Name: Cyanine-5- dUTP [Cy5-dUTP] *1 mM in TE Buffer (pH 7.5)*
Brand: AAT Bioquest
SKU: 17026
Availability: InStock

Cyanine-5- dUTP

Cy5-dUTP; 1 mM in TE Buffer (pH 7.5)

The dye-modified deoxyuridine 5'-triphosphates (such as aminoallyl-dUTP) can be used to produce dye-containing DNA by conventional enzymatic incorporation methods such as reverse transcription, nick translation, random primed labeling, or PCR. This enzymatic fluorescence labeling method is widely used for both FISH probes and microarray-based experiments. This Cy5-dUTP conjugate can be used as a red fluorescence color with SpectrumFRed™ filter set (SpectrumFRed™ is the trademark of Vysis). It is functionally equivalent to Cyanine 5-dUTP (Perkin Elmer’s NEL579001EA).

Telomeric DNA fluorescence in situ hybridization (FISH) on metaphase spreads from HeLa cells using telomere probes with Cy5-dUTP (Cat. #17026).

Protocol

SDS

Catalog	Size	Price
17026	25 nmoles	Price
17026-100NM	100 nmoles	Price
17026-1UM	1 umole	Price

References

View all 38 references: Citation Explorer

Loss of PDPK1 abrogates resistance to gemcitabine in label-retaining pancreatic cancer cells.

Authors:

Li, Dandan and Mullinax, John E and Aiken, Taylor and Xin, Hongwu and Wiegand, Gordon and Anderson, Andrew and Thorgeirsson, Snorri and Avital, Itzhak and Rudloff, Udo

Journal:

BMC cancer (2018): 772

dUTPs conjugated with zwitterionic Cy3 or Cy5 fluorophore analogues are effective substrates for DNA amplification and labelling by Taq polymerase.

Authors:

Zasedateleva, Olga A and Vasiliskov, Vadim A and Surzhikov, Sergey A and Kuznetsova, Viktoriya E and Shershov, Valeriy E and Guseinov, Timur O and Smirnov, Igor P and Yurasov, Roman A and Spitsyn, Maksim A and Chudinov, Alexander V

Journal:

Nucleic acids research (2018): e73

Mass-spectrometry analysis of modifications at DNA termini induced by DNA polymerases.

Authors:

Smirnov, Igor P and Kolganova, Natalia A and Vasiliskov, Vadim A and Chudinov, Alexander V and Timofeev, Edward N

Journal:

Scientific reports (2017): 6674

Separation of replication and transcription domains in nucleoli.

Authors:

Smirnov, E and Borkovec, J and Kováčik, L and Svidenská, S and Schröfel, A and Skalníková, M and Švindrych, Z and Křížek, P and Ovesný, M and Hagen, G M and Juda, P and Michalová, K and Cardoso, M C and Cmarko, D and Raška, I

Journal:

Journal of structural biology (2014): 259-66

A prenatally ascertained, maternally inherited 14.8 Mb duplication of chromosomal bands Xq13.2-q21.31 associated with multiple congenital abnormalities in a male fetus.

Authors:

Sismani, C and Donoghue, J and Alexandrou, A and Karkaletsi, M and Christopoulou, S and Konstantinidou, A E and Livanos, P and Patsalis, P C and Velissariou, V

Journal:

Gene (2013): 138-42

Physical properties

Molecular weight	1162.01
Solvent	Water

Spectral properties

Correction factor (260 nm)	0.02
Correction factor (280 nm)	0.03
Correction factor (482 nm)	0.009
Correction factor (565 nm)	0.09
Extinction coefficient (cm ^-1 M ^-1)	250000 ¹
Excitation (nm)	651
Emission (nm)	670
Quantum yield	0.27 ¹ , 0.4 ²

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure

Documents

Protocol
Safety Data Sheet
Certificate of Analysis
Flyer: Labeled dUTPs

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Page updated on December 4, 2025