorescent microplate reader. The kit provides all the essential components with an optimized assay protocol.

Platform

Flow cytometer

Excitation	640 nm laser
Emission	660/20 nm filter
Instrument specification(s)	APC channel

Fluorescence microscope

Excitation	Cy5 filter set
Emission	Cy5 filter set
Recommended plate	Black wall/clear bottom
Instrument specification(s)	Black wall/clear bottom

Fluorescence microplate reader

Excitation	640 nm
Emission	680 nm
Cutoff	665 nm
Recommended plate	Black wall/clear bottom
Instrument specification(s)	Bottom read mode

Components

Product Family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Correction Factor (260 nm)	Correction Factor (280 nm)
Cell Meter™ Live Cell Caspase 8 Binding Assay Kit Green Fluorescence	493	517	83000	0.32	0.178

Images

Figure 1. Flow cytometric analysis of active caspase 8 using Cell Meter™ Live Cells Caspase 8 detection kit in Jurkat cells. The cells were treated with 1 μM staurosporine for 5 hours (Green) while untreated cells were used as a control (Red). The staurosporine response was inhibited by Z-VAD-FMK (caspase inhibitor) shown as blue. Cells were incubated with iFluor 647-LETD-FMK for 1 hour at RT. The fluorescent intensity was measured using NovoCyte flow cytometer with 660/20 nm filter (APC channel).

References

View all 50 references: Citation Explorer

Cigarette smoke inhibits the NLRP3 inflammasome and leads to caspase-1 activation via the TLR4-TRIF-caspase-8 axis in human macrophages.
Authors: Buscetta, Marco and Di Vincenzo, Serena and Miele, Monica and Badami, Ester and Pace, Elisabetta and Cipollina, Chiara
Journal: FASEB journal : official publication of the Federation of American Societies for Experimental Biology (2020): 1819-1832

The nonreceptor tyrosine kinase SYK drives caspase-8/NLRP3 inflammasome-mediated autoinflammatory osteomyelitis.
Authors: Dasari, Tejasvi K and Geiger, Rechel and Karki, Rajendra and Banoth, Balaji and Sharma, Bhesh Raj and Gurung, Prajwal and Burton, Amanda and Kanneganti, Thirumala-Devi
Journal: The Journal of biological chemistry (2020): 3394-3400

New insights into the regulation of apoptosis, necroptosis, and pyroptosis by receptor interacting protein kinase 1 and caspase-8.
Authors: Schwarzer, Robin and Laurien, Lucie and Pasparakis, Manolis
Journal: Current opinion in cell biology (2020): 186-193

Caspase-8: The double-edged sword.
Authors: Mandal, Ranadip and Barrón, Joan Compte and Kostova, Izabela and Becker, Sven and Strebhardt, Klaus
Journal: Biochimica et biophysica acta. Reviews on cancer (2020): 188357

Triggering apoptosis by oroxylin A through caspase-8 activation and p62/SQSTM1 proteolysis.
Authors: Zhao, Yue and Zhu, Qin and Bu, Xiumin and Zhou, Yihui and Bai, Dongsheng and Guo, Qinglong and Gao, Yuan and Lu, Na
Journal: Redox biology (2020): 101392

Cancer Cells Employ Nuclear Caspase-8 to Overcome the p53-Dependent G2/M Checkpoint through Cleavage of USP28.
Authors: Müller, Ines and Strozyk, Elwira and Schindler, Sebastian and Beissert, Stefan and Oo, Htoo Zarni and Sauter, Thomas and Lucarelli, Philippe and Raeth, Sebastian and Hausser, Angelika and Al Nakouzi, Nader and Fazli, Ladan and Gleave, Martin E and Liu, He and Simon, Hans-Uwe and Walczak, Henning and Green, Douglas R and Bartek, Jiri and Daugaard, Mads and Kulms, Dagmar
Journal: Molecular cell (2020): 970-984.e7

Viral FLIP blocks Caspase-8 driven apoptosis in the gut in vivo.
Authors: Ruder, Barbara and Günther, Claudia and Stürzl, Michael and Neurath, Markus Friedrich and Cesarman, Ethel and Ballon, Gianna and Becker, Christoph
Journal: PloS one (2020): e0228441

A matter of life and death for caspase 8.
Authors: Willson, Joseph
Journal: Nature reviews. Molecular cell biology (2020): 63

Pharmacological Inhibition of Caspase-8 Suppresses Inflammation-Induced Angiogenesis in the Cornea.
Authors: Tian, Yunzhe and Li, He and Liu, Xiuxing and Xie, Lihui and Huang, Zhaohao and Li, Weihua and Li, Zhuang and Pan, Yuan and Chen, Xiaoqing and Su, Wenru
Journal: Biomolecules (2020)

High-Concentrate Feeding to Dairy Cows Induces Apoptosis via the NOD1/Caspase-8 Pathway in Mammary Epithelial Cells.
Authors: Ul Aabdin, Zain and Cheng, Xiaoye and Dai, Hongyu and Wang, Yan and Sahito, Benazir and Roy, Animesh Chandra and Memon, Meena Arif and Shen, Xiangzhen
Journal: Genes (2020)