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Concanavalin A, XFD488 Labeled *XFD488 Same Structure to Alexa Fluor™ 488*

Live HeLa cells were stained with the fluorescent lectin Concanavalin A, XFD488 Labeled *XFD488 Same Structure to Alexa Fluor® 488* (Cat No. 25570) for 30 minutes at 37°C in HH buffer. The image was acquired using the FITC filter set.
Live HeLa cells were stained with the fluorescent lectin Concanavalin A, XFD488 Labeled *XFD488 Same Structure to Alexa Fluor® 488* (Cat No. 25570) for 30 minutes at 37°C in HH buffer. The image was acquired using the FITC filter set.
Live HeLa cells were stained with the fluorescent lectin Concanavalin A, XFD488 Labeled *XFD488 Same Structure to Alexa Fluor® 488* (Cat No. 25570) for 30 minutes at 37°C in HH buffer. The image was acquired using the FITC filter set.
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Physical properties
Molecular weightN/A
SolventWater
Spectral properties
Correction Factor (260 nm)0.3
Correction Factor (280 nm)0.11
Extinction coefficient (cm -1 M -1)73000
Excitation (nm)499
Emission (nm)520
Quantum yield0.921
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501

OverviewpdfSDSpdfProtocol


Molecular weight
N/A
Correction Factor (260 nm)
0.3
Correction Factor (280 nm)
0.11
Extinction coefficient (cm -1 M -1)
73000
Excitation (nm)
499
Emission (nm)
520
Quantum yield
0.921
XFD488 is manufactured by AAT Bioquest, and it has the same chemical structure of Alexa Fluor® 488 (Alexa Fluor® is the trademark of ThermoFisher). Concanavalin A (ConA) is a lectin that binds specifically to certain structures found in various sugars, glycoproteins and glycolipids. ConA is widely used in biology and biochemistry to characterize glycoproteins and other sugar-containing entities on the surface of various cells. It is also used to purify glycosylated macromolecules in lectin affinity chromatography, as well as to study immune regulation by various immune cells. ConA binds specifically α-D-mannosyl and α-D-glucosyl residues (two hexoses differing only in the alcohol on carbon 2) in terminal position of ramified structures from B-Glycans. It has 4 binding sites, corresponding to the 4 subunits. Concanavalin A (Con A) is one of the most widely used lectins in cell biology. XFD488-labeled Concanavalin A (equivalent to Alexa Fluor® 488 conjugate of Con A, Alexa Fluor® is the trademark of ThermoFisher) exhibits the bright, green fluorescence (Ex/Em maxima ~495/519 nm). XFD488 labeled Con A selectively binds to a-mannopyranosyl and a-glucopyranosyl residues.

Platform


Fluorescence microscope

ExcitationFITC filter set
EmissionFITC filter set
Recommended plateBlack wall/clear bottom

Spectrum


Open in Advanced Spectrum Viewer
spectrum

Spectral properties

Correction Factor (260 nm)0.3
Correction Factor (280 nm)0.11
Extinction coefficient (cm -1 M -1)73000
Excitation (nm)499
Emission (nm)520
Quantum yield0.921

Product Family


NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (260 nm)Correction Factor (280 nm)
Concanavalin A, XFD594 Labeled *XFD594 Same Structure to Alexa Fluor™ 594*590618920000.6610.430.56

Images


References


View all 50 references: Citation Explorer
Short-term high-fat diet intake leads to exacerbation of concanavalin A-induced liver injury through the induction of procoagulation state.
Authors: Nanizawa, Eri and Tamaki, Yuki and Sono, Reika and Miyashita, Rintaro and Hayashi, Yumi and Kanbe, Ayumu and Ito, Hiroyasu and Ishikawa, Tetsuya
Journal: Biochemistry and biophysics reports (2020): 100736
Maresin 1 mitigates concanavalin A-induced acute liver injury in mice by inhibiting ROS-mediated activation of NF-κB signaling.
Authors: Zhang, Peng and Yin, Yuping and Wang, Tao and Li, Wei and Li, Chengguo and Zeng, Xiangyu and Yang, Wenchang and Zhang, Ruizhi and Tang, Yu and Shi, Liang and Li, Ruidong and Tao, Kaixiong
Journal: Free radical biology & medicine (2020): 23-36
Membrane-associated gamma-glutamyl transferase and alkaline phosphatase in the context of concanavalin A- and wheat germ agglutinin-reactive glycans mark seminal prostasome populations from normozoospermic and oligozoospermic men.
Authors: Janković, Tamara and Goč, Sanja and Mitić, Ninoslav and Danilović Luković, Jelena and Janković, Miroslava
Journal: Upsala journal of medical sciences (2020): 10-18
Antioxidant Capacity and Hepatoprotective Role of Chitosan-Stabilized Selenium Nanoparticles in Concanavalin A-Induced Liver Injury in Mice.
Authors: Bai, Kaikai and Hong, Bihong and He, Jianlin and Huang, Wenwen
Journal: Nutrients (2020)
The farnesyltransferase inhibitor tipifarnib protects against autoimmune hepatitis induced by Concanavalin A.
Authors: Guo, Jie and Shirozu, Kazuhiro and Akahoshi, Tomohiko and Mizuta, Yukie and Murata, Masaharu and Yamaura, Ken
Journal: International immunopharmacology (2020): 106462
Concanavalin A-Rose Bengal bioconjugate for targeted Gram-negative antimicrobial photodynamic therapy.
Authors: Cantelli, Andrea and Piro, Francesca and Pecchini, Pietro and Di Giosia, Matteo and Danielli, Alberto and Calvaresi, Matteo
Journal: Journal of photochemistry and photobiology. B, Biology (2020): 111852
Developmental exposure to low doses of dichlorodiphenyltrichloroethane impairs proliferative response of thymic lymphocytes to Concanavalin A in rats.
Authors: Yaglova, Nataliya V and Tsomartova, Elina S and Obernikhin, Sergey S and Ivanova, Marina Y and Chereshneva, Elizaveta V and Muhamedova, Svetlana G and Lomanovskaya, Tatiana A and Yaglov, Valentin V
Journal: Heliyon (2020): e03608
Concanavalin A Toxicity Towards Potato Psyllid and Apoptosis Induction in Midgut Cells.
Authors: Tang, Xiao-Tian and Ibanez, Freddy and Tamborindeguy, Cecilia
Journal: Insects (2020)
Concanavalin A Enhanced Proliferation and Osteogenic Differentiation of Dental Pulp Stem Cells.
Authors: Suardita, Ketut and Arundina, Ira and Tedjosasongko, Udijanto and Yuliati, Anita and Peeters, Harry Huiz and Wijaksana, I Komang Evan and Surboyo, Meircurius Dwi Condro
Journal: European journal of dentistry (2020): 123-127
Mycophenolate mofetil attenuates concanavalin A-induced acute liver injury through modulation of TLR4/NF-κB and Nrf2/HO-1 pathways.
Authors: Serrya, Marwa S and Zaghloul, Marwa S
Journal: Pharmacological reports : PR (2020)