AAT Bioquest

AssayWise Letters 2015, Vol. 4(2)

Catalase Detection
Catalase is an enzyme that protects the cell from oxidative damage by catalyzing the decomposition of hydrogen peroxide into water and oxygen. By preventing the excessive buildup of Hâ‚‚Oâ‚‚, catalase allows important cellular processes which produce H2O2 as a by-product to take place safely. AAT

Peroxidase Detection
Peroxidase is a small molecule (MW ~40 KD) that can usually be conjugated to an antibody. HRP conjugates are extensively used as secondary detection reagents in ELISAs, immuno-histochemical techniques as well as Northern, Southern and Western blot analyses. Our Amplite™ Fluorimetric Peroxidase

Hydroxyl Radical Detection
The detection of intracellular hydroxyl radical is of central importance to the understanding of proper cellular redox regulation and the impact of its dysregulation on various pathologies. In general, the hydroxyl radical is considered to be a harmful by-product of oxidative metabolism which

Superoxide Detection
Hydroethidine (Cat# 15200), a redox-sensitive probe, has been widely used to detect intracellular superoxide anion. It is a common assumption that the reaction between superoxide and hydroethidine results in the formation of a two-electron oxidized product, ethidium, which binds to DNA and leads

SOD Detection
Superoxide dismutases (SODs) are a class of enzymes that catalyze the dismutation of superoxide into oxygen and hydrogen peroxide. SODs are an important antioxidant defense in nearly all cells exposed to superoxide radicals. Amplite™ Colorimetric Superoxide Dismutase (SOD) Assay Kit (Cat# 11305)

Xanthine Assay
Xanthine oxidase (XO) is an enzyme normally found in the liver and jejunum. During severe liver damage, xanthine oxidase is released into blood. Xanthine oxidase also catalyzes the oxidation of hypoxanthine to xanthine, a purine base found in most human body tissues and fluids. A number of stimulants

Hypochlorite Assay
Hypochlorite anion (ClO-) and its protonated form, hypochlorous acid (HClO) are critical reactive oxygen species (ROS) in biological systems. Uncontrolled production of hypochlorite (hypochlorous acid) can lead to tissue damage and diseases including arthritis, renal failure and cancers.

Total ROS Detection
Detecting ROS activity is readily accessible through our ROS Brite™ & MitoROS™ reagents and Cell Meter™ Fluorimetric Intracellular Total ROS Activity Assay Kits.

Thiol Detection
The monitoring of reduced and oxidized glutathione (GSH) in biological samples is essential for evaluating the redox and detoxification status of cells and tissues in relation to the protective role of glutathione against oxidative and free-radical-mediated cell injury. Quantifying GSH and GSSG

Nitric Oxide Detection
Nitric oxide (NO) free radical is an important biological regulator and is therefore a fundamental component in the fields of neuroscience, physiology, and immunology. We have optimized our 3 multicolor DAX-J2™ reagents for NO detection in flow cytometric and fluorescence microscopy applications