Cyanine-5- dUTP [Cy5-dUTP] *1 mM in TE Buffer (pH 7.5)*
The dye-modified deoxyuridine 5'-triphosphates (such as aminoallyl-dUTP) can be used to produce dye-containing DNA by conventional enzymatic incorporation methods such as reverse transcription, nick translation, random primed labeling, or PCR. This enzymatic fluorescence labeling method is widely used for both FISH probes and microarray-based experiments. This Cy5-dUTP conjugate can be used as a red fluorescence color with SpectrumFRed™ filter set (SpectrumFRed™ is the trademark of Vysis). It is functionally equivalent to Cyanine 5-dUTP (Perkin Elmer’s NEL579001EA).
Spectrum
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Product family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) | Correction Factor (482 nm) | Correction Factor (565 nm) |
Cyanine 5 monoacid [equivalent to Cy5® acid] | 651 | 670 | 2500001 | 0.271, 0.42 | 0.02 | 0.03 | 0.009 | 0.09 |
Cyanine 5 maleimide [equivalent to Cy5® maleimide] | 651 | 670 | 2500001 | 0.271, 0.42 | 0.02 | 0.03 | 0.009 | 0.09 |
Cyanine 5 azide [equivalent to Cy5® azide] | 651 | 670 | 2500001 | 0.271, 0.42 | 0.02 | 0.03 | 0.009 | 0.09 |
Cyanine 5 alkyne [equivalent to Cy5® alkyne] | 651 | 670 | 2500001 | 0.271, 0.42 | 0.02 | 0.03 | 0.009 | 0.09 |
Cyanine 5 amine [equivalent to Cy5® amine] | 651 | 670 | 2500001 | 0.271, 0.42 | 0.02 | 0.03 | 0.009 | 0.09 |
Cyanine 5 hydrazide [equivalent to Cy5® hydrazide] | 651 | 670 | 2500001 | 0.271, 0.42 | 0.02 | 0.03 | 0.009 | 0.09 |
Cyanine 5 bisacid [equivalent to Cy5® bisacid] | 651 | 670 | 2500001 | 0.271, 0.42 | 0.02 | 0.03 | 0.009 | 0.09 |
Cyanine-3- dUTP [Cy3-dUTP] *1 mM in TE Buffer (pH 7.5)* | 555 | 569 | 1500001 | 0.151 | 0.07 | 0.073 | - | - |
Cyanine 5-dATP [Cy5-dATP] | 651 | 670 | 2500001 | 0.271, 0.42 | 0.02 | 0.03 | 0.009 | 0.09 |
Citations
View all 1 citations: Citation Explorer
Probe-based qPCR: Probe Compatibility and Multiplexing with Luna{\textregistered} Universal Probe qPCR Master Mix
Authors: Tanner, Nathan A and Gray, Andrew N and Nichols, Nicole M
Journal: Probe : 17
Authors: Tanner, Nathan A and Gray, Andrew N and Nichols, Nicole M
Journal: Probe : 17
References
View all 38 references: Citation Explorer
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Journal: BMC cancer (2018): 772
dUTPs conjugated with zwitterionic Cy3 or Cy5 fluorophore analogues are effective substrates for DNA amplification and labelling by Taq polymerase.
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