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Cyanine 5-dATP [Cy5-dATP]

The dye-modified deoxyadenosine 5'-triphosphates are widely used for a variety of non-radioactive DNA labeling reactions including nick translation, random prime labeling, cDNA labeling and 3’-end labeling. Cy5-dATP can be used to produce Cy5-containing DNA by conventional enzymatic incorporation methods such as reverse transcription, nick translation, random primed labeling, or PCR. This enzymatic fluorescence labeling method is widely used for both FISH probes and microarray-based experiments. This Cy5-dATP conjugate can be used as a red fluorescence color with SpectrumFRed™ filter set (SpectrumFRed™ is the trademark of Vysis). It is functionally equivalent to Cyanine 5-dATP (Perkin Elmer’s NEL593001EA) with significantly improved stability.

Spectrum

Product family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (260 nm)Correction Factor (280 nm)Correction Factor (482 nm)Correction Factor (565 nm)
Cyanine 5 monoacid [equivalent to Cy5® acid]65167025000010.271, 0.420.020.030.0090.09
Cyanine 5 maleimide [equivalent to Cy5® maleimide]65167025000010.271, 0.420.020.030.0090.09
Cyanine 5 azide [equivalent to Cy5® azide]65167025000010.271, 0.420.020.030.0090.09
Cyanine 5 alkyne [equivalent to Cy5® alkyne]65167025000010.271, 0.420.020.030.0090.09
Cyanine 5 amine [equivalent to Cy5® amine]65167025000010.271, 0.420.020.030.0090.09
Cyanine 5 hydrazide [equivalent to Cy5® hydrazide]65167025000010.271, 0.420.020.030.0090.09
Cyanine 5 bisacid [equivalent to Cy5® bisacid]65167025000010.271, 0.420.020.030.0090.09
Cyanine-5- dUTP [Cy5-dUTP] *1 mM in TE Buffer (pH 7.5)*65167025000010.271, 0.420.020.030.0090.09

Citations

View all 1 citations: Citation Explorer
Probe-based qPCR: Probe Compatibility and Multiplexing with Luna{\textregistered} Universal Probe qPCR Master Mix
Authors: Tanner, Nathan A and Gray, Andrew N and Nichols, Nicole M
Journal: Probe : 17

References

View all 50 references: Citation Explorer
New bioluminescence pyrophosphate assay for high-sensitivity detection of food-borne pathogens.
Authors: Fei, Zhongjie and Zhou, Dongrui and Li, Na and Xiao, Pengfeng
Journal: Luminescence : the journal of biological and chemical luminescence (2020): 355-364
2-Formyl-dATP as Substrate for Polymerase Synthesis of Reactive DNA Bearing an Aldehyde Group in the Minor Groove.
Authors: Krömer, Matouš and Brunderová, Mária and Ivancová, Ivana and Poštová Slavětínská, Lenka and Hocek, Michal
Journal: ChemPlusChem (2020): 1164-1170
Fluorescent dATP for DNA Synthesis In Vivo.
Authors: Schreier, Verena N and Loehr, Morten O and Deng, Ting and Lattmann, Evelyn and Hajnal, Alex and Neuhauss, Stephan C F and Luedtke, Nathan W
Journal: ACS chemical biology (2020): 2996-3003
Binding studies of a putative C. pseudotuberculosis target protein from Vitamin B12 Metabolism.
Authors: Peinado, Rafaela Dos S and Olivier, Danilo S and Eberle, Raphael J and de Moraes, Fabio R and Amaral, Marcos S and Arni, Raghuvir K and Coronado, Monika A
Journal: Scientific reports (2019): 6350
ELTA: Enzymatic Labeling of Terminal ADP-Ribose.
Authors: Ando, Yoshinari and Elkayam, Elad and McPherson, Robert Lyle and Dasovich, Morgan and Cheng, Shang-Jung and Voorneveld, Jim and Filippov, Dmitri V and Ong, Shao-En and Joshua-Tor, Leemor and Leung, Anthony K L
Journal: Molecular cell (2019): 845-856.e5
Page updated on November 3, 2024

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Catalog Number17038
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Physical properties

Molecular weight

1182.05

Solvent

Water

Spectral properties

Correction Factor (260 nm)

0.02

Correction Factor (280 nm)

0.03

Correction Factor (482 nm)

0.009

Correction Factor (565 nm)

0.09

Extinction coefficient (cm -1 M -1)

2500001

Excitation (nm)

651

Emission (nm)

670

Quantum yield

0.271, 0.42

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12171501