Cell Meter™ Live Cell Caspase 8 Binding Assay Kit Red Fluorescence

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Additional ordering information

Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Shipping	Standard overnight for United States, inquire for international

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
UNSPSC	12171501

Alternative formats

Cell Meter™ Live Cell Caspase 8 Binding Assay Kit *Green Fluorescence*

Overview SDS Protocol

Platform

Flow cytometer

Excitation	640 nm laser
Emission	660/20 nm filter
Instrument specification(s)	APC channel

Fluorescence microscope

Excitation	Cy5 filter set
Emission	Cy5 filter set
Recommended plate	Black wall/clear bottom
Instrument specification(s)	Black wall/clear bottom

Fluorescence microplate reader

Excitation	640 nm
Emission	680 nm
Cutoff	665 nm
Recommended plate	Black wall/clear bottom
Instrument specification(s)	Bottom read mode

Components

Product Family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Correction Factor (260 nm)	Correction Factor (280 nm)
Cell Meter™ Live Cell Caspase 8 Binding Assay Kit Green Fluorescence	493	517	83000	0.32	0.178

Images

Figure 1. Flow cytometric analysis of active caspase 8 using Cell Meter™ Live Cells Caspase 8 detection kit in Jurkat cells. The cells were treated with 1 μM staurosporine for 5 hours (Green) while untreated cells were used as a control (Red). The staurosporine response was inhibited by Z-VAD-FMK (caspase inhibitor) shown as blue. Cells were incubated with iFluor 647-LETD-FMK for 1 hour at RT. The fluorescent intensity was measured using NovoCyte flow cytometer with 660/20 nm filter (APC channel).

References

View all 50 references: Citation Explorer

Cigarette smoke inhibits the NLRP3 inflammasome and leads to caspase-1 activation via the TLR4-TRIF-caspase-8 axis in human macrophages.
Authors: Buscetta, Marco and Di Vincenzo, Serena and Miele, Monica and Badami, Ester and Pace, Elisabetta and Cipollina, Chiara
Journal: FASEB journal : official publication of the Federation of American Societies for Experimental Biology (2020): 1819-1832

Caspase-8 Induces Lysosome-Associated Cell Death in Cancer Cells.
Authors: Zhong, Benfu and Liu, Miao and Bai, Changsen and Ruan, Yuxia and Wang, Yuanyuan and Qiu, Li and Hong, Yang and Wang, Xin and Li, Lifang and Li, Binghui
Journal: Molecular therapy : the journal of the American Society of Gene Therapy (2020)

Expression levels of EPHB4, EFNB2 and caspase-8 are associated with clinicopathological features and progression of esophageal squamous cell cancer.
Authors: Ni, Qianzhi and Chen, Pingping and Zhu, Bing and Li, Jingjing and Xie, Dong and Ma, Xingyuan
Journal: Oncology letters (2020): 917-929

Dissecting DISC regulation via pharmacological targeting of caspase-8/c-FLIPL heterodimer.
Authors: Hillert, Laura K and Ivanisenko, Nikita V and Busse, Denise and Espe, Johannes and König, Corinna and Peltek, Sergey E and Kolchanov, Nikolai A and Ivanisenko, Vladimir A and Lavrik, Inna N
Journal: Cell death and differentiation (2020)

Edwardsiella piscicida type III protein EseJ suppresses apoptosis through down regulating type 1 fimbriae, which stimulate the cleavage of caspase-8.
Authors: He, Tian Tian and Zhou, Ying and Liu, Ying Li and Li, Duan You and Nie, Pin and Li, Ai Hua and Xie, Hai Xia
Journal: Cellular microbiology (2020): e13193

Target inhibition of caspase-8 alleviates brain damage after subarachnoid hemorrhage.
Authors: Ke, Da-Qiang and Chen, Zhi-Yang and Li, Zhou-Ling and Huang, Xia and Liang, Hui
Journal: Neural regeneration research (2020): 1283-1289

Luteolin-regulated MicroRNA-301-3p Targets Caspase-8 and Modulates TRAIL Sensitivity in PANC-1 Cells.
Authors: Moeng, Sokviseth and Son, Seung Wan and Seo, Hyun Ah and Lee, Jong Sun and Kim, Chung Kwon and Kuh, Hyo Jeong and Park, Jong Kook
Journal: Anticancer research (2020): 723-731

Ptpn6 inhibits caspase-8- and Ripk3/Mlkl-dependent inflammation.
Authors: Speir, Mary and Nowell, Cameron J and Chen, Alyce A and O'Donnell, Joanne A and Shamie, Isaac S and Lakin, Paul R and D'Cruz, Akshay A and Braun, Roman O and Babon, Jeff J and Lewis, Rowena S and Bliss-Moreau, Meghan and Shlomovitz, Inbar and Wang, Shu and Cengia, Louise H and Stoica, Anca I and Hakem, Razq and Kelliher, Michelle A and O'Reilly, Lorraine A and Patsiouras, Heather and Lawlor, Kate E and Weller, Edie and Lewis, Nathan E and Roberts, Andrew W and Gerlic, Motti and Croker, Ben A
Journal: Nature immunology (2020): 54-64

Chrm3 protects against acinar cell necrosis by stabilizing caspase-8 expression in severe acute pancreatitis mice model.
Authors: Huang, Ning and Murtaza, Ghulam and Wang, Lujing and Luan, Jing and Wang, Xinlei and Sun, Yumiao and Wu, Xing and Tao, Yuxi and Shi, Shuoxi and Cao, Peihua and Qiao, Yu and Han, Dong and Kou, Jiayuan and Ma, Ning and Gao, Xu
Journal: Journal of cellular biochemistry (2020): 2618-2631

Yansuanmalingua inhibits replication of type 2 porcine reproductive and respiratory syndrome virus via activating the caspase-8 apoptosis pathway.
Authors: Ding, Yaozhong and Li, Guoxiu and Cheng, Fangzhen and Dai, Junfei and Hu, Yonghao and Zhang, Jie
Journal: Journal of basic microbiology (2020)