Cyanine-3- dUTP [Cy3-dUTP] *1 mM in TE Buffer (pH 7.5)*
The dye-modified deoxyuridine 5'-triphosphates (such as aminoallyl-dUTP) can be used to produce dye-containing DNA by conventional enzymatic incorporation methods such as reverse transcription, nick translation, random primed labeling, or PCR. This enzymatic fluorescence labeling method is widely used for both FISH probes and microarray-based experiments. This Cy3-dUTP conjugate can be used as a red fluorescence color with Spectrum Orange™ filter set (Spectrum Orange™ is the trademark of Vysis). It is functionally equivalent to Cyanine 3-dUTP (Perkin Elmer’s NEL578001EA).
Spectrum
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Product family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
Cyanine 3 bisacid [equivalent to Cy3® bisacid] | 555 | 569 | 1500001 | 0.151 | 0.07 | 0.073 |
Cyanine 3 monoacid [equivalent to Cy3® acid] | 555 | 569 | 1500001 | 0.151 | 0.07 | 0.073 |
Cyanine 3 maleimide [equivalent to Cy3® maleimide] | 555 | 569 | 1500001 | 0.151 | 0.07 | 0.073 |
Cyanine 3 azide [equivalent to Cy3® azide] | 555 | 569 | 1500001 | 0.151 | 0.07 | 0.073 |
Cyanine 3 alkyne [equivalent to Cy3® alkyne] | 555 | 569 | 1500001 | 0.151 | 0.07 | 0.073 |
Cyanine 3 amine [equivalent to Cy3® amine] | 555 | 569 | 1500001 | 0.151 | 0.07 | 0.073 |
Cyanine 3 hydrazide [equivalent to Cy3® hydrazide] | 555 | 569 | 1500001 | 0.151 | 0.07 | 0.073 |
Cyanine-5- dUTP [Cy5-dUTP] *1 mM in TE Buffer (pH 7.5)* | 651 | 670 | 2500001 | 0.271, 0.42 | 0.02 | 0.03 |
References
View all 48 references: Citation Explorer
A Fluorescent DNA Hydrogel Aptasensor Based on the Self-Assembly of Rolling Circle Amplification Products for Sensitive Detection of Ochratoxin A.
Authors: Hao, Liling and Wang, Wei and Shen, Xueqing and Wang, Shuliu and Li, Qian and An, Faliang and Wu, Shijia
Journal: Journal of agricultural and food chemistry (2020): 369-375
Authors: Hao, Liling and Wang, Wei and Shen, Xueqing and Wang, Shuliu and Li, Qian and An, Faliang and Wu, Shijia
Journal: Journal of agricultural and food chemistry (2020): 369-375
Engineering nicking enzymes that preferentially nick 5-methylcytosine-modified DNA.
Authors: Gutjahr, Alice and Xu, Shuang-yong
Journal: Nucleic acids research (2014): e77
Authors: Gutjahr, Alice and Xu, Shuang-yong
Journal: Nucleic acids research (2014): e77
Digital detection of multiple minority mutants in stool DNA for noninvasive colorectal cancer diagnosis.
Authors: Deng, Lili and Qi, Zongtai and Zou, Binjie and Wu, Haiping and Huang, Huan and Kajiyama, Tomoharu and Kambara, Hideki and Zhou, Guohua
Journal: Analytical chemistry (2012): 5645-52
Authors: Deng, Lili and Qi, Zongtai and Zou, Binjie and Wu, Haiping and Huang, Huan and Kajiyama, Tomoharu and Kambara, Hideki and Zhou, Guohua
Journal: Analytical chemistry (2012): 5645-52
Digital analysis of the expression levels of multiple colorectal cancer-related genes by multiplexed digital-PCR coupled with hydrogel bead-array.
Authors: Qi, Zongtai and Ma, Yinjiao and Deng, Lili and Wu, Haiping and Zhou, Guohua and Kajiyama, Tomoharu and Kambara, Hideki
Journal: The Analyst (2011): 2252-9
Authors: Qi, Zongtai and Ma, Yinjiao and Deng, Lili and Wu, Haiping and Zhou, Guohua and Kajiyama, Tomoharu and Kambara, Hideki
Journal: The Analyst (2011): 2252-9
Microisolation and microcloning of bovine X-chromosomes for identification of sorted buffalo (Bubalus bubalis) spermatozoa.
Authors: Zhuang, Xin-jie and Lu, Yang-qing and Zhang, Ming and Lu, Sheng-sheng and Lu, Ke-huan
Journal: Animal reproduction science (2011): 32-6
Authors: Zhuang, Xin-jie and Lu, Yang-qing and Zhang, Ming and Lu, Sheng-sheng and Lu, Ke-huan
Journal: Animal reproduction science (2011): 32-6
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