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Cyanine-3- dUTP [Cy3-dUTP] *1 mM in TE Buffer (pH 7.5)*

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Fluorescence In Situ Hybridization of Cy3 and iFluor® 555-dUTP labelled Telomere probes in metaphase HeLa cells.
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Physical properties
Molecular weight1150.00
Spectral properties
Correction Factor (260 nm)0.07
Correction Factor (280 nm)0.073
Extinction coefficient (cm -1 M -1)1500001
Excitation (nm)555
Emission (nm)569
Quantum yield0.151
Storage, safety and handling
Certificate of OriginDownload PDF
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure


Molecular weight
Correction Factor (260 nm)
Correction Factor (280 nm)
Extinction coefficient (cm -1 M -1)
Excitation (nm)
Emission (nm)
Quantum yield
The dye-modified deoxyuridine 5'-triphosphates (such as aminoallyl-dUTP) can be used to produce dye-containing DNA by conventional enzymatic incorporation methods such as reverse transcription, nick translation, random primed labeling, or PCR. This enzymatic fluorescence labeling method is widely used for both FISH probes and microarray-based experiments. This Cy3-dUTP conjugate can be used as a red fluorescence color with Spectrum Orange™ filter set (Spectrum Orange™ is the trademark of Vysis). It is functionally equivalent to Cyanine 3-dUTP (Perkin Elmer’s NEL578001EA).


Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of Cyanine-3- dUTP [Cy3-dUTP] *1 mM in TE Buffer (pH 7.5)* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM86.957 µL434.783 µL869.565 µL4.348 mL8.696 mL
5 mM17.391 µL86.957 µL173.913 µL869.565 µL1.739 mL
10 mM8.696 µL43.478 µL86.957 µL434.783 µL869.565 µL

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Spectral properties

Correction Factor (260 nm)0.07
Correction Factor (280 nm)0.073
Extinction coefficient (cm -1 M -1)1500001
Excitation (nm)555
Emission (nm)569
Quantum yield0.151

Product Family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (260 nm)Correction Factor (280 nm)
Cyanine 3 bisacid [equivalent to Cy3® bisacid]55556915000010.1510.070.073
Cyanine 3 monoacid [equivalent to Cy3® acid]55556915000010.1510.070.073
Cyanine 3 maleimide [equivalent to Cy3® maleimide]55556915000010.1510.070.073
Cyanine 3 azide [equivalent to Cy3® azide]55556915000010.1510.070.073
Cyanine 3 alkyne [equivalent to Cy3® alkyne]55556915000010.1510.070.073
Cyanine 3 amine [equivalent to Cy3® amine]55556915000010.1510.070.073
Cyanine 3 hydrazide [equivalent to Cy3® hydrazide]55556915000010.1510.070.073
Cyanine-5- dUTP [Cy5-dUTP] *1 mM in TE Buffer (pH 7.5)*65167025000010.271, 0.420.020.03



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