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Cy3®-streptavidin conjugate
Streptavidin conjugates are widely used together with a conjugate of biotin for specific detection of a variety of proteins, protein motifs, nucleic acids and other molecules since streptavidin has a very high binding affinity for biotin. This Cy3-streptavidin conjugate comprises streptavidin (as the biotin-binding protein) with Cy3 covalently attached (as the fluorescent label). Our Cy3-streptavidin conjugate was prepared using AAT Bioquest's proprietary labeling technology. It demonstrated much brighter signal compared to the similar Cy3-streptavidin conjugates from other commercial sources, thus can significantly increase assay sensitivities. It is commonly used as a second step reagent for indirect immunofluorescent staining, when used in conjunction with biotinylated primary antibodies. It is a valuable tool for biotin-streptavidin-based biological assays and tests using flow cytometry with a Cy3 filter set. A variety of the complementary biotinylated reagents are available from numerous commercial vendors.
HeLa cells were incubated with mouse anti-tubulin and biotin goat anti-mouse IgG followed by AAT's Cy3®-streptavidin conjugate (Red). Cell nuclei were stained with Hoechst 33342 (Blue, Cat#17530).
HeLa cells were incubated with mouse anti-tubulin and biotin goat anti-mouse IgG followed by AAT's Cy3®-streptavidin conjugate (Red). Cell nuclei were stained with Hoechst 33342 (Blue, Cat#17530).
protocol
Protocol
sds
SDS
CatalogSize
Price
Quantity
169121 mg
Price
 
Physical properties
Molecular weight~52000
SolventWater
Spectral properties
Correction factor (260 nm)0.07
Correction factor (280 nm)0.073
Extinction coefficient (cm -1 M -1)
150000
1
Excitation (nm)555
Emission (nm)569
Quantum yield
0.15
1
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12352200
Documents
Protocol
Safety Data Sheet
Certificate of Origin
Certificate of Analysis
Brochure: Secondary Detection Probes & Kits
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Page updated on October 29, 2025