Cyanine-3- dUTP [Cy3-dUTP] 1 mM in TE Buffer (pH 7.5)

The dye-modified deoxyuridine 5'-triphosphates (such as aminoallyl-dUTP) can be used to produce dye-containing DNA by conventional enzymatic incorporation methods such as reverse transcription, nick translation, random primed labeling, or PCR. This enzymatic fluorescence labeling method is widely used for both FISH probes and microarray-based experiments. This Cy3-dUTP conjugate can be used as a red fluorescence color with Spectrum Orange™ filter set (Spectrum Orange™ is the trademark of Vysis). It is functionally equivalent to Cyanine 3-dUTP (Perkin Elmer’s NEL578001EA).

Spectrum

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Product family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Quantum yield	Correction Factor (260 nm)	Correction Factor (280 nm)
Cyanine 3 bisacid [equivalent to Cy3® bisacid]	555	569	150000¹	0.15¹	0.07	0.073
Cyanine 3 monoacid [equivalent to Cy3® acid]	555	569	150000¹	0.15¹	0.07	0.073
Cyanine 3 maleimide [equivalent to Cy3® maleimide]	555	569	150000¹	0.15¹	0.07	0.073
Cyanine 3 azide [equivalent to Cy3® azide]	555	569	150000¹	0.15¹	0.07	0.073
Cyanine 3 alkyne [equivalent to Cy3® alkyne]	555	569	150000¹	0.15¹	0.07	0.073
Cyanine 3 amine [equivalent to Cy3® amine]	555	569	150000¹	0.15¹	0.07	0.073
Cyanine 3 hydrazide [equivalent to Cy3® hydrazide]	555	569	150000¹	0.15¹	0.07	0.073
Cyanine-5- dUTP [Cy5-dUTP] 1 mM in TE Buffer (pH 7.5)	651	670	250000¹	0.27¹, 0.4²	0.02	0.03

References

View all 48 references: Citation Explorer

A Fluorescent DNA Hydrogel Aptasensor Based on the Self-Assembly of Rolling Circle Amplification Products for Sensitive Detection of Ochratoxin A.
Authors: Hao, Liling and Wang, Wei and Shen, Xueqing and Wang, Shuliu and Li, Qian and An, Faliang and Wu, Shijia
Journal: Journal of agricultural and food chemistry (2020): 369-375

Engineering nicking enzymes that preferentially nick 5-methylcytosine-modified DNA.
Authors: Gutjahr, Alice and Xu, Shuang-yong
Journal: Nucleic acids research (2014): e77

Digital detection of multiple minority mutants in stool DNA for noninvasive colorectal cancer diagnosis.
Authors: Deng, Lili and Qi, Zongtai and Zou, Binjie and Wu, Haiping and Huang, Huan and Kajiyama, Tomoharu and Kambara, Hideki and Zhou, Guohua
Journal: Analytical chemistry (2012): 5645-52

Digital analysis of the expression levels of multiple colorectal cancer-related genes by multiplexed digital-PCR coupled with hydrogel bead-array.
Authors: Qi, Zongtai and Ma, Yinjiao and Deng, Lili and Wu, Haiping and Zhou, Guohua and Kajiyama, Tomoharu and Kambara, Hideki
Journal: The Analyst (2011): 2252-9

Microisolation and microcloning of bovine X-chromosomes for identification of sorted buffalo (Bubalus bubalis) spermatozoa.
Authors: Zhuang, Xin-jie and Lu, Yang-qing and Zhang, Ming and Lu, Sheng-sheng and Lu, Ke-huan
Journal: Animal reproduction science (2011): 32-6

Page updated on October 24, 2024

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Physical properties

Molecular weight	1150.00
Solvent	Water

Spectral properties

Correction Factor (260 nm)	0.07
Correction Factor (280 nm)	0.073
Extinction coefficient (cm ^-1 M ^-1)	150000¹
Excitation (nm)	555
Emission (nm)	569
Quantum yield	0.15¹

Storage, safety and handling

Certificate of Origin	Download PDF
H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501