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AssayWise Letters 2015, Vol. 4(2)
In this issue
Hydrogen Peroxide Detection
Catalase Detection
Peroxidase Detection
Hydroxyl Radical Detection
Superoxide Detection
SOD Detection
Xanthine Assay
Hypochlorite Assay
Total ROS Detection
Thiol Detection
Nitric Oxide Detection
AssayWise Letters 2015, Vol. 4(2)
Featured Article
Hydrogen Peroxide Detection
Hydrogen peroxide (Hâ‚‚Oâ‚‚) is a reactive oxygen metabolic by-product that serves as a key regulator for a number of oxidative stress-related states. Measurement of this reactive species will help to determine how oxidative stress modulates varied intracellular pathways. Our Amplite™ Fluorimetric Hydrogen Peroxide Assay Kits (Cat# 11502 & 11501) provide researchers with tools to quantify hydrogen peroxide in solutions and cell extracts. These kits may also be used to detect a variety of oxidase activities through enzyme-coupled reactions.…
Hydrogen peroxide (Hâ‚‚Oâ‚‚) is a reactive oxygen metabolic by-product that serves as a key regulator for a number of oxidative stress-related states. Measurement of this reactive species will help to determine how oxidative stress modulates varied intracellular pathways. Our Amplite™ Fluorimetric Hydrogen Peroxide Assay Kits (Cat# 11502 & 11501) provide researchers with tools to quantify hydrogen peroxide in solutions and cell extracts. These kits may also be used to detect a variety of oxidase activities through enzyme-coupled reactions.…
Catalase Detection
Catalase is an enzyme that protects the cell from oxidative damage by catalyzing the decomposition of hydrogen peroxide into water and oxygen. By preventing the excessive buildup of Hâ‚‚Oâ‚‚, catalase allows important cellular processes which produce H2O2 as a by-product to take place safely. AAT…
Catalase is an enzyme that protects the cell from oxidative damage by catalyzing the decomposition of hydrogen peroxide into water and oxygen. By preventing the excessive buildup of Hâ‚‚Oâ‚‚, catalase allows important cellular processes which produce H2O2 as a by-product to take place safely. AAT…
Peroxidase Detection
Peroxidase is a small molecule (MW ~40 KD) that can usually be conjugated to an antibody. HRP conjugates are extensively used as secondary detection reagents in ELISAs, immuno-histochemical techniques as well as Northern, Southern and Western blot analyses. Our Amplite™ Fluorimetric Peroxidase…
Peroxidase is a small molecule (MW ~40 KD) that can usually be conjugated to an antibody. HRP conjugates are extensively used as secondary detection reagents in ELISAs, immuno-histochemical techniques as well as Northern, Southern and Western blot analyses. Our Amplite™ Fluorimetric Peroxidase…
Hydroxyl Radical Detection
The detection of intracellular hydroxyl radical is of central importance to the understanding of proper cellular redox regulation and the impact of its dysregulation on various pathologies. In general, the hydroxyl radical is considered to be a harmful by-product of oxidative metabolism which…
The detection of intracellular hydroxyl radical is of central importance to the understanding of proper cellular redox regulation and the impact of its dysregulation on various pathologies. In general, the hydroxyl radical is considered to be a harmful by-product of oxidative metabolism which…
Superoxide Detection
Hydroethidine (Cat# 15200), a redox-sensitive probe, has been widely used to detect intracellular superoxide anion. It is a common assumption that the reaction between superoxide and hydroethidine results in the formation of a two-electron oxidized product, ethidium, which binds to DNA and leads…
Hydroethidine (Cat# 15200), a redox-sensitive probe, has been widely used to detect intracellular superoxide anion. It is a common assumption that the reaction between superoxide and hydroethidine results in the formation of a two-electron oxidized product, ethidium, which binds to DNA and leads…
SOD Detection
Superoxide dismutases (SODs) are a class of enzymes that catalyze the dismutation of superoxide into oxygen and hydrogen peroxide. SODs are an important antioxidant defense in nearly all cells exposed to superoxide radicals. Amplite™ Colorimetric Superoxide Dismutase (SOD) Assay Kit (Cat# 11305)…
Superoxide dismutases (SODs) are a class of enzymes that catalyze the dismutation of superoxide into oxygen and hydrogen peroxide. SODs are an important antioxidant defense in nearly all cells exposed to superoxide radicals. Amplite™ Colorimetric Superoxide Dismutase (SOD) Assay Kit (Cat# 11305)…
Xanthine Assay
Xanthine oxidase (XO) is an enzyme normally found in the liver and jejunum. During severe liver damage, xanthine oxidase is released into blood. Xanthine oxidase also catalyzes the oxidation of hypoxanthine to xanthine, a purine base found in most human body tissues and fluids. A number of stimulants…
Xanthine oxidase (XO) is an enzyme normally found in the liver and jejunum. During severe liver damage, xanthine oxidase is released into blood. Xanthine oxidase also catalyzes the oxidation of hypoxanthine to xanthine, a purine base found in most human body tissues and fluids. A number of stimulants…
Hypochlorite Assay
Hypochlorite anion (ClO-) and its protonated form, hypochlorous acid (HClO) are critical reactive oxygen species (ROS) in biological systems. Uncontrolled production of hypochlorite (hypochlorous acid) can lead to tissue damage and diseases including arthritis, renal failure and cancers.…
Hypochlorite anion (ClO-) and its protonated form, hypochlorous acid (HClO) are critical reactive oxygen species (ROS) in biological systems. Uncontrolled production of hypochlorite (hypochlorous acid) can lead to tissue damage and diseases including arthritis, renal failure and cancers.…
Total ROS Detection
Detecting ROS activity is readily accessible through our ROS Brite™ & MitoROS™ reagents and Cell Meter™ Fluorimetric Intracellular Total ROS Activity Assay Kits.…
Detecting ROS activity is readily accessible through our ROS Brite™ & MitoROS™ reagents and Cell Meter™ Fluorimetric Intracellular Total ROS Activity Assay Kits.…
Thiol Detection
The monitoring of reduced and oxidized glutathione (GSH) in biological samples is essential for evaluating the redox and detoxification status of cells and tissues in relation to the protective role of glutathione against oxidative and free-radical-mediated cell injury. Quantifying GSH and GSSG…
The monitoring of reduced and oxidized glutathione (GSH) in biological samples is essential for evaluating the redox and detoxification status of cells and tissues in relation to the protective role of glutathione against oxidative and free-radical-mediated cell injury. Quantifying GSH and GSSG…
Nitric Oxide Detection
Nitric oxide (NO) free radical is an important biological regulator and is therefore a fundamental component in the fields of neuroscience, physiology, and immunology. We have optimized our 3 multicolor DAX-J2™ reagents for NO detection in flow cytometric and fluorescence microscopy applications…
Nitric oxide (NO) free radical is an important biological regulator and is therefore a fundamental component in the fields of neuroscience, physiology, and immunology. We have optimized our 3 multicolor DAX-J2™ reagents for NO detection in flow cytometric and fluorescence microscopy applications…