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Cyanine 5-dATP [Cy5-dATP]

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Physical properties
Molecular weight1182.05
Spectral properties
Correction Factor (260 nm)0.02
Correction Factor (280 nm)0.03
Correction Factor (482 nm)0.009
Correction Factor (565 nm)0.09
Extinction coefficient (cm -1 M -1)2500001
Excitation (nm)651
Emission (nm)670
Quantum yield0.271, 0.42
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure


Molecular weight
Correction Factor (260 nm)
Correction Factor (280 nm)
Correction Factor (482 nm)
Correction Factor (565 nm)
Extinction coefficient (cm -1 M -1)
Excitation (nm)
Emission (nm)
Quantum yield
0.271, 0.42
The dye-modified deoxyadenosine 5'-triphosphates are widely used for a variety of non-radioactive DNA labeling reactions including nick translation, random prime labeling, cDNA labeling and 3’-end labeling. Cy5-dATP can be used to produce Cy5-containing DNA by conventional enzymatic incorporation methods such as reverse transcription, nick translation, random primed labeling, or PCR. This enzymatic fluorescence labeling method is widely used for both FISH probes and microarray-based experiments. This Cy5-dATP conjugate can be used as a red fluorescence color with SpectrumFRed™ filter set (SpectrumFRed™ is the trademark of Vysis). It is functionally equivalent to Cyanine 5-dATP (Perkin Elmer’s NEL593001EA) with significantly improved stability.


Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of Cyanine 5-dATP [Cy5-dATP] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM84.599 µL422.994 µL845.988 µL4.23 mL8.46 mL
5 mM16.92 µL84.599 µL169.198 µL845.988 µL1.692 mL
10 mM8.46 µL42.299 µL84.599 µL422.994 µL845.988 µL

Molarity calculator

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Spectral properties

Correction Factor (260 nm)0.02
Correction Factor (280 nm)0.03
Correction Factor (482 nm)0.009
Correction Factor (565 nm)0.09
Extinction coefficient (cm -1 M -1)2500001
Excitation (nm)651
Emission (nm)670
Quantum yield0.271, 0.42

Product Family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (260 nm)Correction Factor (280 nm)Correction Factor (482 nm)Correction Factor (565 nm)
Cyanine 5 monoacid [equivalent to Cy5® acid]65167025000010.271, 0.420.020.030.0090.09
Cyanine 5 maleimide [equivalent to Cy5® maleimide]65167025000010.271, 0.420.020.030.0090.09
Cyanine 5 azide [equivalent to Cy5® azide]65167025000010.271, 0.420.020.030.0090.09
Cyanine 5 alkyne [equivalent to Cy5® alkyne]65167025000010.271, 0.420.020.030.0090.09
Cyanine 5 amine [equivalent to Cy5® amine]65167025000010.271, 0.420.020.030.0090.09
Cyanine 5 hydrazide [equivalent to Cy5® hydrazide]65167025000010.271, 0.420.020.030.0090.09
Cyanine 5 bisacid [equivalent to Cy5® bisacid]65167025000010.271, 0.420.020.030.0090.09
Cyanine-5- dUTP [Cy5-dUTP] *1 mM in TE Buffer (pH 7.5)*65167025000010.271, 0.420.020.030.0090.09



View all 1 citations: Citation Explorer
Probe-based qPCR: Probe Compatibility and Multiplexing with Luna{\textregistered} Universal Probe qPCR Master Mix
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DNA Primer Extension with Cyclopropenylated 7-Deaza-2'-deoxyadenosine and Efficient Bioorthogonal Labeling in Vitro and in Living Cells.
Authors: Ploschik, Damian and Rönicke, Franziska and Beike, Hanna and Strasser, Ralf and Wagenknecht, Hans-Achim
Journal: Chembiochem : a European journal of chemical biology (2018): 1949-1953
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Journal: Chemistry (Weinheim an der Bergstrasse, Germany) (2018): 14938-14941