Cyanine 5-dATP [Cy5-dATP]

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Physical properties

Molecular weight	1182.05
Solvent	Water

Spectral properties

Correction Factor (260 nm)	0.02
Correction Factor (280 nm)	0.03
Correction Factor (482 nm)	0.009
Correction Factor (565 nm)	0.09
Extinction coefficient (cm ^-1 M ^-1)	250000¹
Excitation (nm)	651
Emission (nm)	670
Quantum yield	0.27¹, 0.4²

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Overview SDS Protocol

Molecular weight

1182.05

Correction Factor (260 nm)

0.02

Correction Factor (280 nm)

0.03

Correction Factor (482 nm)

0.009

Correction Factor (565 nm)

0.09

Extinction coefficient (cm ^-1 M ^-1)

250000¹

Excitation (nm)

651

Emission (nm)

670

Quantum yield

0.27¹, 0.4²

The dye-modified deoxyadenosine 5'-triphosphates are widely used for a variety of non-radioactive DNA labeling reactions including nick translation, random prime labeling, cDNA labeling and 3’-end labeling. Cy5-dATP can be used to produce Cy5-containing DNA by conventional enzymatic incorporation methods such as reverse transcription, nick translation, random primed labeling, or PCR. This enzymatic fluorescence labeling method is widely used for both FISH probes and microarray-based experiments. This Cy5-dATP conjugate can be used as a red fluorescence color with SpectrumFRed™ filter set (SpectrumFRed™ is the trademark of Vysis). It is functionally equivalent to Cyanine 5-dATP (Perkin Elmer’s NEL593001EA) with significantly improved stability.

Calculators

Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of Cyanine 5-dATP [Cy5-dATP] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	84.599 µL	422.994 µL	845.988 µL	4.23 mL	8.46 mL
5 mM	16.92 µL	84.599 µL	169.198 µL	845.988 µL	1.692 mL
10 mM	8.46 µL	42.299 µL	84.599 µL	422.994 µL	845.988 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
	/		=		x		=

Spectrum

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Spectral properties

Correction Factor (260 nm)	0.02
Correction Factor (280 nm)	0.03
Correction Factor (482 nm)	0.009
Correction Factor (565 nm)	0.09
Extinction coefficient (cm ^-1 M ^-1)	250000¹
Excitation (nm)	651
Emission (nm)	670
Quantum yield	0.27¹, 0.4²

Product Family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Quantum yield	Correction Factor (260 nm)	Correction Factor (280 nm)	Correction Factor (482 nm)	Correction Factor (565 nm)
Cyanine 5 monoacid [equivalent to Cy5® acid]	651	670	250000¹	0.27¹, 0.4²	0.02	0.03	0.009	0.09
Cyanine 5 maleimide [equivalent to Cy5® maleimide]	651	670	250000¹	0.27¹, 0.4²	0.02	0.03	0.009	0.09
Cyanine 5 azide [equivalent to Cy5® azide]	651	670	250000¹	0.27¹, 0.4²	0.02	0.03	0.009	0.09
Cyanine 5 alkyne [equivalent to Cy5® alkyne]	651	670	250000¹	0.27¹, 0.4²	0.02	0.03	0.009	0.09
Cyanine 5 amine [equivalent to Cy5® amine]	651	670	250000¹	0.27¹, 0.4²	0.02	0.03	0.009	0.09
Cyanine 5 hydrazide [equivalent to Cy5® hydrazide]	651	670	250000¹	0.27¹, 0.4²	0.02	0.03	0.009	0.09
Cyanine 5 bisacid [equivalent to Cy5® bisacid]	651	670	250000¹	0.27¹, 0.4²	0.02	0.03	0.009	0.09
Cyanine-5- dUTP [Cy5-dUTP] 1 mM in Tris Buffer (pH 7.5)	651	670	250000¹	0.27¹, 0.4²	0.02	0.03	0.009	0.09

Images

Figure 1. Chemical structure for Cyanine 5-dATP [Cy5-dATP].

