Cyanine-5- dUTP [Cy5-dUTP] 1 mM in TE Buffer (pH 7.5)

Name: Cyanine-5- dUTP [Cy5-dUTP] *1 mM in TE Buffer (pH 7.5)*
Brand: AAT Bioquest
SKU: 17026
Availability: InStock

Documents

Safety data sheet

Product protocol

Certificate of analysis

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FAQ

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Application notes

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The dye-modified deoxyuridine 5'-triphosphates (such as aminoallyl-dUTP) can be used to produce dye-containing DNA by conventional enzymatic incorporation methods such as reverse transcription, nick translation, random primed labeling, or PCR. This enzymatic fluorescence labeling method is widely used for both FISH probes and microarray-based experiments. This Cy5-dUTP conjugate can be used as a red fluorescence color with SpectrumFRed™ filter set (SpectrumFRed™ is the trademark of Vysis). It is functionally equivalent to Cyanine 5-dUTP (Perkin Elmer’s NEL579001EA).

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Physical properties

Molecular weight	1162.01
Solvent	Water

Spectral properties

Correction Factor (260 nm)	0.02
Correction Factor (280 nm)	0.03
Correction Factor (482 nm)	0.009
Correction Factor (565 nm)	0.09
Extinction coefficient (cm ^-1 M ^-1)	250000¹
Excitation (nm)	651
Emission (nm)	670
Quantum yield	0.27¹, 0.4²

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Spectrum

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Product family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Quantum yield	Correction Factor (260 nm)	Correction Factor (280 nm)	Correction Factor (482 nm)	Correction Factor (565 nm)
Cyanine 5 monoacid [equivalent to Cy5® acid]	651	670	250000¹	0.27¹, 0.4²	0.02	0.03	0.009	0.09
Cyanine 5 maleimide [equivalent to Cy5® maleimide]	651	670	250000¹	0.27¹, 0.4²	0.02	0.03	0.009	0.09
Cyanine 5 azide [equivalent to Cy5® azide]	651	670	250000¹	0.27¹, 0.4²	0.02	0.03	0.009	0.09
Cyanine 5 alkyne [equivalent to Cy5® alkyne]	651	670	250000¹	0.27¹, 0.4²	0.02	0.03	0.009	0.09
Cyanine 5 amine [equivalent to Cy5® amine]	651	670	250000¹	0.27¹, 0.4²	0.02	0.03	0.009	0.09
Cyanine 5 hydrazide [equivalent to Cy5® hydrazide]	651	670	250000¹	0.27¹, 0.4²	0.02	0.03	0.009	0.09
Cyanine 5 bisacid [equivalent to Cy5® bisacid]	651	670	250000¹	0.27¹, 0.4²	0.02	0.03	0.009	0.09
Cyanine-3- dUTP [Cy3-dUTP] 1 mM in TE Buffer (pH 7.5)	555	569	150000¹	0.15¹	0.07	0.073	-	-
Cyanine 5-dATP [Cy5-dATP]	651	670	250000¹	0.27¹, 0.4²	0.02	0.03	0.009	0.09

Citations

View all 1 citations: Citation Explorer

Probe-based qPCR: Probe Compatibility and Multiplexing with Luna{\textregistered} Universal Probe qPCR Master Mix
Authors: Tanner, Nathan A and Gray, Andrew N and Nichols, Nicole M
Journal: Probe : 17

References

View all 38 references: Citation Explorer

Loss of PDPK1 abrogates resistance to gemcitabine in label-retaining pancreatic cancer cells.
Authors: Li, Dandan and Mullinax, John E and Aiken, Taylor and Xin, Hongwu and Wiegand, Gordon and Anderson, Andrew and Thorgeirsson, Snorri and Avital, Itzhak and Rudloff, Udo
Journal: BMC cancer (2018): 772

dUTPs conjugated with zwitterionic Cy3 or Cy5 fluorophore analogues are effective substrates for DNA amplification and labelling by Taq polymerase.
Authors: Zasedateleva, Olga A and Vasiliskov, Vadim A and Surzhikov, Sergey A and Kuznetsova, Viktoriya E and Shershov, Valeriy E and Guseinov, Timur O and Smirnov, Igor P and Yurasov, Roman A and Spitsyn, Maksim A and Chudinov, Alexander V
Journal: Nucleic acids research (2018): e73

Mass-spectrometry analysis of modifications at DNA termini induced by DNA polymerases.
Authors: Smirnov, Igor P and Kolganova, Natalia A and Vasiliskov, Vadim A and Chudinov, Alexander V and Timofeev, Edward N
Journal: Scientific reports (2017): 6674

Separation of replication and transcription domains in nucleoli.
Authors: Smirnov, E and Borkovec, J and Kováčik, L and Svidenská, S and Schröfel, A and Skalníková, M and Švindrych, Z and Křížek, P and Ovesný, M and Hagen, G M and Juda, P and Michalová, K and Cardoso, M C and Cmarko, D and Raška, I
Journal: Journal of structural biology (2014): 259-66

A prenatally ascertained, maternally inherited 14.8 Mb duplication of chromosomal bands Xq13.2-q21.31 associated with multiple congenital abnormalities in a male fetus.
Authors: Sismani, C and Donoghue, J and Alexandrou, A and Karkaletsi, M and Christopoulou, S and Konstantinidou, A E and Livanos, P and Patsalis, P C and Velissariou, V
Journal: Gene (2013): 138-42

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