AAT Bioquest

Catalase Detection

Catalase does responses were measured with Amplite<sup>®</sup> Fluorimetric Catalase Assasy Kit
Catalase does responses were measured with Amplite® Fluorimetric Catalase Assasy Kit (Cat# 11306) in a 96-well solid black plate using a Gemini fluorescence microplate reader. As low as 30 mU/mL catalase was detected with 30 minutes incubation (n=3).
Catalase is a common antioxidant heme-containing redox enzyme found in nearly all living organisms that are exposed to oxygen. The enzyme is concentrated in the peroxisome subcellular organelles. Hydrogen peroxide is a reactive oxygen species (ROS) that is a toxic product of normal aerobic metabolism and pathogenic ROS production involving oxidase and superoxide dismutase reactions. By preventing the excessive buildup of H2O2, catalase allows important cellular processes which produce H2O2 as a by-product to take place safely

Amplite® Fluorimetric Catalase Assay Kit (Cat# 11306) provides a quick and sensitive method for the measurement of catalase activity. Catalase reacts with H2O2 to produce water and oxygen (O2). Amplite® Red used in the assay kit reacts with H2O2 to generate a red fluorescent product. Therefore the reduction in fluorescence intensity is proportional to catalase activity. Amplite® Red enables a dual recordable mode. The fluorescent signal can be easily read by either a fluorescence microplate reader at Ex/Em = 540/590 nm or an absorbance microplate reader at ~576 nm. With Amplite® Fluorimetric Catalase Assay Kit, as low as 30 mU/mL catalase was detected in a 100 µL reaction volume.

Table 1. Catalase Detection Assay Kit

Cat No.
Product Name
Ex (nm)
Em (nm)
Unit Size
11306Amplite® Fluorimetric Catalase Assay Kit *Red Fluorescence*571585200 Tests