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Cell Meter™ Phosphatidylserine Apoptosis Assay Kit
Red Fluorescence Optimized for Microplate Readers
Our Cell Meter™ assay kits are a set of tools for monitoring cell viability. There are a variety of parameters that can be used for monitoring cell viability. This particular kit is designed to monitor cell apoptosis through measuring the translocation of phosphatidylserine (PS). In apoptosis, PS is transferred to the outer leaflet of the plasma membrane. The appearance of phosphatidylserine on the cell surface is a universal indicator of the initial/intermediate stages of cell apoptosis and can be detected before morphological changes can be observed. This kit uses a fluorescent sensor that specifically binds PS. This kit uses our proprietary fluorescent small molecule-based Apopxin™ PS sensor that specifically binds PS with affinity much higher than Annexin V (Kd < 10 nM). It has red fluorescence upon binding to membrane PS. It can be used in the formats of microplate, microscope and flow cytometer while most of other commercial apoptosis assay kits are only used with either microscope or flow cytometry platform.
Fluorescence Images of Jurkat cells in a Costar black wall/clear bottom 96-well plate stained with the Cell Meter&trade; Phosphatidylserine Apoptosis Assay Kit. Jurkat cells were treated without (Left)&nbsp;or with 20 &mu;M camptothecin (Right) for 5 hours. The fluorescence intensity was measured using a fluorescence microscope with Texas Red&reg; channel.
Fluorescence Images of Jurkat cells in a Costar black wall/clear bottom 96-well plate stained with the Cell Meter&trade; Phosphatidylserine Apoptosis Assay Kit. Jurkat cells were treated without (Left)&nbsp;or with 20 &mu;M camptothecin (Right) for 5 hours. The fluorescence intensity was measured using a fluorescence microscope with Texas Red&reg; channel.
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Price
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22792100 Tests
Price
 
Storage, safety and handling

Certificate of OriginDownload PDF
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12352200
Instrument settings

Fluorescence microplate reader
Excitation590 nm
Emission630 nm
Cutoff610 nm
Recommended plateBlack wall/clear bottom
Instrument specification(s)Bottom read mode
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Page updated on October 13, 2025