Citations

View all 1 citations: Citation Explorer

Probe-based qPCR: Probe Compatibility and Multiplexing with Luna{\textregistered} Universal Probe qPCR Master Mix
Authors: Tanner, Nathan A and Gray, Andrew N and Nichols, Nicole M
Journal: Probe : 17

References

View all 50 references: Citation Explorer

New bioluminescence pyrophosphate assay for high-sensitivity detection of food-borne pathogens.
Authors: Fei, Zhongjie and Zhou, Dongrui and Li, Na and Xiao, Pengfeng
Journal: Luminescence : the journal of biological and chemical luminescence (2020): 355-364

2-Formyl-dATP as Substrate for Polymerase Synthesis of Reactive DNA Bearing an Aldehyde Group in the Minor Groove.
Authors: Krömer, Matouš and Brunderová, Mária and Ivancová, Ivana and Poštová Slavětínská, Lenka and Hocek, Michal
Journal: ChemPlusChem (2020): 1164-1170

Fluorescent dATP for DNA Synthesis In Vivo.
Authors: Schreier, Verena N and Loehr, Morten O and Deng, Ting and Lattmann, Evelyn and Hajnal, Alex and Neuhauss, Stephan C F and Luedtke, Nathan W
Journal: ACS chemical biology (2020): 2996-3003

Terminal deoxynucleotidyl transferase-initiated molecule beacons arrayed aptamer probe for sensitive detection of metastatic colorectal cancer cells.
Authors: Zhao, Yujie and Ma, Wenjie and Zou, Shanzi and Chen, Biao and Cheng, Hong and He, Xiaoxiao and Wang, Kemin
Journal: Talanta (2019): 152-158

Label-Free Telomerase Detection in Single Cell Using a Five-Base Telomerase Product-Triggered Exponential Rolling Circle Amplification Strategy.
Authors: Li, XiaoYu and Cui, YunXi and Du, YiChen and Tang, AnNa and Kong, DeMing
Journal: ACS sensors (2019): 1090-1096

Binding studies of a putative C. pseudotuberculosis target protein from Vitamin B12 Metabolism.
Authors: Peinado, Rafaela Dos S and Olivier, Danilo S and Eberle, Raphael J and de Moraes, Fabio R and Amaral, Marcos S and Arni, Raghuvir K and Coronado, Monika A
Journal: Scientific reports (2019): 6350

ELTA: Enzymatic Labeling of Terminal ADP-Ribose.
Authors: Ando, Yoshinari and Elkayam, Elad and McPherson, Robert Lyle and Dasovich, Morgan and Cheng, Shang-Jung and Voorneveld, Jim and Filippov, Dmitri V and Ong, Shao-En and Joshua-Tor, Leemor and Leung, Anthony K L
Journal: Molecular cell (2019): 845-856.e5

Gp41, a superfamily SF2 helicase from bacteriophage BFK20.
Authors: Halgasova, Nora and Matuskova, Radka and Kraus, Daniel and Tkacova, Adela and Balusikova, Lenka and Bukovska, Gabriela
Journal: Virus research (2018): 7-16

2-Allyl- and Propargylamino-dATPs for Site-Specific Enzymatic Introduction of a Single Modification in the Minor Groove of DNA.
Authors: Matyašovský, Ján and Pohl, Radek and Hocek, Michal
Journal: Chemistry (Weinheim an der Bergstrasse, Germany) (2018): 14938-14941

DNA Primer Extension with Cyclopropenylated 7-Deaza-2'-deoxyadenosine and Efficient Bioorthogonal Labeling in Vitro and in Living Cells.
Authors: Ploschik, Damian and Rönicke, Franziska and Beike, Hanna and Strasser, Ralf and Wagenknecht, Hans-Achim
Journal: Chembiochem : a European journal of chemical biology (2018): 1949-1953