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Hydroxyl Radical Detection

The detection of intracellular hydroxyl radical is of central importance to the understanding of proper cellular redox regulation and the impact of its dysregulation on various pathologies. The hydroxyl radical (•OH) is one of the reactive oxygen species (ROS) that are highly reactive with other molecules to achieve stability. In general, hydroxyl radical is considered to be a harmful by-product of oxidative metabolism, which can cause molecular damage in living systems. It shows an average lifetime of 10-9 ns and can react with nearly every biomolecule, such as nuclear DNA, mitochondrial DNA, proteins and membrane lipids.

Cell Meter™ Mitochondrial Hydroxyl Radical Detection Kit (Cat# 16055) is optimized for detecting hydroxyl radical in mitochondria. MitoROS™ OH580 used in the kit is a live-cell permeant probe that can rapidly and selectively target hydroxyl radical in live cells. It generates red fluorescence when it reacts with •OH, and can be easily read at Ex/Em= 540/590 nm. Cell Meter™ Mitochondrial Hydroxyl Radical Detection Kit provides a sensitive fluorimetric probe to detect •OH in live cells with one hour incubation. This kit can be used for fluorescence microplate readers and fluorescence microscopy applications.


Fluorescence images of hydroxyl radical measurements in HeLa cells using Cell Meter™ Mitochondrial Hydroxyl Radical Detection Kit (Cat# 16055). Control (Top): HeLa cells were kept in 1X HBSS buffer without treatment. Cell nuclei were stained with Hoechst 33342 (Blue, Cat# 17530). Fenton Reaction (Bottom): Cells were treated with 10 µM CuCI2 and 100 µM H2O2 in 1X HBSS buffer at 37 °C for 1 hour.

 

Table 1. Hydroxyl Radical Assay Kit

Cat No.
Product Name
Ex (nm)
Em (nm)
Unit Size
Price
16055Cell Meter™ Mitochondrial Hydroxyl Radical Detection Kit *Red Fluorescence*576598200 Tests$327